OneTaq^® Hot Start 2X Master Mix with Standard Buffer

The following reagents are supplied with this product:

NEB #	Component Name	Component #	Stored at (°C)	Amount	Concentration
M0484S     -20       OneTaq® Hot Start 2X Master Mix with Standard Buffer M0484SVIAL -20 2 x 1.25 ml 2 X
M0484L     -20       OneTaq® Hot Start 2X Master Mix with Standard Buffer M0484SVIAL -20 10 x 1.25 ml 2 X

Application Features

• High Throughput PCR
• High Sensitivity PCR
• Routine PCR
• AT-rich PCR
• Colony PCR
• Long PCR (up to ~6 kb genomic)

Companion Products

• Magnesium Chloride (MgCl₂) Solution
• OneTaq® DNA Polymerase
• OneTaq® Hot Start 2X Master Mix with GC Buffer
• OneTaq® Hot Start DNA Polymerase
• OneTaq^® Quick-Load^® 2X Master Mix with Standard Buffer
• OneTaq® Hot Start Quick-Load® 2X Master Mix with GC Buffer
• OneTaq® 2X Master Mix with Standard Buffer

1. Product specifications for individual components in the OneTaq Hot Start 2X Master Mix with Standard Buffer are available separately.
2. OneTaq Hot Start 2X Master Mix with Standard Buffer is stable for fifteen freeze-thaw cycles when stored at -20°C.
3. OneTaq Hot Start 2X Master Mix with Standard Buffer is also stable for one month at 4°C, so for frequent use, an aliquot may be kept at 4°C.

1. Barnes, W.M (1994). Proc.Natl.Acad.Sci.USA. 91, 2216-2220.
2. Saiki, R.K. et al. (1994). Science. 91, 2216-2220.
3. Powell, L.M. et al. (1987). Cell. 50, 831-840.
4. Sun, Y, Hegamyer, G. and Colburn, N. (1993). Biotechniques. 15, 372-374.

1. Protocol for OneTaq® Hot Start 2X Master Mix with Standard Buffer (M0484)

• Activity of Restriction Enzymes in PCR Buffers
• Guidelines for PCR Optimization with OneTaq® and OneTaq® Hot Start DNA Polymerases
• Guidelines for PCR Optimization with Thermophilic DNA Polymerases

• Robust Colony PCR from Multiple E. coli Strains using OneTaq^® Quick-Load^® Master Mixes

• DNA Polymerase Selection Chart
• Thermophilic DNA Polymerases

• PCR Selector
• Tm Calculator

1. What ends will my PCR products have?
2. What are Hot Start and WarmStart^® polymerases and when would I use them?
3. How should I determine the appropriate annealing temperature for my reaction?
4. What should the final primer concentration be in my PCR?
5. What are the properties of this polymerase (fidelity, product ends, max amplicon, modified base incorporation, etc.)?
6. What are the stability and storage requirements for NEB's Taq and OneTaq DNA Polymerases?
7. Can Taq/OneTaq^® DNA Polymerases be used for nick translation?
8. How can I optimize my PCR when using Taq DNA Polymerase?

• PCR Troubleshooting Guide

• Using aptamers to control enzyme activities: Hot Start Taq and beyond

Quality Control tests are performed on each new lot of NEB product to meet the specifications designated for it. Specifications and individual lot data from the tests that are performed for this particular product can be found and downloaded on the Product Specification Sheet, Certificate of Analysis, data card or product manual. Further information regarding NEB product quality can be found here.

