ElectroLigase® combines T4 DNA Ligase and an optimized, ready-to-use 2X reaction buffer containing a proprietary ligation enhancer and no PEG. This combination is specifically formulated to promote robust ligation of all types of DNA ends (e.g., blunt, sticky, TA). It is directly compatible with electrocompetent cells used for transformation by electroporation, without desalting or purification.
- Directly compatible with electrocompetent cells
- No thawing needed as it maintains liquid form at -20°C
- Not sure which ligase to choose? Refer to our DNA and RNA Ligase Properties Chart
What are the best conditions for DNA ligation?
What molar ratios should I use for DNA Ligation?
How do I choose the best DNA Ligase?
Why do I need to add PEG to my DNA ligation?
Traditional Cloning Workflow
Overview of PCR Cloning
ElectroLigase™ combines T4 DNA ligase and an optimized, ready-to-use 2X reaction buffer containing a proprietary ligation enhancer and no PEG. This combination is specifically formulated to promote robust ligation of all types of DNA ends (blunt, sticky, TA). It is directly compatible, without desalting or purification, with electrocompetent cells used for transformation by electroporation. No thawing of the buffer is required as it maintains a liquid state during storage at -20°C*, thereby simplifying reaction set-up. By providing an optimized ratio of enzyme and buffer components, users are able to rapidly ligate all types of DNA ends applying a short incubation time at room temperature. Ligations for subcloning can be carried out in small volumes with low concentrations, allowing users to conserve precious DNA samples. These reactions can be pipetted directly, without purification or dilution, to transform many strains of electrocompetent E. coli**.
* Freezers vary in their actual internal temperatures. Our testing demonstrates that the enzyme and buffer remain liquid at -20°C.
**ElectroLigase is also compatible with chemically competent strains of E. coli. Performance is generally around 50% efficiency, when compared to the Blunt/TA Ligase Master Mix (NEB #M0367 ).
Product SourcePurified from an E. coli strain containing a recombinant gene encoding T4 DNA Ligase.
Reaction Volume Definition1X ElectroLigase Reaction Buffer with DNA substrates and 1 μl ElectroLigase in an 11 μl reaction volume incubated at 25°C for 30 minutes.
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