T4 PDG (T4 Endonuclease V)
- T4 pyrimidine DNA glycosylase
- Bifunctional DNA glycosylase with DNA N-glycosylase and AP lyase activities
- The N-glycosylase activity releases cis-syn cyclobutane pyrimidine dimers, including T^T, T^C and C^C, generating an AP site. The AP lyase activity cleaves an AP site via β-elimination, creating a 1 nucleotide DNA gap with 3'-α, β-unsaturated aldehyde and 5'-phosphate termini.
- Also known as T4 Endonuclease V
T4 PDG (pyrimidine dimer glycosylase) has both DNA glycosylase and APlyase activity. The 16 kd protein recognizes cis-syn-cyclobutane pyrimidine dimers caused by UV irradiation. The enzyme cleaves the glycosyl bond of the 5´ end of the pyrimidine dimer and the endonucleolytic activity cleaves the phosphodiester bond at the AP site.
Product SourcePurified from an E. coli strain carrying a plasmid encoding T4 denV gene.
The following reagents are supplied with this product:
Store at (°C) Concentration T4 PDG Reaction Buffer 10X Purified BSA -20 10 mg/ml
- Protocols, Manuals & Usage
- Tools & Resources
- FAQs & Troubleshooting
- Citations & Technical Literature
- Quality, Safety & Legal
Ineligible item added to cart
Based on your Freezer Program type, you are trying to add a product to your cart that is either not allowed or not allowed with the existing contents of your cart. Please review and update your order accordingly If you have any questions, please contact Customer Service at [email protected] or 1-800-632-5227 x 8.