100 bp DNA Ladder

The following reagents are supplied with this product:

NEB #	Component Name	Component #	Stored at (°C)	Amount	Concentration
N3231S     -20       100 bp DNA Ladder N3231SVIAL -20 1 x 0.1 ml 500 µg/ml   Gel Loading Dye, Purple (6X), no SDS B7025SVIAL 25 1 x 1 ml 6 X
N3231L     -20       100 bp DNA Ladder N3231LVIAL -20 1 x 0.5 ml 500 µg/ml   Gel Loading Dye, Purple (6X), no SDS B7025SVIAL 25 1 x 1 ml 6 X

Companion Products

• 1 kb DNA Ladder
• TriDye™ 1 kb Plus DNA Ladder
• TriDye™ 100 bp DNA Ladder
• TriDye™ 1 kb DNA Ladder
• Quick-Load^® 1 kb Plus DNA Ladder
• Quick-Load® 100 bp DNA Ladder
• Quick-Load® 1 kb DNA Ladder
• PCR Marker
• 50 bp DNA Ladder
• Gel Loading Dye, Purple (6X), no SDS
• Monarch® Plasmid Miniprep Kit 
• Monarch® DNA Gel Extraction Kit
• Monarch® PCR & DNA Cleanup Kit (5 μg)

Materials Sold Separately

• Gel Loading Dye, Purple (6X), no SDS

1. This ladder was not designed for precise quantification of DNA mass but can be used for approximating the mass of DNA in comparably intense samples of similar size.
2. We recommend loading 0.5 μg of 100 bp DNA Ladder diluted in sample buffer.
3. All fragments have 4-base, 5´ overhangs that can be end labeled using T4 Polynucleotide Kinase (#M0201) or filled-in using DNA Polymerase I, Klenow Fragment (#M0210) (1). Use α-[³²P] dATP or α-[³²P] dTTP for the fill-in reaction.
4. 100 bp DNA Ladder is stable for at least 3 months at 4°C.
5. For long term storage store at -20°C. If samples need to be diluted, use TE or other buffer of minimal ionic strength. DNA may denature if diluted in dH₂O.
6. Due to the limitations of the acrylamide gel technology, one or two extra bands may be visible on the DNA ladders when run on a polyacrylamide gel.
7. 1X Gel Loading Dye, Purple (6X), no SDS:
   2.5% Ficoll®-400 
   10mM EDTA 
   3.3mM Tris-HCl (pH 8.0@25°C)
   0.02% Dye 1 
   0.001% Dye 2 

1. Sambrook, J., Fritsch, E.F. and Maniatis, T. (1989). Molecular Cloning: A Laboratory Manual (2nd Ed.). 10.51-10.67.

1. End-labeling Protocol
2. Suggested Loading Protocol for DNA Ladders & Markers

• DNA Markers & Ladders Selection Tool

1. Why are there extra bands visible on polyacrylamide gels?
2. Why is the separation of the lower bands incomplete?
3. What are the overhangs on the DNA ladder fragments? Can I end-label them using the T4 polynucleotide kinase (PNK)? What about the Klenow fragment?
4. Why are the DNA ladders showing up on my Southern blot? What is the sequence or composition of the ladder bands?
5. How can I quantify the amount of DNA in each band of a marker?
6. Can I use Midori Green with the DNA Ladders from NEB?
7. Can I use SYBR^® and/or GelRed^® dyes with the DNA Ladders from NEB?
8. Why is my DNA Ladder floating out the wells and Why are there no bands visible in my DNA ladder gel lane?
9. What are the differences in the four versions of 2-Log, 100 bp and 1 kb DNA Ladders that NEB sells?

Quality Control tests are performed on each new lot of NEB product to meet the specifications designated for it. Specifications and individual lot data from the tests that are performed for this particular product can be found and downloaded on the Product Specification Sheet, Certificate of Analysis, data card or product manual. Further information regarding NEB product quality can be found here.

