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  • Fetuin

    Description

    Fetuin is a glycoprotein containing sialylated N-linked and O-linked glycans that can be used as a positive control for endoglycosidase enzymes.

    Product Source

    Fetal Calf Serum

    Properties and Usage

    Storage Temperature

    4°C

    Storage Conditions

    1X Milli-Q water

    Molecular Weight

    Apparent: 64 kDa

    Notes

    1. 500 μg is enough for approximately 20 reactions.
    2. Due to heterogeneous glycosylation; Fetuin runs as a doublet on an SDS-PAGE gel.

    FAQs

    1. What is a good endoglycosidase control substrate?
    2. Why is Fetuin a good control substrate for both O-Glycosidase, PNGase F and the Protein Deglycosylation Mix?
    3. What is a typical Fetuin deglycosylation reaction condition?
    4. Do detergents inhibit exo- and endoglycosidases?
    5. How much endoglycosidase should I use to deglycosylate Fetuin under native conditions?

    Datacards

    The Product Summary Sheet, or Data Card, includes details for how to use the product, as well as details of its formulation and quality controls. The following file naming structure is used to name the majority of these document files: [Catalog Number]Datasheet-Lot[Lot Number]. For those product lots not listed below, please contact NEB at info@neb.com or fill out the Technical Support Form for appropriate document.

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    Safety Data Sheet

    The following is a list of Safety Data Sheet (SDS) that apply to this product to help you use it safely.

    Datacards

    The Product Summary Sheet, or Data Card, includes details for how to use the product, as well as details of its formulation and quality controls. The following file naming structure is used to name the majority of these document files: [Catalog Number]Datasheet-Lot[Lot Number]. For those product lots not listed below, please contact NEB at info@neb.com or fill out the Technical Support Form for appropriate document.