T4 PDG (T4 Endonuclease V)
- DNA damage studies
- Single-cell gel electrophoresis (Comet Assay)
- Isolated from a recombinant source
- Supplied with 10X Reaction Buffer
T4 PDG (pyrimidine dimer glycosylase) has both DNA glycosylase and APlyase activity. The 16 kd protein recognizes cis-syn-cyclobutane pyrimidine dimers caused by UV irradiation. The enzyme cleaves the glycosyl bond of the 5´ end of the pyrimidine dimer and the endonucleolytic activity cleaves the phosphodiester bond at the AP site.
Product SourcePurified from an E. coli strain carrying a plasmid encoding T4 denV gene.
The following reagents are supplied with this product:
Store at (°C) Concentration T4 PDG Reaction Buffer -20 10 X Purified BSA -20
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