Tma Endonuclease III
Tma Endonuclease III is a thermostable homolog of E. coli Endonuclease III, the product of the E. coli nth gene (1). It possesses both N-glycosylase and AP-lyase activities. The N-glycosylase activity releases damaged pyrimidines from double-stranded DNA, generating an abasic site. The AP-lysase activity cleaves the phosphodiester bond 3´ to the abasic site resulting in a 3´-α, β-unsaturated aldehyde and a 5´-phosphate.
Damaged bases recognized and removed by Tma Endonuclease III include 5,6 dihydroxythymine, thymine glycol and abasic sites (2).
Product SourceCloned from Thermotoga maritima and overexpressed in E. coli.
The following reagents are supplied with this product:
Store at (°C) Concentration ThermoPol® Reaction Buffer Pack -20 10 X
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