Taq DNA Polymerase with ThermoPol^® Buffer

Reagents Supplied

The following reagents are supplied with this product:

NEB #	Component Name	Component #	Stored at (°C)	Amount	Concentration
M0267S     -20       Taq DNA Polymerase with ThermoPol® Buffer M0267SVIAL -20 1 x 0.08 ml 5,000 units/ml   ThermoPol® Reaction Buffer Pack B9004SVIAL -20 2 x 1.5 ml 10 X
M0267L     -20       Taq DNA Polymerase with ThermoPol® Buffer M0267LVIAL -20 1 x 0.4 ml 5,000 units/ml   ThermoPol® Reaction Buffer Pack B9004SVIAL -20 4 x 1.5 ml 10 X
M0267X     -20       Taq DNA Polymerase with ThermoPol® Buffer M0267XVIAL -20 1 x 0.8 ml 5,000 units/ml   ThermoPol® Reaction Buffer Pack B9004SVIAL -20 8 x 1.5 ml 10 X
M0267E     -20       Taq DNA Polymerase with ThermoPol® Buffer M0267XVIAL -20 5 x 0.8 ml 5,000 units/ml   ThermoPol® Reaction Buffer Pack B9004SVIAL -20 40 x 1.5 ml 10 X

Application Features

• PCR
• Primer extension
• Colony PCR

Companion Products

• Taq 5X Master Mix
• Taq 2X Master Mix
• Taq PCR Kit
• Deoxynucleotide (dNTP) Solution Mix
• Deoxynucleotide (dNTP) Solution Set
• Quick-Load® Taq 2X Master Mix
• Magnesium Sulfate (MgSO₄) Solution

Materials Sold Separately

• ThermoPol® Reaction Buffer Pack

1. 5'→3' flap endonuclease activity destroys displaced strand.

1. Chien, A., Edgar, D.B. and Trela, J.M. (1976). J. Bact.. 127, 1550-1557.
2. Kaledin, A.S., Sliusarenko, A.G. and Gorodetskii, S.I. (1980). Biokhimiya. 45, 644-651.
3. Lawyer, F.C. et al. (1993). PCRMethods and Appl.. 2, 275-287.
4. Longley, M.J., Bennett, S.E. and Mosbaugh D.W. (1990). NucleicAcids Res.. 18, 7317-7322.
5. Lyamichev, V., Brow, M.A. and Dahlberg, J.E. (1993). Science. 260, 778-783.
6. Saiki R.K. et al. (1985). Science. 230, 1350-1354.
7. Powell, L.M. et al. (1987). Cell. 50, 831-840.
8. Sun, Y., Hegamyer, G. and Colburn, N. (1993). Biotechniques. 15, 372-374.
9. Sarkar, G., Kapelner, S. and Sommer, S.S. (1990). Nucleic Acids Res.. 18, 7465.

1. PCR Protocol for Taq DNA Polymerase with ThermoPol^® Buffer (M0267)
2. A-Tailing with Taq Polymerase

• Activity of Restriction Enzymes in PCR Buffers
• Guidelines for PCR Optimization with Taq DNA Polymerase
• Guidelines for PCR Optimization with Thermophilic DNA Polymerases

• DNA Polymerase Selection Chart
• NEB Diluent and Buffer Table
• Taq Buffer Selection Chart
• Thermophilic DNA Polymerases

• PCR Selector
• Tm Calculator

1. Can Taq DNA Polymerase be used in other buffers?
2. Why is the product a smear when visualized on an agarose gel?
3. Why is there no product when visualized on an agarose gel?
4. How should I set up an amplification reaction using Taq DNA Polymerase?
5. What type of DNA ends result from a primer extension reaction or a PCR using Taq DNA Polymerase?
6. The product sequence doesn't completely match the expected sequence. How can this result be improved?
7. When should Taq DNA Polymerase be used in a primer extension reaction or for PCR?
8. Will the 5'→3' flap endonuclease activity of Taq DNA Polymerase degrade primers?
9. Can Taq DNA Polymerase be used for nick translation?
10. Does the presence of Ca²⁺ inhibit PCR?

• PCR Troubleshooting Guide
• Taq PCR Kit Troubleshooting Guide

Quality Control tests are performed on each new lot of NEB product to meet the specifications designated for it. Specifications and individual lot data from the tests that are performed for this particular product can be found and downloaded on the Product Specification Sheet, Certificate of Analysis, data card or product manual. Further information regarding NEB product quality can be found here.

