OneTaq® Hot Start 2X Master Mix with Standard Buffer
OneTaq® DNA Polymerase is an optimized blend of Taq and Deep Vent™ DNA polymerases for use with routine and difficult PCR experiments.
- Obtain high yields across a wide range of AT / GC content
- 2X higher fidelity than Taq
- Hot Start feature enables room temperature reaction setup
- Master mix is a 2X concentrated solution containing everything needed for robust amplification
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OneTaq® Hot Start 2X Master Mix with Standard Buffer is an optimized blend of Taq and Deep VentR ™ DNA Polymerases combined with an aptamer-based inhibitor. This enzyme blend is ideally suited to routine PCR applications from a variety of templates, including pure DNA solutions, bacterial colonies and cDNA products. The 3´→5´ exonuclease activity of Deep Vent DNA Polymerase increases the fidelity and robust amplification of Taq DNA Polymerase (1). The hot start nature of the enzyme offers convenience with decreased interference from primer dimers and secondary products. The convenient master mix formulation contains dNTPs, MgCl2 and other buffer components and stabilizers listed below, requiring only the addition of primers and DNA template for robust amplification
Product SourceAn E. coli strain that carries the Taq DNA Polymerase gene from Thermus aquaticus YT-1 and an E. coli strain that carries the Deep VentR DNA Polymerase gene from Pyrococcus species GB-D.
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