Restriction Endonucleases
  • My NEB
  • Print
  • PDF
  • HF®, Nicking, Master Mix, Time-Saver & Other

    The Leader in the Discovery and Production of Restriction Enzymes

    After nearly 40 years of offering restriction enzymes to the research community, NEB has earned the reputation of being a leader in enzyme technologies. Working continuously to be worthy of that distinction, NEB strives to develop enzymes of the highest purity and unparalleled performance.

    NEB scientists continue to improve its portfolio of restriction enzymes, as well as explore their utility in new technologies. As a result, NEB scientists continue to publish scientific papers and to be awarded grants in this area. With the industry’s largest research and development group dedicated to restriction enzymes, we are proud to have been there first: the first to commercialize a recombinant enzyme, the first to introduce a nicking enzyme, and the first to supply a true restriction enzyme master mix. In addition, NEB has an ongoing history of innovation by engineering restriction enzymes with altered specificities and improved performance. Through continued research in these areas, we are committed to driving the innovations that allow us to offer maximum convenience and performance.

    HF®, Nicking, Master Mix, Time-Saver & Other

    High-Fidelity (HF®) Restriction Endonucleases

    Close

    OrderAgeI-HF®

    Close

    OrderAgeI-HF® RE-Mix®

    Close

    OrderBamHI-HF®

    Close

    OrderBmtI-HF®

    Close

    OrderBsaI-HF®

    Close

    OrderBstEII-HF®

    Close

    OrderBstEII-HF® RE-Mix®

    Close

    OrderDraIII-HF®

    Close

    OrderEagI-HF®

    Close

    OrderEcoRI-HF®

    Close

    OrderEcoRI-HF® RE-Mix®

    Close

    OrderEcoRV-HF®

    Close

    OrderEcoRV-HF® RE-Mix®

    Close

    OrderHindIII-HF®

    Close

    OrderKpnI-HF®

    Close

    OrderKpnI-HF® RE-Mix®

    Close

    OrderMfeI-HF®

    Close

    OrderMfeI-HF® RE-Mix®

    Close

    OrderNcoI-HF®

    Close

    OrderNcoI-HF® RE-Mix®

    Close

    OrderNheI-HF®

    Close

    OrderNheI-HF® RE-Mix®

    Close

    OrderNotI-HF®

    Close

    OrderNotI-HF® RE-Mix®

    Close

    OrderPstI-HF®

    Close

    OrderPvuI-HF®

    Close

    OrderPvuII-HF®

    Close

    OrderSacI-HF®

    Close

    OrderSalI-HF®

    Close

    OrderSalI-HF® RE-Mix®

    Close

    OrderSbfI-HF®

    Close

    OrderScaI-HF®

    Close

    OrderScaI-HF® RE-Mix®

    Close

    OrderSpeI-HF®

    Close

    OrderSphI-HF®

    Close

    OrderSspI-HF®

    Close

    OrderStyI-HF®

    Homing Endonucleases

    Close

    OrderI-CeuI

    Close

    OrderI-SceI

    Close

    OrderPI-PspI

    Close

    OrderPI-SceI

    Nicking Endonucleases

    Close

    OrderNb.BbvCI

    Close

    OrderNb.BsmI

    Close

    OrderNb.BsrDI

    Close

    OrderNb.BtsI

    Close

    OrderNt.AlwI

    Close

    OrderNt.BbvCI

    Close

    OrderNt.BsmAI

    Close

    OrderNt.BspQI

    Close

    OrderNt.BstNBI

    Close

    OrderNt.CviPII

    RE-Mix® Restriction Enzyme Master Mixes

    Close

    OrderAgeI-HF® RE-Mix®

    Close

    OrderAscI RE-Mix®

    Close

    OrderBstEII-HF® RE-Mix®

    Close

    OrderEcoRI-HF® RE-Mix®

    Close

    OrderEcoRV-HF® RE-Mix®

    Close

    OrderKpnI-HF® RE-Mix®

    Close

    OrderMfeI-HF® RE-Mix®

    Close

    OrderNcoI-HF® RE-Mix®

    Close

    OrderNheI-HF® RE-Mix®

    Close

    OrderNotI-HF® RE-Mix®

    Close

    OrderPacI RE-Mix®

    Close

    OrderSalI-HF® RE-Mix®

    Close

    OrderScaI-HF® RE-Mix®

    Close

    OrderSpeI RE-Mix®

    Close

    OrderXbaI RE-Mix®

    Close

    OrderXhoI RE-Mix®

    Restriction Enzymes for Epigenetic Analysis (EpiMark® Validated)

