Home PCR, Polymerases & Amplification Technologies Products Taq DNA Polymerase with ThermoPol® Buffer

Taq DNA Polymerase with ThermoPol® Buffer

Taq DNA Polymerase is the industry standard for PCR

Ideal for routine PCR applications
ThermoPol Buffer delivers high product yield under demanding conditions
Exceptional value in terms of cost per unit

Ordering Information

Catalog #

Concentration

5,000 units/ml

Size

400 units

List Price

$61.00

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Concentration

5,000 units/ml

Size

2,000 units

List Price

$244.00

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Quantity
Concentration

5,000 units/ml

Size

4,000 units

List Price

$443.00

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Quantity
Concentration

5,000 units/ml

Size

20,000 units

List Price

$1,200.00

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Quantity

Did you know this product can be customized or purchased in larger volumes? Submit an inquiry to find out more about customization options.

Product Information

Taq DNA Polymerase is a thermostable DNA polymerase that possesses a 5´→3´ polymerase activity (1,2,3) and a 5´ flap endonuclease activity (4,5).

It is supplied with 10X ThermoPol Reaction Buffer, which contains a nonionic detergent to increase enzyme stability during longer incubations.

Highlights

Isolated from a recombinant source
Supplied with 10X Reaction Buffer
Robust and reliable reactions
Tolerates a wide range of templates
Incorporates dUTP, dITP and fluorescently-labeled nucleotides
Exceptional value in terms of cost per unit

Product Source

An E. coli strain that carries the Taq DNA Polymerase gene from Thermus aquaticus YT-1

Reagents Supplied

The following reagents are supplied with this product:

	Store at (°C)	Concentration
ThermoPol^®Reaction Buffer Pack	-20	10 X

Product Categories:: Taq DNA Polymerase Products

Applications:: A-tailing,; Colony PCR,; Polymerases for DNA Manipulation; Multiplex PCR; Specialty PCR; Routine PCR; PCR

Advantages and Features
Application Features
- PCR
- Primer extension
- Colony PCR
Properties and Usage
Unit Definition
One unit is defined as the amount of enzyme that will incorporate 15 nmol of dNTP into acid-insoluble material in 30 minutes at 75°C.

Reaction Conditions

1X ThermoPol^® Reaction Buffer Pack

1X ThermoPol^® Reaction Buffer Pack
20 mM Tris-HCl
10 mM (NH₄)₂SO₄
10 mM KCl
2 mM MgSO₄
0.1% Triton® X-100
(pH 8.8 @ 25°C)

Storage Temperature
-20°C
Storage Conditions

10 mM Tris-HCl
100 mM KCl
1 mM DTT
0.1 mM EDTA
0.5% Tween® 20
0.5% IGEPAL® CA-630
50% Glycerol
pH 7.4 @ 25°C

Heat Inactivation
No
Molecular Weight
Theoretical: 94000 daltons
5' - 3' Exonuclease
Yes
3' - 5' Exonuclease
No
Strand Displacement
+
Resulting Ends
- Single-base 3´ Overhangs
Unit Assay Conditions
1X ThermoPol Reaction Buffer, 200 µM dNTPs including [³H]-dTTP and 15 nM primed M13 DNA.
Error Rate
~ 285x10^-6bases
Related Products
Companion Products
Materials Sold Separately
- ThermoPol® Reaction Buffer Pack
Notes
1. 5'→3' flap endonuclease activity destroys displaced strand.
References
1. Chien, A., Edgar, D.B. and Trela, J.M. (1976). J. Bact.. 127, 1550-1557.
2. Kaledin, A.S., Sliusarenko, A.G. and Gorodetskii, S.I. (1980). Biokhimiya. 45, 644-651.
3. Lawyer, F.C. et al. (1993). PCR Methods and Appl.. 2, 275-287.
4. Longley, M.J., Bennett, S.E. and Mosbaugh D.W. (1990). Nucleic Acids Res.. 18, 7317-7322.
5. Lyamichev, V., Brow, M.A. and Dahlberg, J.E. (1993). Science. 260, 778-783.
6. Saiki R.K. et al. (1985). Science. 230, 1350-1354.
7. Powell, L.M. et al. (1987). Cell. 50, 831-840.
8. Sun, Y., Hegamyer, G. and Colburn, N. (1993). Biotechniques. 15, 372-374.
9. Sarkar, G., Kapelner, S. and Sommer, S.S. (1990). Nucleic Acids Res.. 18, 7465.

FAQs & Tech Tips
- FAQs
  Can Taq DNA Polymerase be used in other buffers?
  
  Why is the product a smear when visualized on an agarose gel?
  
  Why is there no product when visualized on an agarose gel?
  
  How should I set up an amplification reaction using Taq DNA Polymerase?
  
  What type of DNA ends result from a primer extension reaction or a PCR using Taq DNA Polymerase?
  
  The product sequence doesn't completely match the expected sequence. How can this result be improved?
  
  When should Taq DNA Polymerase be used in a primer extension reaction or for PCR?
  
  Will the 5'→3' flap endonuclease activity of Taq DNA Polymerase degrade primers?
  
