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HF®, Nicking, Master Mix, Time-Saver & Other Products

The Leader in the Discovery and Production of Restriction Enzymes

After nearly 40 years of offering restriction enzymes to the research community, NEB has earned the reputation of being a leader in enzyme technologies. Working continuously to be worthy of that distinction, NEB strives to develop enzymes of the highest purity and unparalleled performance.

NEB scientists continue to improve its portfolio of restriction enzymes, as well as explore their utility in new technologies. As a result, NEB scientists continue to publish scientific papers and to be awarded grants in this area. With the industry’s largest research and development group dedicated to restriction enzymes, we are proud to have been there first: the first to commercialize a recombinant enzyme, the first to introduce a nicking enzyme, and the first to supply a true restriction enzyme master mix. In addition, NEB has an ongoing history of innovation by engineering restriction enzymes with altered specificities and improved performance. Through continued research in these areas, we are committed to driving the innovations that allow us to offer maximum convenience and performance.



Choose Product:

HF®, Nicking, Master Mix, Time-Saver & Other

High-Fidelity (HF®) Restriction Endonucleases

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AgeI-HF®

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ApoI-HF®

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BamHI-HF®

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BbsI-HF®

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BclI-HF

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BmtI-HF®

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BsaI-HF®v2

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BsiWI-HF®

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BsrGI-HF®

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BstEII-HF®

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BstZ17I-HF®

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DraIII-HF®

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EagI-HF®

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EcoRI-HF®

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EcoRV-HF®

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HindIII-HF®

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KpnI-HF®

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MfeI-HF®

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MluI-HF®

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NcoI-HF®

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NheI-HF®

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NotI-HF®

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NruI-HF®

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NsiI-HF®

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PstI-HF®

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PvuI-HF®

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PvuII-HF®

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SacI-HF®

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SalI-HF®

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SbfI-HF®

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ScaI-HF®

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SpeI-HF®

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SphI-HF®

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SspI-HF®

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StyI-HF®

Homing Endonucleases

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I-CeuI

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I-SceI

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PI-PspI

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PI-SceI

Nicking Endonucleases

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Nb.BbvCI

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Nb.BsmI

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Nb.BsrDI

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Nb.BssSI

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Nb.BtsI

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Nt.AlwI

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Nt.BbvCI

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Nt.BsmAI

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Nt.BspQI

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Nt.BstNBI

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Nt.CviPII

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WarmStart® Nt.BstNBI

Restriction Enzymes for Epigenetic Analysis

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DpnI

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DpnII

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HpaII

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MspI

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MspJI

Time-Saver™ Qualified Restriction Enzymes

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AatII

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Acc65I

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AccI

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AciI

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AclI

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AcuI

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AflII

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AgeI-HF®

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AhdI

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AluI

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AlwNI

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ApaI

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ApaLI

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ApeKI

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ApoI-HF®

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AscI

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AseI

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AvaI

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AvaII

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AvrII

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BaeGI

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BaeI

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BamHI

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BamHI-HF®

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BbsI

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BbsI-HF®

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BbvI

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BciVI

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BclI

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BclI-HF

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BcoDI

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BfuAI

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BglI

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BglII

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BlpI

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BmgBI

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BmtI-HF®

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BpmI

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BpuEI

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BsaAI

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BsaHI

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BsaI-HF®v2

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BsaWI

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BsaXI

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BseRI

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BsgI

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BsiEI

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BsiWI

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BsiWI-HF®

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BslI

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BsmAI

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BsmBI-v2

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BsmI

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BsoBI

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Bsp1286I

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BspCNI

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BspEI

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BspHI

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BspQI

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BsrBI

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BsrDI

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BsrI

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BssHII

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BstBI

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BstEII-HF®

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BstNI

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BstUI

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BstXI

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BstYI

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BstZ17I-HF®

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Bsu36I

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BtgI

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BtsCI

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Cac8I

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ClaI

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CspCI

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CviQI

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DdeI

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DpnI

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DpnII

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DraI

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DraIII-HF®

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DrdI

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EagI-HF®

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EarI

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Eco53kI

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EcoNI

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EcoO109I

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EcoP15I

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EcoRI

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EcoRI-HF®

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EcoRV

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EcoRV-HF®

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Esp3I

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Fnu4HI

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FseI

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FspI

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HaeIII

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HhaI

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HincII

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HindIII-HF®

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HinfI

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HinP1I

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HpaII

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HphI

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Hpy166II

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HpyAV

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HpyCH4IV

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HpyCH4V

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KpnI-HF®

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MboI

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MfeI-HF®

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MluCI

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MlyI

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MmeI

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MnlI

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MseI

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MslI

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MspA1I

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MspI

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MwoI

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NciI

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NcoI

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NcoI-HF®

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NdeI

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NgoMIV

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NheI-HF®

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NlaIII

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NotI

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NotI-HF®

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NsiI

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NspI

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PacI

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PaeR7I

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PflFI

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PflMI

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PmeI

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PmlI

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PpuMI

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PshAI

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PstI

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PstI-HF®

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PvuI-HF®

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PvuII

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PvuII-HF®

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RsaI

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SacI-HF®

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SacII

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SalI

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SalI-HF®

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SapI

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SbfI-HF®

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ScaI-HF®

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SfiI

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SfoI

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SmaI

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SpeI-HF®

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SphI-HF®

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SspI-HF®

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StuI

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StyD4I