    Close

    OrderDpnI

    Close

    OrderDpnII

    Close

    OrderFspEI

    Close

    OrderHpaII

    Close

    OrderLpnPI

    Close

    OrderMcrBC

    Close

    OrderMspI

    Close

    OrderMspJI

    Time-Saver™ Qualified Restriction Enzymes

    Close

    OrderAatII

    Close

    OrderAcc65I

    Close

    OrderAccI

    Close

    OrderAciI

    Close

    OrderAclI

    Close

    OrderAcuI

    Close

    OrderAflII

    Close

    OrderAgeI-HF®

    Close

    OrderAgeI-HF® RE-Mix®

    Close

    OrderAhdI

    Close

    OrderAluI

    Close

    OrderAlwNI

    Close

    OrderApaI

    Close

    OrderApaLI

    Close

    OrderApeKI

    Close

    OrderApoI

    Close

    OrderAscI

    Close

    OrderAscI RE-Mix®

    Close

    OrderAseI

    Close

    OrderAvaI

    Close

    OrderAvaII

    Close

    OrderAvrII

    Close

    OrderBaeGI

    Close

    OrderBaeI

    Close

    OrderBamHI

    Close

    OrderBamHI-HF®

    Close

    OrderBbsI

    Close

    OrderBbvI

    Close

    OrderBciVI

    Close

    OrderBclI

    Close

    OrderBcoDI

    Close

    OrderBfuAI

    Close

    OrderBfuCI

    Close

    OrderBglI

    Close

    OrderBglII

    Close

    OrderBlpI

    Close

    OrderBmgBI

    Close

    OrderBmtI-HF®

    Close

    OrderBpmI

    Close

    OrderBpuEI

    Close

    OrderBsaAI

    Close

    OrderBsaHI

    Close

    OrderBsaI-HF®

    Close

    OrderBsaWI

    Close

    OrderBsaXI

    Close

    OrderBseRI

    Close

    OrderBsgI

    Close

    OrderBsiEI

    Close

    OrderBsiWI

    Close

    OrderBslI

    Close

    OrderBsmAI

    Close

    OrderBsmBI

    Close

    OrderBsmI

    Close

    OrderBsoBI

    Close

    OrderBsp1286I

    Close

    OrderBspCNI

    Close

    OrderBspEI

    Close

    OrderBspHI

    Close

    OrderBspQI

    Close

    OrderBsrBI

    Close

    OrderBsrDI

    Close

    OrderBsrGI

    Close

    OrderBsrI

    Close

    OrderBssHII

    Close

    OrderBssKI

    Close

    OrderBstBI

    Close

    OrderBstEII

    Close

    OrderBstEII-HF®

    Close

    OrderBstEII-HF® RE-Mix®

    Close

    OrderBstNI

    Close

    OrderBstUI

    Close

    OrderBstXI

    Close

    OrderBstYI

    Close

    OrderBstZ17I

    Close

    OrderBsu36I

    Close

    OrderBtgI

    Close

    OrderBtsCI

    Close

    OrderCac8I

    Close

    OrderClaI

    Close

    OrderCspCI

    Close

    OrderCviAII

    Close

    OrderCviQI

    Close

    OrderDdeI

    Close

    OrderDpnI

    Close

    OrderDpnII

    Close

    OrderDraI

    Close

    OrderDraIII-HF®

    Close

    OrderDrdI

    Close

    OrderEagI

    Close

    OrderEagI-HF®

    Close

    OrderEarI

    Close

    OrderEco53kI

    Close

    OrderEcoNI

    Close

    OrderEcoO109I

    Close

    OrderEcoP15I

    Close

    OrderEcoRI

    Close

    OrderEcoRI-HF®

    Close

    OrderEcoRI-HF® RE-Mix®

    Close

    OrderEcoRV

    Close

    OrderEcoRV-HF®

    Close

    OrderEcoRV-HF® RE-Mix®

    Close

    OrderFnu4HI

    Close

    OrderFseI

    Close

    OrderFspI

    Close

    OrderHaeIII

    Close

    OrderHhaI

    Close

    OrderHincII

    Close

    OrderHindIII-HF®

    Close

    OrderHinfI

    Close

    OrderHinP1I

    Close

    OrderHpaII

    Close

    OrderHphI

    Close

    OrderHpy166II

    Close

    OrderHpyAV

    Close

    OrderHpyCH4IV

    Close

    OrderHpyCH4V

    Close

    OrderKpnI

    Close

    OrderKpnI-HF®

    Close

    OrderKpnI-HF® RE-Mix®

    Close

    OrderMboI

    Close

    OrderMfeI-HF®

    Close

    OrderMfeI-HF® RE-Mix®

    Close

    OrderMluCI

    Close

    OrderMluI

    Close

    OrderMlyI

    Close

    OrderMmeI

    Close

    OrderMnlI

    Close

    OrderMseI

    Close

    OrderMslI

    Close

    OrderMspA1I

    Close

    OrderMspI

    Close

    OrderMwoI

    Close

    OrderNciI

    Close

    OrderNcoI

    Close

    OrderNcoI-HF®

    Close

    OrderNcoI-HF® RE-Mix®

    Close

    OrderNdeI

    Close

    OrderNgoMIV

    Close

    OrderNheI

    Close

