OneTaq® Quick-Load® 2X Master Mix with Standard Buffer
The ONE polymerase for your endpoint PCR needs
OneTaq DNA Polymerase is an optimized blend of Taq and Deep Vent® DNA polymerases for use with routine and difficult PCR experiments.
- Obtain high yields across a wide range of AT- and GC- content
- 2X higher fidelity than Taq
- Master mix format contains inert tracking dye for easy and direct loading of PCR products onto gels
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Overview of PCR
OneTaq® Quick-Load® 2X Master Mix with Standard Buffer is an optimized, ready-to-use blend of Taq and Deep VentR ™ DNA Polymerases ideally suited to routine PCR applications from a variety of templates, including pure DNA solutions, bacterial colonies and cDNA products. The 3′→5′ exonuclease activity of Deep Vent DNA Polymerase increases the fidelity and robust amplification of Taq DNA Polymerase (1). The convenient quick-load master mix formulation contains dNTPs, MgCl2, buffer components and stabilizers as well as two commonly used tracking dyes for DNA gels. On a 1% agarose gel in 1X TBE, Xylene Cyanol FF migrates at ~4 kb and Tartrazine migrates at ~10 bp. Both dyes are present in concentrations that do not mask comigrating DNA bands.
Product SourceAn E. coli strain that carries the Taq DNA Polymerase gene from Thermus aquaticus YT-1 and an E. coli strain that carries the Deep VentR DNA Polymerase gene from Pyrococcus species GB-D.
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