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HF®, Nicking, Master Mix, Time-Saver & Other

The Leader in the Discovery and Production of Restriction Enzymes

After nearly 40 years of offering restriction enzymes to the research community, NEB has earned the reputation of being a leader in enzyme technologies. Working continuously to be worthy of that distinction, NEB strives to develop enzymes of the highest purity and unparalleled performance.

NEB scientists continue to improve its portfolio of restriction enzymes, as well as explore their utility in new technologies. As a result, NEB scientists continue to publish scientific papers and to be awarded grants in this area. With the industry’s largest research and development group dedicated to restriction enzymes, we are proud to have been there first: the first to commercialize a recombinant enzyme, the first to introduce a nicking enzyme, and the first to supply a true restriction enzyme master mix. In addition, NEB has an ongoing history of innovation by engineering restriction enzymes with altered specificities and improved performance. Through continued research in these areas, we are committed to driving the innovations that allow us to offer maximum convenience and performance.


HF®, Nicking, Master Mix, Time-Saver & Other

High-Fidelity (HF®) Restriction Endonucleases

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Order AgeI-HF®

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Order ApoI-HF®

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Order BamHI-HF®

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Order BbsI-HF®

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Order BclI-HF

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Order BmtI-HF®

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Order BsaI-HF®

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Order BsiWI-HF®

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Order BsrGI-HF®

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Order BstEII-HF®

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Order BstZ17I-HF®

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Order DraIII-HF®

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Order EagI-HF®

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Order EcoRI-HF®

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Order EcoRV-HF®

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Order HindIII-HF®

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Order KpnI-HF®

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Order MfeI-HF®

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Order MluI-HF®

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Order NcoI-HF®

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Order NheI-HF®

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Order NotI-HF®

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Order NruI-HF®

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Order NsiI-HF®

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Order PstI-HF®

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Order PvuI-HF®

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Order PvuII-HF®

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Order SacI-HF®

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Order SalI-HF®

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Order SbfI-HF®

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Order ScaI-HF®

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Order SpeI-HF®

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Order SphI-HF®

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Order SspI-HF®

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Order StyI-HF®

Homing Endonucleases

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Order I-CeuI

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Order I-SceI

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Order PI-PspI

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Order PI-SceI

Nicking Endonucleases

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Order Nb.BbvCI

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Order Nb.BsmI

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Order Nb.BsrDI

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Order Nb.BssSI

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Order Nb.BtsI

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Order Nt.AlwI

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Order Nt.BbvCI

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Order Nt.BsmAI

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Order Nt.BspQI

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Order Nt.BstNBI

Restriction Enzymes for Epigenetic Analysis (EpiMark® Validated)

