OneTaq® Quick-Load® DNA Polymerase
The ONE polymerase for your endpoint PCR needs
OneTaq DNA Polymerase is an optimized blend of Taq and Deep Vent™ DNA polymerases for use with routine and difficult PCR experiments.
- Includes green Quick-Load reaction buffer for easy and direct loading of PCR products onto gels
- 2X higher fidelity than Taq
- Compatible with standard Taq protocols
OneTaq® Quick-Load® DNA Polymerase is an optimized, ready-to-use blend of Taq and Deep Vent™ DNA Polymerases ideally suited to routine PCR applications from a variety of templates, including pure DNA solutions, bacterial colonies and cDNA products. The 3´→5´ exonuclease activity of Deep Vent DNA Polymerase increases the fidelity and robust amplification of Taq DNA Polymerase (1). The convenient quick-load buffer contains two commonly used tracking dyes for direct loading of PCR products on DNA gels. On a 1% agarose gel in 1X TBE, Xylene Cyanol FF migrates at ~4 kb and Tartrazine migrates at ~10 bp. Both dyes are present in concentrations that do not mask comigrating DNA bands.
Product SourceAn E. coli strain that carries the Taq DNA Polymerase gene from Thermus aquaticus YT-1 and an E. coli strain that carries the Deep VentR DNA Polymerase gene from Pyrococcus species GB-D.
The following reagents are supplied with this product:
Store at (°C) Concentration OneTaq® Standard Reaction Buffer Pack -20 5 X
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