    OrderNheI-HF®

    Close

    OrderNheI-HF® RE-Mix®

    Close

    OrderNlaIII

    Close

    OrderNotI

    Close

    OrderNotI-HF®

    Close

    OrderNotI-HF® RE-Mix®

    Close

    OrderNruI

    Close

    OrderNsiI

    Close

    OrderNspI

    Close

    OrderPacI

    Close

    OrderPacI RE-Mix®

    Close

    OrderPaeR7I

    Close

    OrderPflFI

    Close

    OrderPflMI

    Close

    OrderPmeI

    Close

    OrderPmlI

    Close

    OrderPpuMI

    Close

    OrderPshAI

    Close

    OrderPstI

    Close

    OrderPstI-HF®

    Close

    OrderPvuI

    Close

    OrderPvuI-HF®

    Close

    OrderPvuII

    Close

    OrderPvuII-HF®

    Close

    OrderRsaI

    Close

    OrderSacI

    Close

    OrderSacI-HF®

    Close

    OrderSacII

    Close

    OrderSalI

    Close

    OrderSalI-HF®

    Close

    OrderSalI-HF® RE-Mix®

    Close

    OrderSapI

    Close

    OrderSbfI

    Close

    OrderSbfI-HF®

    Close

    OrderScaI-HF®

    Close

    OrderScaI-HF® RE-Mix®

    Close

    OrderSfiI

    Close

    OrderSfoI

    Close

    OrderSmaI

    Close

    OrderSpeI

    Close

    OrderSpeI RE-Mix®

    Close

    OrderSpeI-HF®

    Close

    OrderSphI-HF®

    Close

    OrderSspI-HF®

    Close

    OrderStuI

    Close

    OrderStyD4I

    Close

    OrderStyI

    Close

    OrderStyI-HF®

    Close

    OrderSwaI

    Close

    OrderTaqαI

    Close

    OrderTfil

    Close

    OrderTseI

    Close

    OrderTspMI

    Close

    OrderTspRI

    Close

    OrderTth111I

    Close

    OrderXbaI

    Close

    OrderXbaI RE-Mix®

    Close

    OrderXhoI

    Close

    OrderXhoI RE-Mix®

    Close

    OrderXmaI

    Close

    OrderXmnI

    High-Fidelity (HF®) Restriction Endonucleases

    Close

    OrderAgeI-HF®

    Close

    OrderAgeI-HF® RE-Mix®

    Close

    OrderBamHI-HF®

    Close

    OrderBmtI-HF®

    Close

    OrderBsaI-HF®

    Close

    OrderBstEII-HF®

    Close

    OrderBstEII-HF® RE-Mix®

    Close

    OrderDraIII-HF®

    Close

    OrderEagI-HF®

    Close

    OrderEcoRI-HF®

    Close

    OrderEcoRI-HF® RE-Mix®

    Close

    OrderEcoRV-HF®

    Close

    OrderEcoRV-HF® RE-Mix®

    Close

    OrderHindIII-HF®

    Close

    OrderKpnI-HF®

    Close

    OrderKpnI-HF® RE-Mix®

    Close

    OrderMfeI-HF®

    Close

    OrderMfeI-HF® RE-Mix®

    Close

    OrderNcoI-HF®

    Close

    OrderNcoI-HF® RE-Mix®

    Close

    OrderNheI-HF®

    Close

    OrderNheI-HF® RE-Mix®

    Close

    OrderNotI-HF®

    Close

    OrderNotI-HF® RE-Mix®

    Close

    OrderPstI-HF®

    Close

    OrderPvuI-HF®

    Close

    OrderPvuII-HF®

    Close

    OrderSacI-HF®

    Close

    OrderSalI-HF®

    Close

    OrderSalI-HF® RE-Mix®

    Close

    OrderSbfI-HF®

    Close

    OrderScaI-HF®

    Close

    OrderScaI-HF® RE-Mix®

    Close

    OrderSpeI-HF®

    Close

    OrderSphI-HF®

    Close

    OrderSspI-HF®

    Close

    OrderStyI-HF®

    Homing Endonucleases

    Close

    OrderI-CeuI

    Close

    OrderI-SceI

    Close

    OrderPI-PspI

    Close

    OrderPI-SceI

    Nicking Endonucleases

    Close

    OrderNb.BbvCI

    Close

    OrderNb.BsmI

    Close

    OrderNb.BsrDI

    Close

    OrderNb.BtsI

    Close

    OrderNt.AlwI

    Close

    OrderNt.BbvCI

    Close

    OrderNt.BsmAI

    Close

    OrderNt.BspQI

    Close

    OrderNt.BstNBI

    Close

    OrderNt.CviPII

    RE-Mix® Restriction Enzyme Master Mixes

    Close

    OrderAgeI-HF® RE-Mix®

    Close

    OrderAscI RE-Mix®

    Close

    OrderBstEII-HF® RE-Mix®

    Close

    OrderEcoRI-HF® RE-Mix®

    Close

    OrderEcoRV-HF® RE-Mix®

    Close

    OrderKpnI-HF® RE-Mix®

    Close

    OrderMfeI-HF® RE-Mix®

    Close

    OrderNcoI-HF® RE-Mix®

    Close

    OrderNheI-HF® RE-Mix®

    Close

    OrderNotI-HF® RE-Mix®

    Close

    OrderPacI RE-Mix®

    Close

    OrderSalI-HF® RE-Mix®

    Close

    OrderScaI-HF® RE-Mix®

    Close

    OrderSpeI RE-Mix®

    Close

    OrderXbaI RE-Mix®

    Close

    OrderXhoI RE-Mix®

    Restriction Enzymes for Epigenetic Analysis (EpiMark® Validated)

