Application Features

• PCR
• Primer extension
• Colony PCR

Unit Definition

One unit is defined as the amount of enzyme that will incorporate 15 nmol of dNTP into acid-insoluble material in 30 minutes at 75°C.

Reaction Conditions

1X ThermoPol^® Reaction Buffer Pack

1X ThermoPol^® Reaction Buffer Pack
20 mM Tris-HCl
10 mM (NH₄)₂SO₄
10 mM KCl
2 mM MgSO₄
0.1% Triton® X-100
(pH 8.8 @ 25°C)

Storage Temperature

-20°C

Storage Conditions

10 mM Tris-HCl
100 mM KCl
1 mM DTT
0.1 mM EDTA
0.5% Tween® 20
0.5% IGEPAL® CA-630
50% Glycerol
pH 7.4 @ 25°C

Heat Inactivation

No

Molecular Weight

Theoretical: 94000 daltons

5' - 3' Exonuclease

Yes

3' - 5' Exonuclease

No

Strand Displacement

+

Resulting Ends

• Single-base 3´ Overhangs

Unit Assay Conditions

1X ThermoPol Reaction Buffer, 200 µM dNTPs including [³H]-dTTP and 15 nM primed M13 DNA. 

Error Rate

~ 285x10^-6bases

Companion Products

• Magnesium Sulfate (MgSO₄) Solution
• ThermoPol® II (Mg-free) Reaction Buffer Pack
• Taq PCR Kit
• Taq 2X Master Mix
• Quick-Load® Taq 2X Master Mix
• Taq 5X Master Mix
• Deoxynucleotide (dNTP) Solution Set
• Deoxynucleotide (dNTP) Solution Mix

Materials Sold Separately

• ThermoPol® Reaction Buffer Pack

1. 5'→3' flap endonuclease activity destroys displaced strand.

1. Chien, A., Edgar, D.B. and Trela, J.M. (1976). J. Bact.. 127, 1550-1557.
2. Kaledin, A.S., Sliusarenko, A.G. and Gorodetskii, S.I. (1980). Biokhimiya. 45, 644-651.
3. Lawyer, F.C. et al. (1993). PCR Methods and Appl.. 2, 275-287.
4. Longley, M.J., Bennett, S.E. and Mosbaugh D.W. (1990). Nucleic Acids Res.. 18, 7317-7322.
5. Lyamichev, V., Brow, M.A. and Dahlberg, J.E. (1993). Science. 260, 778-783.
6. Saiki R.K. et al. (1985). Science. 230, 1350-1354.
7. Powell, L.M. et al. (1987). Cell. 50, 831-840.
8. Sun, Y., Hegamyer, G. and Colburn, N. (1993). Biotechniques. 15, 372-374.
9. Sarkar, G., Kapelner, S. and Sommer, S.S. (1990). Nucleic Acids Res.. 18, 7465.

1. Can Taq DNA Polymerase be used in other buffers?
2. Why is the product a smear when visualized on an agarose gel?
3. Why is there no product when visualized on an agarose gel?
4. How should I set up an amplification reaction using Taq DNA Polymerase?
5. What type of DNA ends result from a primer extension reaction or a PCR using Taq DNA Polymerase?
6. The product sequence doesn't completely match the expected sequence. How can this result be improved?
7. When should Taq DNA Polymerase be used in a primer extension reaction or for PCR?
8. Will the 5'→3' flap endonuclease activity of Taq DNA Polymerase degrade primers?
9. Can Taq DNA Polymerase be used for nick translation?
10. Does the presence of Ca²⁺ inhibit PCR?

1. PCR Protocol for Taq DNA Polymerase with ThermoPol^® Buffer (M0267)
2. A-Tailing with Taq Polymerase

• Buffer and Diluent Formulation Table
• DNA Polymerase Selection Chart
• PCR Selection Chart
• Taq Buffer Selection Chart
• Thermophilic DNA Polymerases

• Activity of Restriction Enzymes in PCR Buffers
• Guidelines for PCR Optimization with Taq DNA Polymerase
• Guidelines for PCR Optimization with Thermophilic DNA Polymerases

• PCR Troubleshooting Guide
• Taq PCR Kit Troubleshooting Guide

• NEBioCalculator
• PCR Selector
• Tm Calculator

Quality Control tests are performed on each new lot of NEB product to meet the specifications designated for it. Specifications and individual lot data from the tests that are performed for this particular product can be found and downloaded on the Product Specification Sheet, Certificate of Analysis, data card or product manual. Further information regarding NEB product quality can be found here.

The Specification sheet is a document that includes the storage temperature, shelf life and the specifications designated for the product. The following file naming structure is used to name these document files: [Product Number]_[Size]_[Version]

• M0267S_L_X_E_v1

The Certificate of Analysis (COA) is a signed document that includes the storage temperature, expiration date and quality control's for an individual lot. The following file naming structure is used to name these document files: [Product Number]_[Size]_[Version]_[Lot Number]

• M0267S_L_X_E_v1_0141506
• M0267S_L_X_E_v1_0141606
• M0267S_L_X_E_v1_0141512
• M0267S_L_X_E_v1_0141612
• M0267S_L_X_E_v1_0141705
• M0267S_L_X_E_v1_0141706
• M0267S_L_X_E_v1_0141712

The following is a list of Safety Data Sheet (SDS) that apply to this product to help you use it safely.

• Taq DNA Polymerase with ThermoPol® Buffer
• ThermoPol^® Reaction Buffer Pack

This product is covered by one or more patents, trademarks and/or copyrights owned or controlled by New England Biolabs, Inc (NEB).

While NEB develops and validates its products for various applications, the use of this product may require the buyer to obtain additional third party intellectual property rights for certain applications.

For more information about commercial rights, please contact NEB's Global Business Development team at gbd@neb.com.

This product is intended for research purposes only. This product is not intended to be used for therapeutic or diagnostic purposes in humans or animals.

Trademarks

NEW ENGLAND BIOLABS®, QUICK-LOAD® and THERMOPOL® are registered trademarks of New England Biolabs, Inc.

IGEPAL^® is a registered trademark of Rhodia Operations.
TRITON^® is a registered trademark of Union Carbide Corporation
TWEEN^® is a registered trademark of Uniqema Americas, LLC.