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Order AbaSI

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Order DpnI

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Order DpnII

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Order FspEI

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Order HpaII

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Order LpnPI

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Order McrBC

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Order MspI

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Order MspJI

Time-Saver™ Qualified Restriction Enzymes

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Order AatII

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Order Acc65I

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Order AccI

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Order AciI

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Order AclI

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Order AcuI

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Order AflII

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Order AgeI-HF®

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Order AhdI

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Order AluI

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Order AlwNI

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Order ApaI

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Order ApaLI

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Order ApeKI

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Order ApoI-HF®

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Order AscI

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Order AseI

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Order AvaI

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Order AvaII

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Order AvrII

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Order BaeGI

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Order BaeI

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Order BamHI

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Order BamHI-HF®

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Order BbsI

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Order BbsI-HF®

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Order BbvI

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Order BciVI

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Order BclI

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Order BclI-HF

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Order BcoDI

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Order BfuAI

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Order BglI

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Order BglII

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Order BlpI

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Order BmgBI

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Order BmtI-HF®

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Order BpmI

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Order BpuEI

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Order BsaAI

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Order BsaHI

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Order BsaI-HF®

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Order BsaWI

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Order BsaXI

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Order BseRI

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Order BsgI

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Order BsiEI

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Order BsiWI

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Order BsiWI-HF®

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Order BslI

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Order BsmAI

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Order BsmBI

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Order BsmI

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Order BsoBI

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Order Bsp1286I

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Order BspCNI

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Order BspEI

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Order BspHI

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Order BspQI

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Order BsrBI

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Order BsrDI

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Order BsrGI

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Order BsrI

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Order BssHII

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Order BstBI

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Order BstEII

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Order BstEII-HF®

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Order BstNI

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Order BstUI

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Order BstXI

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Order BstYI

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Order BstZ17I-HF®

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Order Bsu36I

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Order BtgI

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Order BtsCI

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Order Cac8I

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Order ClaI

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Order CspCI

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Order CviAII

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Order CviQI

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Order DdeI

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Order DpnI

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Order DpnII

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Order DraI

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Order DraIII-HF®

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Order DrdI

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Order EagI

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Order EagI-HF®

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Order EarI

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Order Eco53kI

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Order EcoNI

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Order EcoO109I

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Order EcoP15I

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Order EcoRI

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Order EcoRI-HF®

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Order EcoRV

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Order EcoRV-HF®

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Order Fnu4HI

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Order FseI

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Order FspI

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Order HaeIII

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Order HhaI

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Order HincII

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Order HindIII-HF®

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Order HinfI

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Order HinP1I

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Order HpaII

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Order HphI

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Order Hpy166II

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Order HpyAV

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Order HpyCH4IV

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Order HpyCH4V

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Order KpnI

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Order KpnI-HF®

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Order MboI

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Order MfeI-HF®

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Order MluCI

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Order MluI

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Order MlyI

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Order MmeI

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Order MnlI

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Order MseI

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Order MslI

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Order MspA1I

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Order MspI

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Order MwoI

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Order NciI

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Order NcoI

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Order NcoI-HF®

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Order NdeI

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Order NgoMIV

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Order NheI

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Order NheI-HF®

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Order NlaIII

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Order NotI

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Order NotI-HF®

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Order NruI

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Order NsiI

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Order NspI

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Order PacI

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Order PaeR7I

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Order PflFI

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Order PflMI

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Order PmeI

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Order PpuMI

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Order PshAI

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Order PstI

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Order PstI-HF®

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Order PvuI

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Order PvuI-HF®

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Order PvuII

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Order PvuII-HF®

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Order RsaI

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Order SacI

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Order SacI-HF®

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Order SacII

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Order SalI

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Order SalI-HF®

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Order SapI

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Order SbfI

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Order SbfI-HF®

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Order ScaI-HF®

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Order SfiI

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Order SfoI

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Order SmaI

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Order SpeI

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Order SpeI-HF®

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Order SphI-HF®

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Order SspI-HF®

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Order StuI

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Order StyD4I

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Order StyI

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Order StyI-HF®

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Order SwaI

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Order TaqαI

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Order TfiI

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Order TseI

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Order TspMI

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Order TspRI

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Order Tth111I

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Order XbaI

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Order XhoI

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Order XmaI

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Order XmnI

High-Fidelity (HF®) Restriction Endonucleases

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Order AgeI-HF®

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Order ApoI-HF®

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Order BamHI-HF®

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Order BbsI-HF®

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Order BclI-HF

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Order BmtI-HF®

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Order BsaI-HF®

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Order BsiWI-HF®

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Order BsrGI-HF®

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Order BstEII-HF®

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Order BstZ17I-HF®

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Order DraIII-HF®

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Order EagI-HF®

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Order EcoRI-HF®

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Order EcoRV-HF®

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Order HindIII-HF®

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Order KpnI-HF®

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Order MfeI-HF®

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Order MluI-HF®

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Order NcoI-HF®

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Order NheI-HF®

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Order NotI-HF®

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Order NruI-HF®

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Order NsiI-HF®

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Order PstI-HF®

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Order PvuI-HF®

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Order PvuII-HF®

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Order SacI-HF®

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Order SalI-HF®

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Order SbfI-HF®

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Order ScaI-HF®

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Order SpeI-HF®

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Order SphI-HF®

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Order SspI-HF®

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Order StyI-HF®

Homing Endonucleases

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Order I-CeuI

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Order I-SceI

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Order PI-PspI

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Order PI-SceI

Nicking Endonucleases

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Order Nb.BbvCI

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Order Nb.BsmI

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Order Nb.BsrDI

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Order Nb.BssSI

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Order Nb.BtsI

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Order Nt.AlwI

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Order Nt.BbvCI

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Order Nt.BsmAI

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Order Nt.BspQI

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Order Nt.BstNBI

Restriction Enzymes for Epigenetic Analysis (EpiMark® Validated)

