RNase Inhibitor, Human Placenta

Reagents Supplied

The following reagents are supplied with this product:

NEB #	Component Name	Component #	Stored at (°C)	Amount	Concentration
M0307S     -20       RNase Inhibitor, Human Placenta M0307SVIAL -20 1 x 0.05 ml 40,000 units/ml
M0307L     -20       RNase Inhibitor, Human Placenta M0307LVIAL -20 1 x 0.25 ml 40,000 units/ml

1. Since ribonucleases typically retain activity under denaturing conditions, care must be taken to avoid denaturing RNase Inhibitor molecules which have complexed with a ribonuclease. To prevent the release of active ribonuclease, temperatures greater than 50°C and high concentrations of urea or other denaturing agents should be avoided.
2. The recommended concentration of RNase Inhibitor in a reaction is 1 unit/μl. During assembly of a reaction, RNase Inhibitor should be added before other components that are possible sources of RNase contamination (i.e. enzymes, plasmid from a mini prep.)

1. Blackburn, P. and Moore, S. (1982). Pancreatic Ribonucleases . The Enzymes. XV, Part B, Academic Press, NY.
2. Blackburn, P., Wilson, G. and Moore, S. (1977). J. Biol. Chem. 252,5904.

1. Protocol for Avoiding Rnase Contamination using Rnase Inhibitor, Human Placenta (M0307)

Quality Control tests are performed on each new lot of NEB product to meet the specifications designated for it. Specifications and individual lot data from the tests that are performed for this particular product can be found and downloaded on the Product Specification Sheet, Certificate of Analysis, data card or product manual. Further information regarding NEB product quality can be found here.

Specifications & Change Notifications

The Specification sheet is a document that includes the storage temperature, shelf life and the specifications designated for the product. The following file naming structure is used to name these document files: [Product Number]_[Size]_[Version]

• M0307S_L_v1

The Certificate of Analysis (COA) is a signed document that includes the storage temperature, expiration date and quality controls for an individual lot. The following file naming structure is used to name these document files: [Product Number]_[Size]_[Version]_[Lot Number]

• M0307S_L_v1_0321505
• M0307S_L_v1_0321511
• M0307S_L_v1_0321610
• M0307S_L_v1_0331711
• M0307S_v1_10013343
• M0307L_v1_10013342
• M0307L_v1_10031693
• M0307S_v1_10031690
• M0307L_v1_10051892
• M0307L_v1_10054874
• M0307S_v1_10051895
• M0307S_v1_10059006
• M0307L_v1_10061044
• M0307L_v1_10070014
• M0307L_v1_10077778
• M0307S_v1_10070016
• M0307L_v1_10085509
• M0307L_v1_10106730
• M0307S_v1_10106707
• M0307L_v1_10106710
• M0307S_v1_10106729
• M0307L_v1_10115396
• M0307S_v1_10119670
• M0307L_v1_10139643
• M0307S_v1_10139640
• M0307L_v1_10171419
• M0307S_v1_10173154

The following is a list of Safety Data Sheet (SDS) that apply to this product to help you use it safely.

• RNase Inhibitor, Human Placenta

Products and content are covered by one or more patents, trademarks and/or copyrights owned or controlled by New England Biolabs, Inc (NEB). The use of trademark symbols does not necessarily indicate that the name is trademarked in the country where it is being read; it indicates where the content was originally developed. The use of this product may require the buyer to obtain additional third-party intellectual property rights for certain applications. For more information, please email busdev@neb.com.

This product is intended for research purposes only. This product is not intended to be used for therapeutic or diagnostic purposes in humans or animals.

New England Biolabs (NEB) is committed to practicing ethical science – we believe it is our job as researchers to ask the important questions that when answered will help preserve our quality of life and the world that we live in. However, this research should always be done in safe and ethical manner. Learn more.