WarmStart® RTx Reverse Transcriptase
- Ideal for use in Loop-Mediated Isothermal Amplification (LAMP))
- WarmStart® feature enables high-throughput applications, room temperature reaction setup, and increases the consistency and specificity of amplification reactions
Avoiding RNase Contamination
WarmStart RTx Reverse Transcriptase is a unique in silico designed RNA-directed DNA polymerase coupled with a reversibly-bound aptamer that inhibits RTx activity below 40°C. This enzyme can synthesize a complementary DNA strand initiating from a primer using RNA (cDNA synthesis) or single-stranded DNA as a template. RTx is a robust enzyme for RNA detection in amplification reactions and is particularly well suited for use in LAMP (loop-mediated isothermal amplification). The WarmStart property enables high throughput applications, room temperature setup, and increases the consistency and specificity of amplification reactions. RTx contains intact RNase H activity.
Sensitive Detection with Reduced False Negatives
Total RNA WarmStart RTx AMV RT 100 ng 3/3 3/3 10 ng 3/3 3/3 1 ng 3/3 3/3 100 pg 3/3 2/3 10 pg 3/3 2/3 1pg 3/3 1/3
RT-LAMP reactions were performed with WarmStart RTx and AMV Reverse Transcriptase with total RNA at 6 input concentrations. Positive detection, indicated by fluorescent detection with a twenty-minute reaction time, was achieved down to 1 pg of total input RNA. Use of AMV resulted in false negatives when total RNA was 100 pg or lower.
The following reagents are supplied with this product:
Store at (°C) Concentration Isothermal Amplification Buffer Pack -20 10X Magnesium Sulfate (MgSO4) Solution -20 100 mM
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