ElectroLigase® combines T4 DNA Ligase and an optimized, ready-to-use 2X reaction buffer containing a proprietary ligation enhancer and no PEG. This combination is specifically formulated to promote robust ligation of all types of DNA ends (e.g., blunt, sticky, TA). It is directly compatible with electrocompetent cells used for transformation by electroporation, without desalting or purification.
The following reagents are supplied with this product:
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