  Can Taq DNA Polymerase be used for nick translation?
  
  Does the presence of Ca2+ inhibit PCR?
- Tech Tips
  
  Did you know most Taq reactions amplify more efficiently and robustly when you use a 68°C extension temperature?
Protocols & Manuals
- Protocols
  PCR Protocol for Taq DNA Polymerase with ThermoPol Buffer (M0267)
  
  A-Tailing with Taq Polymerase
- Datacards
  The Product Summary Sheet, or Data Card, includes details for how to use the product, as well as details of its formulation and quality controls. The following file naming structure is used to name the majority of these document files: [Catalog Number]Datasheet-Lot[Lot Number]. For those product lots not listed below, please contact NEB at info@neb.com or fill out the Technical Support Form for appropriate document.)
  
  M0267Datasheet-Lot0141203
  
  M0267Datasheet-Lot0141206
  
  M0267Datasheet-Lot0141212
  
  M0267Datasheet-Lot0141306
  
  M0267Datasheet-Lot0141312
  
  M0267Datasheet-Lot0141406
  
  M0267Datasheet-Lot0141412
  
  M0267Datasheet-Lot0151506
Other Tools & Resources
Quality & Safety
- Quality Control Assays
  
  Quality Control tests are performed on each new lot of NEB product to meet the specifications designated for it. Specifications and individual lot data from the tests that are performed for this particular product can be found and downloaded on the Product Specification Sheet, Certificate of Analysis, data card or product manual. Further information regarding NEB product quality can be found here.
- Specifications
  The Specification sheet is a document that includes the storage temperature, shelf life and the specifications designated for the product. The following file naming structure is used to name these document files: [Product Number]_[Size]_[Version]
  
  M0267S_L_X_E_v1
- Certificate of Analysis
  The Certificate of Analysis (COA) is a signed document that includes the storage temperature, expiration date and quality controls for an individual lot. The following file naming structure is used to name these document files: [Product Number]_[Size]_[Version]_[Lot Number]
  
  M0267S_L_X_E_v1_0141506
  
  M0267S_L_X_E_v1_0141606
  
  M0267S_L_X_E_v1_0141512
  
  M0267S_L_X_E_v1_0141612
  
  M0267S_L_X_E_v1_0141705
  
  M0267S_L_X_E_v1_0141706
  
  M0267S_L_X_E_v1_0141712
  
  M0267L_v1_10007346
  
  M0267E_v1_10015579
  
  M0267X_v1_10015580
  
  M0267S_v1_10015721
  
  M0267X_v1_10019645
  
  M0267X_v1_10019973
  
  M0267E_v1_10021952
  
  M0267L_v1_10015837
- Safety Data Sheets
  The following is a list of Safety Data Sheet (SDS) that apply to this product to help you use it safely.
  
  Taq DNA Polymerase with ThermoPol® Buffer
  
  ThermoPol^®Reaction Buffer Pack
- Datacards
  The Product Summary Sheet, or Data Card, includes details for how to use the product, as well as details of its formulation and quality controls. The following file naming structure is used to name the majority of these document files: [Catalog Number]Datasheet-Lot[Lot Number]. For those product lots not listed below, please contact NEB at info@neb.com or fill out the Technical Support Form for appropriate document.)
  
  M0267Datasheet-Lot0141203
  
  M0267Datasheet-Lot0141206
  
  M0267Datasheet-Lot0141212
  
  M0267Datasheet-Lot0141306
  
  M0267Datasheet-Lot0141312
  
  M0267Datasheet-Lot0141406
  
  M0267Datasheet-Lot0141412
  
  M0267Datasheet-Lot0151506
Legal Information
- Legal And Disclaimers
  
  This product is covered by one or more patents, trademarks and/or copyrights owned or controlled by New England Biolabs, Inc (NEB).
  
  While NEB develops and validates its products for various applications, the use of this product may require the buyer to obtain additional third party intellectual property rights for certain applications.
  
  For more information about commercial rights, please contact NEB's Global Business Development team at gbd@neb.com.
  
  This product is intended for research purposes only. This product is not intended to be used for therapeutic or diagnostic purposes in humans or animals.
  Trademarks
  NEW ENGLAND BIOLABS®, QUICK-LOAD® and THERMOPOL® are registered trademarks of New England Biolabs, Inc.
  
  IGEPAL^® is a registered trademark of Rhodia Operations.
  TRITON^®is a registered trademark of Union Carbide Corporation
  TWEEN^® is a registered trademark of Uniqema Americas, LLC.

Taq DNA Polymerase with ThermoPol® Buffer

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Ordering Information

Highlights

Product Source

Reagents Supplied

Application Features

Unit Definition

Reaction Conditions

Storage Temperature

Storage Conditions

Heat Inactivation

Molecular Weight

5' - 3' Exonuclease

3' - 5' Exonuclease

Strand Displacement

Resulting Ends

Unit Assay Conditions

Error Rate

Companion Products

Materials Sold Separately

Trademarks

Also Recommended

Deoxynucleotide (dNTP) Solution Set

Deoxynucleotide (dNTP) Solution Mix

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