T4 PDG (T4 Endonuclease V)

Reagents Supplied

The following reagents are supplied with this product:

NEB #	Component Name	Component #	Stored at (°C)	Amount	Concentration
M0308S     -20       T4 PDG (T4 Endonuclease V) M0308SVIAL -20 1 x 0.2 ml 10,000 units/ml   T4 PDG Reaction Buffer B0308SVIAL -20 1 x 1.5 ml 10 X   Recombinant Albumin, Molecular Biology Grade B9200SVIAL -20 1 x 0.6 ml 20 mg/ml

Application Features

• DNA damage studies
• Single cell gel electrophoresis (comet assay)

Materials Sold Separately

• Purified BSA

1. Warm buffer to room temperature, as it precipitates at 4°C.
2. The enzyme has 100% activity in NEBuffer 4 (NEB #B7004) and 75% activity in NEBuffers 1-3 (NEB #B7001, NEB #B7002, NEB #B7003).
3. For best results, incubation time should be 30 minutes or less.
4. After the reaction, addition of SDS to a final concentration of 0.5%, or 1µl phenol, in 6X Loading dye to the sample before running the gel will improve electrophoresis by dissociating the protein from the DNA.

1. Higgins, K.L. and Lloyd, R.S. (1987). Mutation Research. 183, 117-121.

1. Protocol for T4 PDG (M0308)

• DNA Damage and PreCR

• Activities of DNA Repair Enzymes and Structure-specific Endonucleases
• DNA Repair Enzymes on Damaged and Non-standard Bases
• Properties of DNA Repair Enzymes and Structure-specific Endonucleases

1. What is the activity of T4 PDG in the NEBuffers 1-4?
2. What is the molecular weight of T4 PDG?
3. Is T4 PDG a tagged protein?
4. Can the T4 PDG be used in the comet assay?
5. Does T4 PDG remove the damaged base?
6. What type of damaged DNA does T4 PDG recognize?

Quality Control tests are performed on each new lot of NEB product to meet the specifications designated for it. Specifications and individual lot data from the tests that are performed for this particular product can be found and downloaded on the Product Specification Sheet, Certificate of Analysis, data card or product manual. Further information regarding NEB product quality can be found here.

Specifications & Change Notifications

The Specification sheet is a document that includes the storage temperature, shelf life and the specifications designated for the product. The following file naming structure is used to name these document files: [Product Number]_[Size]_[Version]

• M0308S_L_v1

The Certificate of Analysis (COA) is a signed document that includes the storage temperature, expiration date and quality controls for an individual lot. The following file naming structure is used to name these document files: [Product Number]_[Size]_[Version]_[Lot Number]

• M0308S_v1_10023165
• M0308S_v1_10043224
• M0308S_v1_10056717
• M0308S_v1_10059869
• M0308S_v1_10070060
• M0308S_v1_10092158
• M0308S_v1_10096931
• M0308S_v1_10100123
• M0308S_v1_10141027
• M0308S_v1_10161494
• M0308S_v1_10186789
• M0308S_v1_10189747

The following is a list of Safety Data Sheet (SDS) that apply to this product to help you use it safely.

• T4 PDG (T4 Endonuclease V)
• T4 PDG Reaction Buffer
• Recombinant Albumin, Molecular Biology Grade

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This product is intended for research purposes only. This product is not intended to be used for therapeutic or diagnostic purposes in humans or animals.

New England Biolabs (NEB) is committed to practicing ethical science – we believe it is our job as researchers to ask the important questions that when answered will help preserve our quality of life and the world that we live in. However, this research should always be done in safe and ethical manner. Learn more.

Licenses

Nucleic acid-based aptamers for use with reverse transcriptases are licensed exclusively by New England Biolabs, Inc. from SomaLogic, Inc. New England Biolabs, Inc. gives the Buyer/User a non-exclusive license to use the WarmStart® RTx Reverse Transcriptase for Research Use Only (RUO). Commercial use of this product may require a license from New England Biolabs, Inc. For additional information or to inquire about commercial use, please contact busdev@neb.com.