The Specification sheet is a document that includes the storage temperature, shelf life and the specifications designated for the product. The following file naming structure is used to name these document files: [Product Number]_[Size]_[Version]

• N3231S_L_v1

The Certificate of Analysis (COA) is a signed document that includes the storage temperature, expiration date and quality controls for an individual lot. The following file naming structure is used to name these document files: [Product Number]_[Size]_[Version]_[Lot Number]

• N3231S_L_v1_1021401
• N3231S_L_v1_1031404
• N3231S_L_v1_1041407
• N3231S_L_v1_1051409
• N3231S_L_v1_1071501
• N3231S_L_v1_1061411
• N3231S_L_v1_1081504
• N3231S_L_v1_1091506
• N3231S_L_v1_1101509
• N3231S_L_v1_1111511
• N3231S_L_v1_1121601
• N3231S_L_v1_1131604
• N3231S_L_v1_1141605
• N3231S_L_v1_1151607
• N3231S_L_v1_1161611
• N3231S_L_v1_1171703
• N3231S_L_v1_1181704
• N3231S_L_v1_1201709
• N3231S_L_v1_1211712
• N3231S_L_v1_1231804
• N3231S_v1_10012403
• N3231L_v1_10017498
• N3231S_v1_10016045
• N3231S_v1_10017499
• N3231S_v1_10020641
• N3231S_v1_10023167
• N3231S_v1_10025274
• N3231L_v1_10025581
• N3231S_v1_10031376
• N3231L_v1_10033095
• N3231S_v1_10036135
• N3231S_v1_10033097
• N3231S_v1_10039027
• N3231L_v1_10040243
• N3231S_v1_10042141
• N3231S_v1_10045568
• N3231L_v1_10047325
• N3231S_v1_10047326
• N3231S_v1_10050211
• N3231L_v1_10051052
• N3231S_v1_10052811
• N3231S_v1_10055055
• N3231L_v1_10055533
• N3231S_v1_10057990
• N3231L_v1_10059499
• N3231S_v1_10060881
• N3231L_v1_10063455
• N3231S_v1_10064232
• N3231S_v1_10068674
• N3231L_v1_10069716
• N3231S_v1_10072221
• N3231L_v1_10076923
• N3231S_v1_10079889
• N3231L_v1_10080699
• N3231S_v1_10082394
• N3231L_v1_10084436
• N3231S_v1_10087053
• N3231L_v1_10090969
• N3231S_v1_10092202
• N3231L_v1_10095218
• N3231S_v1_10095220
• N3231S_v1_10101087
• N3231S_v1_10105328
• N3231L_v1_10107006
• N3231S_v1_10110871
• N3231S_v1_10114815
• N3231L_v1_10119693
• N3231S_v1_10121106
• N3231S_v1_10126558
• N3231L_v1_10126557
• N3231S_v1_10132757
• N3231L_v1_10137531
• N3231L_v1_10143516
• N3231S_v1_10139922
• N3231S_v1_10145514
• N3231L_v1_10152195
• N3231S_v1_10154666
• N3231L_v1_10160604
• N3231S_v1_10160603
• N3231L_v1_10167489
• N3231S_v1_10167490
• N3231S_v1_10175787
• N3231L_v1_10180927
• N3231S_v1_10180926
• N3231S_v1_10187147
• N3231L_v1_10187146
• N3231S_v1_10199296
• N3231L_v1_10195943
• N3231S_v1_10203709
• N3231L_v1_10203710
• N3231S_v1_10215042
• N3231L_v1_10222379
• N3231S_v1_10222008
• N3231S_v1_10222380
• N3231L_v1_10231516
• N3231S_v1_10235834
• N3231S_v1_10243966
• N3231L_v1_10243987
• N3231S_v1_10249648
• N3231L_v1_10249649
• N3231S_v1_10255999

The following is a list of Safety Data Sheet (SDS) that apply to this product to help you use it safely.

• 100 bp DNA Ladder
• Gel Loading Dye, Purple (6X), no SDS

Products and content are covered by one or more patents, trademarks and/or copyrights owned or controlled by New England Biolabs, Inc (NEB). The use of trademark symbols does not necessarily indicate that the name is trademarked in the country where it is being read; it indicates where the content was originally developed. The use of this product may require the buyer to obtain additional third-party intellectual property rights for certain applications. For more information, please email busdev@neb.com.

This product is intended for research purposes only. This product is not intended to be used for therapeutic or diagnostic purposes in humans or animals.

New England Biolabs (NEB) is committed to practicing ethical science – we believe it is our job as researchers to ask the important questions that when answered will help preserve our quality of life and the world that we live in. However, this research should always be done in safe and ethical manner. Learn more.