    Close

    OrderDpnI

    Close

    OrderDpnII

    Close

    OrderFspEI

    Close

    OrderHpaII

    Close

    OrderLpnPI

    Close

    OrderMcrBC

    Close

    OrderMspI

    Close

    OrderMspJI

    Time-Saver™ Qualified Restriction Enzymes

    Close

    OrderAatII

    Close

    OrderAcc65I

    Close

    OrderAccI

    Close

    OrderAciI

    Close

    OrderAclI

    Close

    OrderAcuI

    Close

    OrderAflII

    Close

    OrderAgeI-HF®

    Close

    OrderAgeI-HF® RE-Mix®

    Close

    OrderAhdI

    Close

    OrderAluI

    Close

    OrderAlwNI

    Close

    OrderApaI

    Close

    OrderApaLI

    Close

    OrderApeKI

    Close

    OrderApoI

    Close

    OrderAscI

    Close

    OrderAscI RE-Mix®

    Close

    OrderAseI

    Close

    OrderAvaI

    Close

    OrderAvaII

    Close

    OrderAvrII

    Close

    OrderBaeGI

    Close

    OrderBaeI

    Close

    OrderBamHI

    Close

    OrderBamHI-HF®

    Close

    OrderBbsI

    Close

    OrderBbvI

    Close

    OrderBciVI

    Close

    OrderBclI

    Close

    OrderBcoDI

    Close

    OrderBfuAI

    Close

    OrderBfuCI

    Close

    OrderBglI

    Close

    OrderBglII

    Close

    OrderBlpI

    Close

    OrderBmgBI

    Close

    OrderBmtI-HF®

    Close

    OrderBpmI

    Close

    OrderBpuEI

    Close

    OrderBsaAI

    Close

    OrderBsaHI

    Close

    OrderBsaI-HF®

    Close

    OrderBsaWI

    Close

    OrderBsaXI

    Close

    OrderBseRI

    Close

    OrderBsgI

    Close

    OrderBsiEI

    Close

    OrderBsiWI

    Close

    OrderBslI

    Close

    OrderBsmAI

    Close

    OrderBsmBI

    Close

    OrderBsmI

    Close

    OrderBsoBI

    Close

    OrderBsp1286I

    Close

    OrderBspCNI

    Close

    OrderBspEI

    Close

    OrderBspHI

    Close

    OrderBspQI

    Close

    OrderBsrBI

    Close

    OrderBsrDI

    Close

    OrderBsrGI

    Close

    OrderBsrI

    Close

    OrderBssHII

    Close

    OrderBssKI

    Close

    OrderBstBI

    Close

    OrderBstEII

    Close

    OrderBstEII-HF®

    Close

    OrderBstEII-HF® RE-Mix®

    Close

    OrderBstNI

    Close

    OrderBstUI

    Close

    OrderBstXI

    Close

    OrderBstYI

    Close

    OrderBstZ17I

    Close

    OrderBsu36I

    Close

    OrderBtgI

    Close

    OrderBtsCI

    Close

    OrderCac8I

    Close

    OrderClaI

    Close

    OrderCspCI

    Close

    OrderCviAII

    Close

    OrderCviQI

    Close

    OrderDdeI

    Close

    OrderDpnI

    Close

    OrderDpnII

    Close

    OrderDraI

    Close

    OrderDraIII-HF®

    Close

    OrderDrdI

    Close

    OrderEagI

    Close

    OrderEagI-HF®

    Close

    OrderEarI

    Close

    OrderEco53kI

    Close

    OrderEcoNI

    Close

    OrderEcoO109I

    Close

    OrderEcoP15I

    Close

    OrderEcoRI

    Close

    OrderEcoRI-HF®

    Close

