Tma Endonuclease III

Reagents Supplied

The following reagents are supplied with this product:

NEB #	Component Name	Component #	Stored at (°C)	Amount	Concentration
M0291S     -20       Tma Endonuclease III M0291SVIAL -20 1 x 0.05 ml 10,000 units/ml   ThermoPol® Reaction Buffer B9004SVIAL -20 1 x 1.5 ml 10 X

Application Features

• Alkaline elution (3)
• Alkaline unwinding (4)

Materials Sold Separately

• ThermoPol® Reaction Buffer Pack

1. Place reaction in Thermocycler or overlay with mineral oil to reduce evaporation. 
2. Tma Endo III has 75% activity in ThermoPol^® II (Mg-free) Reaction Buffer (NEB #B9005).
3. Tma Endo III has a 100% activity in NEBuffer 1 (NEB# B7001), 100% activity in NEBuffer 4 (NEB #B7004), and 50% activity in NEBuffer 2 (NEB #B7002). The use of Tma Endo III in NEBuffer 3 (NEB #B7003) is not recommended.
4. Diluent Compatibility:   Diluent D:
   100 mM KCl, 10 mM Tris-HCl (pH 7.4 @ 25°C), 0.1 mM EDTA, 1 mM DTT, 0.1% Triton X-100 and 50% glycerol.

1. Nelson, K.E. et al. (1999). Nature. 399,
2. Marks, K. unpublished observation.
3. Pflaum, M. et al. (1998). FreeRad. Res. 29,
4. Hartwig, A. et al. (1996). Toxicol. Lett. 88,

1. Protocol for Tma Endonuclease III (M0291)

• DNA Damage and PreCR

• Activities of DNA Repair Enzymes and Structure-specific Endonucleases
• DNA Repair Enzymes on Damaged and Non-standard Bases
• Properties of DNA Repair Enzymes and Structure-specific Endonucleases

1. What is the activity of Tma Endonuclease III in the NEBuffers 1-4?
2. What is the activity of Tma Endonuclease III in ThermoPol buffer?
3. What is the molecular weight of Tma Endonuclease III?
4. Is Tma Endonuclease III a tagged protein?
5. What substrate is used to test the Tma Endonuclease III activity?
6. What types of DNA damage does Tma Endonuclease III act upon?

Quality Control tests are performed on each new lot of NEB product to meet the specifications designated for it. Specifications and individual lot data from the tests that are performed for this particular product can be found and downloaded on the Product Specification Sheet, Certificate of Analysis, data card or product manual. Further information regarding NEB product quality can be found here.

Specifications & Change Notifications

The Specification sheet is a document that includes the storage temperature, shelf life and the specifications designated for the product. The following file naming structure is used to name these document files: [Product Number]_[Size]_[Version]

• M0291S_L_v1

The Certificate of Analysis (COA) is a signed document that includes the storage temperature, expiration date and quality controls for an individual lot. The following file naming structure is used to name these document files: [Product Number]_[Size]_[Version]_[Lot Number]

• M0291S_L_v1_0011707
• M0291S_L_v1_0011804
• M0291S_v1_10039442
• M0291S_v1_10054887
• M0291S_v1_10063336
• M0291S_v1_10069696
• M0291S_v1_10109991
• M0291S_v1_10154970

The following is a list of Safety Data Sheet (SDS) that apply to this product to help you use it safely.

• Tma Endonuclease III
• ThermoPol^® Reaction Buffer

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This product is intended for research purposes only. This product is not intended to be used for therapeutic or diagnostic purposes in humans or animals.

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