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Order AbaSI

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Order DpnI

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Order DpnII

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Order FspEI

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Order HpaII

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Order LpnPI

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Order McrBC

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Order MspI

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Order MspJI

Time-Saver™ Qualified Restriction Enzymes

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Order AatII

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Order Acc65I

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Order AccI

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Order AciI

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Order AclI

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Order AcuI

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Order AflII

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Order AgeI-HF®

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Order AhdI

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Order AluI

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Order AlwNI

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Order ApaI

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Order ApaLI

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Order ApeKI

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Order ApoI-HF®

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Order AscI

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Order AseI

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Order AvaI

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Order AvaII

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Order AvrII

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Order BaeGI

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Order BaeI

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Order BamHI

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Order BamHI-HF®

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Order BbsI

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Order BbsI-HF®

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Order BbvI

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Order BciVI

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Order BclI

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Order BclI-HF

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Order BcoDI

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Order BfuAI

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Order BglI

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Order BglII

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Order BlpI

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Order BmgBI

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Order BmtI-HF®

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Order BpmI

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Order BpuEI

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Order BsaAI

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Order BsaHI

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Order BsaI-HF®

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Order BsaWI

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Order BsaXI

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Order BseRI

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Order BsgI

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Order BsiEI

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Order BsiWI

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Order BsiWI-HF®

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Order BslI

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Order BsmAI

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Order BsmBI

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Order BsmI

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Order BsoBI

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Order Bsp1286I

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Order BspCNI

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Order BspEI

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Order BspHI

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Order BspQI

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Order BsrBI

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Order BsrDI

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Order BsrGI

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Order BsrI

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Order BssHII

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Order BstBI

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Order BstEII

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Order BstEII-HF®

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Order BstNI

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Order BstUI

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Order BstXI

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Order BstYI

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Order BstZ17I-HF®

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Order Bsu36I

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Order BtgI

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Order BtsCI

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Order Cac8I

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Order ClaI

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Order CspCI

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Order CviAII

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Order CviQI

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Order DdeI

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Order DpnI

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Order DpnII

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Order DraI

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Order DraIII-HF®

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Order DrdI

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Order EagI

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Order EagI-HF®

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Order EarI

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Order Eco53kI

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Order EcoNI

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Order EcoO109I

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Order EcoP15I

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Order EcoRI

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Order EcoRI-HF®

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Order EcoRV

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Order EcoRV-HF®

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Order Fnu4HI

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Order FseI

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Order FspI

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Order HaeIII

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Order HhaI

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Order HincII

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Order HindIII-HF®

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Order HinfI

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Order HinP1I

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Order HpaII

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Order HphI

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Order Hpy166II

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Order HpyAV

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Order HpyCH4IV

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Order HpyCH4V

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Order KpnI

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Order KpnI-HF®

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Order MboI

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Order MfeI-HF®

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Order MluCI

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Order MluI

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Order MlyI

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Order MmeI

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Order MnlI

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Order MseI

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Order MslI

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Order MspA1I

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Order MspI

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Order MwoI

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Order NciI

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Order NcoI

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Order NcoI-HF®

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Order NdeI

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Order NgoMIV

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Order NheI

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Order NheI-HF®

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Order NlaIII

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Order NotI

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Order NotI-HF®

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Order NruI

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Order NsiI

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Order NspI

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Order PacI

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Order PaeR7I

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Order PflFI

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Order PflMI

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Order PmeI

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Order PpuMI

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Order PshAI

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Order PstI

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Order PstI-HF®

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Order PvuI

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Order PvuI-HF®

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Order PvuII

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Order PvuII-HF®

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Order RsaI

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Order SacI

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Order SacI-HF®

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Order SacII

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Order SalI

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Order SalI-HF®

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Order SapI

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Order SbfI

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Order SbfI-HF®

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Order ScaI-HF®

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Order SfiI

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Order SfoI

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Order SmaI

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Order SpeI

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Order SpeI-HF®

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Order SphI-HF®

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Order SspI-HF®

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Order StuI

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Order StyD4I

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Order StyI

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Order StyI-HF®

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Order SwaI

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Order TaqαI

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Order TfiI

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Order TseI

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Order TspMI

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Order TspRI

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Order Tth111I

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Order XbaI

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Order XhoI

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Order XmaI

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Order XmnI