    OrderEcoRI-HF® RE-Mix®

    Close

    OrderEcoRV

    Close

    OrderEcoRV-HF®

    Close

    OrderEcoRV-HF® RE-Mix®

    Close

    OrderFnu4HI

    Close

    OrderFseI

    Close

    OrderFspI

    Close

    OrderHaeIII

    Close

    OrderHhaI

    Close

    OrderHincII

    Close

    OrderHindIII-HF®

    Close

    OrderHinfI

    Close

    OrderHinP1I

    Close

    OrderHpaII

    Close

    OrderHphI

    Close

    OrderHpy166II

    Close

    OrderHpyAV

    Close

    OrderHpyCH4IV

    Close

    OrderHpyCH4V

    Close

    OrderKpnI

    Close

    OrderKpnI-HF®

    Close

    OrderKpnI-HF® RE-Mix®

    Close

    OrderMboI

    Close

    OrderMfeI-HF®

    Close

    OrderMfeI-HF® RE-Mix®

    Close

    OrderMluCI

    Close

    OrderMluI

    Close

    OrderMlyI

    Close

    OrderMmeI

    Close

    OrderMnlI

    Close

    OrderMseI

    Close

    OrderMslI

    Close

    OrderMspA1I

    Close

    OrderMspI

    Close

    OrderMwoI

    Close

    OrderNciI

    Close

    OrderNcoI

    Close

    OrderNcoI-HF®

    Close

    OrderNcoI-HF® RE-Mix®

    Close

    OrderNdeI

    Close

    OrderNgoMIV

    Close

    OrderNheI

    Close

    OrderNheI-HF®

    Close

    OrderNheI-HF® RE-Mix®

    Close

    OrderNlaIII

    Close

    OrderNotI

    Close

    OrderNotI-HF®

    Close

    OrderNotI-HF® RE-Mix®

    Close

    OrderNruI

    Close

    OrderNsiI

    Close

    OrderNspI

    Close

    OrderPacI

    Close

    OrderPacI RE-Mix®

    Close

    OrderPaeR7I

    Close

    OrderPflFI

    Close

    OrderPflMI

    Close

    OrderPmeI

    Close

    OrderPmlI

    Close

    OrderPpuMI

    Close

    OrderPshAI

    Close

    OrderPstI

    Close

    OrderPstI-HF®

    Close

    OrderPvuI

    Close

    OrderPvuI-HF®

    Close

    OrderPvuII

    Close

    OrderPvuII-HF®

    Close

    OrderRsaI

    Close

    OrderSacI

    Close

    OrderSacI-HF®

    Close

    OrderSacII

    Close

    OrderSalI

    Close

    OrderSalI-HF®

    Close

    OrderSalI-HF® RE-Mix®

    Close

    OrderSapI

    Close

    OrderSbfI

    Close

    OrderSbfI-HF®

    Close

    OrderScaI-HF®

    Close

    OrderScaI-HF® RE-Mix®

    Close

    OrderSfiI

    Close

    OrderSfoI

    Close

    OrderSmaI

    Close

    OrderSpeI

    Close

    OrderSpeI RE-Mix®

    Close

    OrderSpeI-HF®

    Close

    OrderSphI-HF®

    Close

    OrderSspI-HF®

    Close

    OrderStuI

    Close

    OrderStyD4I

    Close

    OrderStyI

    Close

    OrderStyI-HF®

    Close

    OrderSwaI

    Close

    OrderTaqαI

    Close

    OrderTfil

    Close

    OrderTseI

    Close

    OrderTspMI

    Close

    OrderTspRI

    Close

    OrderTth111I

    Close

    OrderXbaI

    Close

    OrderXbaI RE-Mix®

    Close

    OrderXhoI

    Close

    OrderXhoI RE-Mix®

    Close

    OrderXmaI

    Close

    OrderXmnI

    Can't find what you need?