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StyI-HF®

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SwaI

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TaqI-v2

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TfiI

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TseI

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TspMI

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TspRI

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Tth111I

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XbaI

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XhoI

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XmaI

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XmnI

High-Fidelity (HF®) Restriction Endonucleases
Close

AgeI-HF®

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ApoI-HF®

Close

BamHI-HF®

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BbsI-HF®

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BclI-HF

Close

BmtI-HF®

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BsaI-HF®v2

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BsiWI-HF®

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BsrGI-HF®

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BstEII-HF®

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BstZ17I-HF®

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DraIII-HF®

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EagI-HF®

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EcoRI-HF®

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EcoRV-HF®

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HindIII-HF®

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KpnI-HF®

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MfeI-HF®

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MluI-HF®

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NcoI-HF®

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NheI-HF®

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NotI-HF®

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NruI-HF®

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NsiI-HF®

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PstI-HF®

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PvuI-HF®

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PvuII-HF®

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SacI-HF®

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SalI-HF®

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SbfI-HF®

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ScaI-HF®

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SpeI-HF®

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SphI-HF®

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SspI-HF®

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StyI-HF®

Homing Endonucleases
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I-CeuI

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I-SceI

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PI-PspI

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PI-SceI

Nicking Endonucleases
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Nb.BbvCI

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Nb.BsmI

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Nb.BsrDI

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Nb.BssSI

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Nb.BtsI

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Nt.AlwI

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Nt.BbvCI

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Nt.BsmAI

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Nt.BspQI

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Nt.BstNBI

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Nt.CviPII

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WarmStart® Nt.BstNBI

Restriction Enzymes for Epigenetic Analysis
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DpnI

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DpnII

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HpaII

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MspI

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MspJI

Time-Saver™ Qualified Restriction Enzymes
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AatII

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Acc65I

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AccI

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AciI

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AclI

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AcuI

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AflII

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AgeI-HF®

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AhdI

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AluI

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AlwNI

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ApaI

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ApaLI

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ApeKI

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ApoI-HF®

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AscI

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AseI

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AvaI

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AvaII

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AvrII

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BaeGI

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BaeI

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BamHI

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BamHI-HF®

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BbsI

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BbsI-HF®

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BbvI

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BciVI

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BclI

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BclI-HF

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BcoDI

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BfuAI

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BglI

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BglII

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BlpI

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BmgBI

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BmtI-HF®

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BpmI

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BpuEI

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BsaAI

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BsaHI

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BsaI-HF®v2

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BsaWI

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BsaXI

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BseRI

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BsgI

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BsiEI

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BsiWI

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BsiWI-HF®

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BslI

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BsmAI

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BsmBI-v2

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BsmI

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BsoBI

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Bsp1286I

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BspCNI

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BspEI

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BspHI

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BspQI

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BsrBI

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BsrDI

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BsrI

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BssHII

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BstBI

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BstEII-HF®

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BstNI

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BstUI

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BstXI

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BstYI

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BstZ17I-HF®

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Bsu36I

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BtgI

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BtsCI

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Cac8I

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ClaI

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CspCI

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CviQI

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DdeI

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DpnI

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DpnII

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DraI

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DraIII-HF®

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DrdI

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EagI-HF®

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EarI

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Eco53kI

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EcoNI

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EcoO109I

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EcoP15I

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EcoRI

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EcoRI-HF®

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EcoRV

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EcoRV-HF®

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Esp3I

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Fnu4HI

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FseI

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FspI

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HaeIII

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HhaI

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HincII

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HindIII-HF®

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HinfI

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HinP1I

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HpaII

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HphI

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Hpy166II

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HpyAV

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HpyCH4IV

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HpyCH4V

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KpnI-HF®

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MboI

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MfeI-HF®

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MluCI

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MlyI

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MmeI

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MnlI

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MseI

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MslI

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MspA1I

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MspI

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MwoI

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NciI

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NcoI

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NcoI-HF®

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NdeI

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NgoMIV

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NheI-HF®

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NlaIII

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NotI

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NotI-HF®

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NsiI

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NspI

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PacI

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PaeR7I

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PflFI

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PflMI

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PmeI

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PmlI

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PpuMI

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PshAI

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PstI

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PstI-HF®

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PvuI-HF®

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PvuII

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PvuII-HF®

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RsaI

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SacI-HF®

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SacII

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SalI

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SalI-HF®

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SapI

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SbfI-HF®

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ScaI-HF®

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SfiI

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SfoI

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SmaI

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SpeI-HF®

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SphI-HF®

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SspI-HF®

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StuI

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StyD4I

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StyI-HF®

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SwaI

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TaqI-v2

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TfiI

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TseI

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TspMI

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TspRI

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Tth111I

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XbaI

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XhoI

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XmaI

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XmnI