The Specification sheet is a document that includes the storage temperature, shelf life and the specifications designated for the product. The following file naming structure is used to name these document files: [Product Number]_[Size]_[Version]

• M0484S_L_v1
• M0484S_L_v2

The Certificate of Analysis (COA) is a signed document that includes the storage temperature, expiration date and quality controls for an individual lot. The following file naming structure is used to name these document files: [Product Number]_[Size]_[Version]_[Lot Number]

• M0484S_L_v1_0211611
• M0484S_L_v1_0221706
• M0484S_L_v1_0231712
• M0484S_v1_10010143
• M0484L_v1_10017144
• M0484S_v1_10026527
• M0484L_v1_10032454
• M0484L_v1_10033858
• M0484S_v1_10037200
• M0484L_v1_10037197
• M0484L_v1_10044962
• M0484S_v1_10042651
• M0484S_v1_10046507
• M0484L_v1_10048704
• M0484S_v1_10050137
• M0484L_v1_10053917
• M0484S_v1_10055863
• M0484L_v1_10056305
• M0484L_v1_10061959
• M0484S_v1_10059570
• M0484L_v1_10063544
• M0484S_v1_10063543
• M0484L_v2_10068699
• M0484L_v2_10073570
• M0484S_v2_10079196
• M0484L_v2_10082260
• M0484L_v2_10085963
• M0484S_v2_10083679
• M0484L_v2_10088151
• M0484L_v2_10093277
• M0484L_v2_10097530
• M0484S_v2_10097450
• M0484S_v2_10103960
• M0484L_v2_10104391
• M0484L_v2_10109419
• M0484S_v2_10108198
• M0484L_v2_10110686
• M0484S_v2_10113509
• M0484L_v2_10115039
• M0484L_v2_10120940
• M0484L_v2_10123021
• M0484S_v2_10122823
• M0484L_v2_10126061
• M0484S_v2_10129102
• M0484L_v2_10137188
• M0484S_v2_10140686
• M0484S_v2_10144495
• M0484L_v2_10144496
• M0484S_v2_10146130
• M0484S_v2_10151893
• M0484L_v2_10151382
• M0484S_v2_10156553
• M0484L_v2_10160177
• M0484S_v2_10162257
• M0484L_v2_10164441
• M0484S_v2_10165618
• M0484S_v2_10174283
• M0484L_v2_10172083
• M0484S_v2_10179242
• M0484L_v2_10181978
• M0484L_v2_10193692
• M0484S_v2_10191849
• M0484L_v2_10203657
• M0484S_v2_10202782
• M0484S_v2_10209408
• M0484L_v2_10212504
• M0484S_v2_10221156
• M0484L_v2_10221155
• M0484S_v2_10231830
• M0484L_v2_10236854
• M0484L_v2_10246702
• M0484S_v2_10246700
• M0484L_v2_10252450
• M0484S_v2_10249375
• M0484L_v2_10259112

The following is a list of Safety Data Sheet (SDS) that apply to this product to help you use it safely.

• OneTaq^® Hot Start 2X Master Mix with Standard Buffer

Products and content are covered by one or more patents, trademarks and/or copyrights owned or controlled by New England Biolabs, Inc (NEB). The use of trademark symbols does not necessarily indicate that the name is trademarked in the country where it is being read; it indicates where the content was originally developed. The use of this product may require the buyer to obtain additional third-party intellectual property rights for certain applications. For more information, please email busdev@neb.com.

This product is intended for research purposes only. This product is not intended to be used for therapeutic or diagnostic purposes in humans or animals.

New England Biolabs (NEB) is committed to practicing ethical science – we believe it is our job as researchers to ask the important questions that when answered will help preserve our quality of life and the world that we live in. However, this research should always be done in safe and ethical manner. Learn more.

Licenses

Nucleic acid-based aptamers for use with thermophilic DNA polymerases are licensed exclusively by New England Biolabs, Inc. from SomaLogic, Inc. New England Biolabs, Inc. gives the Buyer/User a non-exclusive license to use the OneTaq® Hot Start 2X Master Mix with Standard Buffer for Research Use Only (RUO). Commercial use of this product may require a license from New England Biolabs, Inc. For additional information or to inquire about commercial use, please contact busdev@neb.com.

 

Trademarks

NEW ENGLAND BIOLABS^®, ONETAQ^®, QUICK-LOAD^® and THERMOPOL^® are registered trademarks of New England Biolabs, Inc. 
DEEPVENT^® is a trademark of New England Biolabs, Inc. 

IGEPAL^® is a registered trademark of Rhodia Operations.
TWEEN^® is a registered trademark of Uniqema Americas LLC.