Specifications & Change Notifications

The Specification sheet is a document that includes the storage temperature, shelf life and the specifications designated for the product. The following file naming structure is used to name these document files: [Product Number]_[Size]_[Version]

• M0267S_L_X_E_v1
• M0267S_L_X_E_v2

The Certificate of Analysis (COA) is a signed document that includes the storage temperature, expiration date and quality controls for an individual lot. The following file naming structure is used to name these document files: [Product Number]_[Size]_[Version]_[Lot Number]

• M0267S_L_X_E_v1_0141506
• M0267S_L_X_E_v1_0141606
• M0267S_L_X_E_v1_0141512
• M0267S_L_X_E_v1_0141612
• M0267S_L_X_E_v1_0141705
• M0267S_L_X_E_v1_0141706
• M0267S_L_X_E_v1_0141712
• M0267L_v1_10007346
• M0267E_v1_10015579
• M0267X_v1_10015580
• M0267S_v1_10015721
• M0267X_v1_10019645
• M0267X_v1_10019973
• M0267E_v1_10021952
• M0267L_v1_10015837
• M0267S_v1_10017230
• M0267E_v1_10030654
• M0267E_v1_10032700
• M0267S_v1_10029614
• M0267X_v1_10021953
• M0267S_v1_10033751
• M0267E_v1_10038750
• M0267L_v1_10033874
• M0267X_v1_10033859
• M0267S_v1_10041711
• M0267E_v1_10042534
• M0267X_v1_10042535
• M0267E_v1_10046984
• M0267L_v1_10042542
• M0267X_v1_10046983
• M0267S_v1_10046021
• M0267E_v1_10049322
• M0267L_v1_10049326
• M0267X_v1_10049713
• M0267E_v1_10055389
• M0267L_v1_10055388
• M0267S_v1_10055386
• M0267X_v1_10055387
• M0267X_v1_10055390
• M0267E_v1_10055895
• M0267X_v1_10055858
• M0267E_v1_10060585
• M0267L_v1_10060582
• M0267X_v1_10060583
• M0267E_v1_10062510
• M0267S_v1_10060586
• M0267X_v1_10062512
• M0267E_v1_10063686
• M0267X_v1_10065824
• M0267E_v2_10065822
• M0267S_v2_10065804
• M0267E_v2_10069930
• M0267L_v2_10065812
• M0267L_v2_10069931
• M0267X_v2_10068685
• M0267E_v2_10071643
• M0267S_v2_10069391
• M0267X_v2_10071642
• M0267E_v2_10073007
• M0267X_v2_10073008
• M0267S_v2_10076985
• M0267L_v2_10076984
• M0267X_v2_10079485
• M0267E_v2_10088155
• M0267X_v2_10089131
• M0267E_v2_10091669
• M0267S_v2_10087105
• M0267L_v2_10089003
• M0267E_v2_10093275
• M0267X_v2_10093274
• M0267S_v2_10093272
• M0267X_v2_10096262
• M0267E_v2_10096267
• M0267L_v2_10096264
• M0267X_v2_10100670
• M0267E_v2_10103380
• M0267S_v2_10103379
• M0267E_v2_10106869
• M0267L_v2_10108515
• M0267L_v2_10115515
• M0267S_v2_10108242
• M0267S_v2_10118262
• M0267X_v2_10106868
• M0267X_v2_10112841
• M0267E_v2_10119759
• M0267S_v2_10121781
• M0267X_v2_10114837
• M0267E_v2_10123931
• M0267L_v2_10123952
• M0267X_v2_10123930
• M0267E_v2_10127972
• M0267E_v2_10129160
• M0267L_v2_10129018
• M0267X_v2_10129159
• M0267E_v2_10132782
• M0267S_v2_10127753
• M0267X_v2_10131905
• M0267E_v2_10137195
• M0267X_v2_10137194
• M0267S_v2_10146110
• M0267S_v2_10152169
• M0267S_v2_10160885
• M0267E_v2_10142678
• M0267E_v2_10145566
• M0267E_v2_10148893
• M0267E_v2_10159334
• M0267E_v2_10161505
• M0267L_v2_10147869
• M0267L_v2_10162099
• M0267X_v2_10142680
• M0267X_v2_10145437
• M0267X_v2_10148892
• M0267X_v2_10154355
• M0267X_v2_10159335
• M0267E_v2_10165004
• M0267L_v2_10165005
• M0267X_v2_10163739
• M0267X_v2_10165513
• M0267S_v2_10165006
• M0267E_v2_10170101
• M0267X_v2_10170215
• M0267S_v2_10173611
• M0267L_v2_10173609
• M0267E_v2_10173604
• M0267X_v2_10173605
• M0267E_v2_10176708
• M0267X_v2_10176710

The following is a list of Safety Data Sheet (SDS) that apply to this product to help you use it safely.

• Taq DNA Polymerase with ThermoPol^® Buffer
• ThermoPol^® Reaction Buffer Pack

Products and content are covered by one or more patents, trademarks and/or copyrights owned or controlled by New England Biolabs, Inc (NEB). The use of trademark symbols does not necessarily indicate that the name is trademarked in the country where it is being read; it indicates where the content was originally developed. The use of this product may require the buyer to obtain additional third-party intellectual property rights for certain applications. For more information, please email busdev@neb.com.

This product is intended for research purposes only. This product is not intended to be used for therapeutic or diagnostic purposes in humans or animals.

New England Biolabs (NEB) is committed to practicing ethical science – we believe it is our job as researchers to ask the important questions that when answered will help preserve our quality of life and the world that we live in. However, this research should always be done in safe and ethical manner. Learn more.

Trademarks

NEW ENGLAND BIOLABS®, QUICK-LOAD® and THERMOPOL® are registered trademarks of New England Biolabs, Inc.

IGEPAL^® is a registered trademark of Rhodia Operations.
TRITON^® is a registered trademark of Union Carbide Corporation
TWEEN^® is a registered trademark of Uniqema Americas, LLC.