Home PCR, Polymerases & Amplification Technologies Products Taq DNA Polymerase with ThermoPol® Buffer

Taq DNA Polymerase with ThermoPol® Buffer

Taq DNA Polymerase is the industry standard for PCR

Ideal for routine PCR applications
ThermoPol Buffer delivers high product yield under demanding conditions
Exceptional value in terms of cost per unit

Ordering Information

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Product Information
Taq DNA Polymerase is a thermostable DNA polymerase that possesses a 5´→3´ polymerase activity (1,2,3) and a 5´ flap endonuclease activity (4,5).

It is supplied with 10X ThermoPol Reaction Buffer, which contains a nonionic detergent to increase enzyme stability during longer incubations.

Highlights
- Isolated from a recombinant source
- Supplied with 10X Reaction Buffer
- Robust and reliable reactions
- Tolerates a wide range of templates
- Incorporates dUTP, dITP and fluorescently-labeled nucleotides
- Exceptional value in terms of cost per unit
Product Source
An E. coli strain that carries the Taq DNA Polymerase gene from Thermus aquaticus YT-1
Reagents Supplied

The following reagents are supplied with this product:

Store at (°C) Concentration

ThermoPol^®Reaction Buffer Pack -20 10 X

Product Categories:

Taq DNA Polymerase Products

Applications:

A-tailing,

Colony PCR,

Polymerases for DNA Manipulation
,
Multiplex PCR
,
Specialty PCR
,
Routine PCR
,
PCR
- Advantages and Features
  Application Features
  
  PCR
  
  Primer extension
  
  Colony PCR
- Properties and Usage
  Unit Definition
  One unit is defined as the amount of enzyme that will incorporate 15 nmol of dNTP into acid-insoluble material in 30 minutes at 75°C.
  
  Reaction Conditions
  
  1X ThermoPol^® Reaction Buffer Pack
  
  1X ThermoPol^® Reaction Buffer Pack
  20 mM Tris-HCl
  10 mM (NH₄)₂SO₄
  10 mM KCl
  2 mM MgSO₄
  0.1% Triton® X-100
  (pH 8.8 @ 25°C)
  
  Storage Temperature
  -20°C
  Storage Conditions
  
  10 mM Tris-HCl
  100 mM KCl
  1 mM DTT
  0.1 mM EDTA
  0.5% Tween® 20
  0.5% IGEPAL® CA-630
  50% Glycerol
  pH 7.4 @ 25°C
  
  Heat Inactivation
  No
  Molecular Weight
  Theoretical: 94000 daltons
  5' - 3' Exonuclease
  Yes
  3' - 5' Exonuclease
  No
  Strand Displacement
  +
  Resulting Ends
  
  Single-base 3´ Overhangs
  
  Unit Assay Conditions
  1X ThermoPol Reaction Buffer, 200 µM dNTPs including [³H]-dTTP and 15 nM primed M13 DNA.
  Error Rate
  ~ 285x10^-6bases
- Related Products
  Companion Products
  
  Magnesium Sulfate (MgSO₄) Solution
  
  ThermoPol® II (Mg-free) Reaction Buffer Pack
  
  Taq PCR Kit
  
  Taq 2X Master Mix
  
  Quick-Load® Taq 2X Master Mix
  
  Taq 5X Master Mix
  
  Deoxynucleotide (dNTP) Solution Set
  
  Deoxynucleotide (dNTP) Solution Mix
  
  Materials Sold Separately
  
  ThermoPol® Reaction Buffer Pack
- Notes
  5'→3' flap endonuclease activity destroys displaced strand.
- References
  Chien, A., Edgar, D.B. and Trela, J.M. (1976). J. Bact.. 127, 1550-1557.
  
  Kaledin, A.S., Sliusarenko, A.G. and Gorodetskii, S.I. (1980). Biokhimiya. 45, 644-651.
  
  Lawyer, F.C. et al. (1993). PCR Methods and Appl.. 2, 275-287.
  
  Longley, M.J., Bennett, S.E. and Mosbaugh D.W. (1990). Nucleic Acids Res.. 18, 7317-7322.
  
  Lyamichev, V., Brow, M.A. and Dahlberg, J.E. (1993). Science. 260, 778-783.
  
  Saiki R.K. et al. (1985). Science. 230, 1350-1354.
  
  Powell, L.M. et al. (1987). Cell. 50, 831-840.
  
  Sun, Y., Hegamyer, G. and Colburn, N. (1993). Biotechniques. 15, 372-374.
  
  Sarkar, G., Kapelner, S. and Sommer, S.S. (1990). Nucleic Acids Res.. 18, 7465.
FAQs & Tech Tips
- FAQs
  Can Taq DNA Polymerase be used in other buffers?
  
  Why is the product a smear when visualized on an agarose gel?
  
  Why is there no product when visualized on an agarose gel?
  
  How should I set up an amplification reaction using Taq DNA Polymerase?
  
  What type of DNA ends result from a primer extension reaction or a PCR using Taq DNA Polymerase?
  
  The product sequence doesn't completely match the expected sequence. How can this result be improved?
  
  When should Taq DNA Polymerase be used in a primer extension reaction or for PCR?
  
  Will the 5'→3' flap endonuclease activity of Taq DNA Polymerase degrade primers?
  
