T4 RNA Ligase 1 (ssRNA Ligase), High Concentration
Avoiding RNase Contamination
T4 RNA Ligase 1, High Concentration (M0437M), is a 3X higher concentration than our standard T4 RNA Ligase 1 (M0204S/L). This product is packaged with a vial of 100 mM ATP (N0451A). The increased concentration of reaction components is helpful in lower volume reactions where reagent volumes are limited.
T4 RNA Ligase 1 catalyzes the ligation of a 5´ phosphoryl-terminated nucleic acid donor to a 3´ hydroxyl-terminated nucleic acceptor through the formation of a 3´ → 5´ phosphodiester bond, with hydrolysis of ATP to AMP and PPi. Substrates include singlestranded RNA and DNA as well as dinucleoside pyrophosphates (1).
Product SourceAn E. coli strain that carries the T4 RNA Ligase 1 gene
The following reagents are supplied with this product:
Store at (°C) Concentration T4 RNA Ligase Reaction Buffer -20 10 X Adenosine-5'-Triphosphate (ATP) -20 100 mM PEG 8000 -20 50 %
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