  Can Taq DNA Polymerase be used for nick translation?
  
  Does the presence of Ca2+ inhibit PCR?
- Tech Tips
  
  Did you know most Taq reactions amplify more efficiently and robustly when you use a 68°C extension temperature?
Protocols & Manuals
- Protocols
  PCR Protocol for Taq DNA Polymerase with ThermoPol Buffer (M0267)
  
  A-Tailing with Taq Polymerase
- Datacards
  The Product Summary Sheet, or Data Card, includes details for how to use the product, as well as details of its formulation and quality controls. The following file naming structure is used to name the majority of these document files: [Catalog Number]Datasheet-Lot[Lot Number]. For those product lots not listed below, please contact NEB at [email protected] or fill out the Technical Support Form for appropriate document.)
  
  M0267Datasheet-Lot0141203
  
  M0267Datasheet-Lot0141206
  
  M0267Datasheet-Lot0141212
  
  M0267Datasheet-Lot0141306
  
  M0267Datasheet-Lot0141312
  
  M0267Datasheet-Lot0141406
  
  M0267Datasheet-Lot0141412
  
  M0267Datasheet-Lot0151506
Other Tools & Resources
Quality & Safety
- Quality Control Assays
  
  Quality Control tests are performed on each new lot of NEB product to meet the specifications designated for it. Specifications and individual lot data from the tests that are performed for this particular product can be found and downloaded on the Product Specification Sheet, Certificate of Analysis, data card or product manual. Further information regarding NEB product quality can be found here.
- Specifications
  The Specification sheet is a document that includes the storage temperature, shelf life and the specifications designated for the product. The following file naming structure is used to name these document files: [Product Number]_[Size]_[Version]
  
  M0267S_L_X_E_v1
- Certificate of Analysis
  The Certificate of Analysis (COA) is a signed document that includes the storage temperature, expiration date and quality controls for an individual lot. The following file naming structure is used to name these document files: [Product Number]_[Size]_[Version]_[Lot Number]
  
  M0267S_L_X_E_v1_0141506
  
  M0267S_L_X_E_v1_0141606
  
  M0267S_L_X_E_v1_0141512
  
  M0267S_L_X_E_v1_0141612
  
  M0267S_L_X_E_v1_0141705
  
  M0267S_L_X_E_v1_0141706
  
  M0267S_L_X_E_v1_0141712
  
  M0267L_v1_10007346
  
  M0267E_v1_10015579
  
  M0267X_v1_10015580
  
  M0267S_v1_10015721
  
  M0267X_v1_10019645
  
  M0267X_v1_10019973
  
  M0267E_v1_10021952
  
  M0267L_v1_10015837
  
  M0267S_v1_10017230
- Safety Data Sheets
  The following is a list of Safety Data Sheet (SDS) that apply to this product to help you use it safely.
  
  Taq DNA Polymerase with ThermoPol® Buffer
  
  ThermoPol^®Reaction Buffer Pack
- Datacards
  The Product Summary Sheet, or Data Card, includes details for how to use the product, as well as details of its formulation and quality controls. The following file naming structure is used to name the majority of these document files: [Catalog Number]Datasheet-Lot[Lot Number]. For those product lots not listed below, please contact NEB at [email protected] or fill out the Technical Support Form for appropriate document.)
  
  M0267Datasheet-Lot0141203
  
  M0267Datasheet-Lot0141206
  
  M0267Datasheet-Lot0141212
  
  M0267Datasheet-Lot0141306
  
  M0267Datasheet-Lot0141312
  
  M0267Datasheet-Lot0141406
  
  M0267Datasheet-Lot0141412
  
  M0267Datasheet-Lot0151506
Legal Information
- Legal And Disclaimers
  
  This product is covered by one or more patents, trademarks and/or copyrights owned or controlled by New England Biolabs, Inc (NEB).
  
  While NEB develops and validates its products for various applications, the use of this product may require the buyer to obtain additional third party intellectual property rights for certain applications.
  
  For more information about commercial rights, please contact NEB's Global Business Development team at [email protected].
  
  This product is intended for research purposes only. This product is not intended to be used for therapeutic or diagnostic purposes in humans or animals.
  Trademarks
  NEW ENGLAND BIOLABS®, QUICK-LOAD® and THERMOPOL® are registered trademarks of New England Biolabs, Inc.
  
  IGEPAL^® is a registered trademark of Rhodia Operations.
  TRITON^®is a registered trademark of Union Carbide Corporation
  TWEEN^® is a registered trademark of Uniqema Americas, LLC.

	Store at (°C)	Concentration
ThermoPol^®Reaction Buffer Pack	-20	10 X

Taq DNA Polymerase with ThermoPol® Buffer

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Ordering Information

Highlights

Product Source

Reagents Supplied

Application Features

Unit Definition

Reaction Conditions

Storage Temperature

Storage Conditions

Heat Inactivation

Molecular Weight

5' - 3' Exonuclease

3' - 5' Exonuclease

Strand Displacement

Resulting Ends

Unit Assay Conditions

Error Rate

Companion Products

Materials Sold Separately

Trademarks

Also Recommended

Deoxynucleotide (dNTP) Solution Mix

Deoxynucleotide (dNTP) Solution Set

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