Taq DNA Polymerase with ThermoPol® Buffer

Catalog #	Concentration	Size	List Price	Your Price
M0267S	5,000 units/ml	400 units	$61.00	Sign In Sign In
M0267L	5,000 units/ml	2,000 units	$244.00	Sign In Sign In
M0267X	5,000 units/ml	4,000 units	$443.00	Sign In Sign In
M0267E	5,000 units/ml	20,000 units	$1,200.00	Sign In Sign In

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Product Information
Taq DNA Polymerase is a thermostable DNA polymerase that possesses a 5´→3´ polymerase activity (1,2,3) and a 5´ flap endonuclease activity (4,5).

It is supplied with 10X ThermoPol Reaction Buffer, which contains a nonionic detergent to increase enzyme stability during longer incubations.

Highlights
- Isolated from a recombinant source
- Supplied with 10X Reaction Buffer
- Robust and reliable reactions
- Tolerates a wide range of templates
- Incorporates dUTP, dITP and fluorescently-labeled nucleotides
- Exceptional value in terms of cost per unit
Product Source
An E. coli strain that carries the Taq DNA Polymerase gene from Thermus aquaticus YT-1
Reagents Supplied

The following reagents are supplied with this product:

Store at (°C) Concentration

ThermoPol^® Reaction Buffer Pack -20 10 X

Product Categories:

Taq DNA Polymerase Products

Applications:

Colony PCR,

A-tailing,

Polymerases for DNA Manipulation
,
Multiplex PCR
,
Specialty PCR
,
Routine PCR
,
PCR
- Advantages and Features
  Application Features
  
  PCR
  
  Primer extension
  
  Colony PCR
- Properties & Usage
  Unit Definition
  One unit is defined as the amount of enzyme that will incorporate 15 nmol of dNTP into acid-insoluble material in 30 minutes at 75°C.
  
  Reaction Conditions
  
  1X ThermoPol^® Reaction Buffer Pack
  
  1X ThermoPol^® Reaction Buffer Pack
  20 mM Tris-HCl
  10 mM (NH₄)₂SO₄
  10 mM KCl
  2 mM MgSO₄
  0.1% Triton® X-100
  (pH 8.8 @ 25°C)
  
  Storage Temperature
  -20°C
  Storage Conditions
  
  10 mM Tris-HCl
  100 mM KCl
  1 mM DTT
  0.1 mM EDTA
  0.5% Tween® 20
  0.5% IGEPAL® CA-630
  50% Glycerol
  pH 7.4 @ 25°C
  
  Heat Inactivation
  No
  Molecular Weight
  Theoretical: 94000 daltons
  5' - 3' Exonuclease
  Yes
  3' - 5' Exonuclease
  No
  Strand Displacement
  +
  Resulting Ends
  
  Single-base 3´ Overhangs
  
  Unit Assay Conditions
  1X ThermoPol Reaction Buffer, 200 µM dNTPs including [³H]-dTTP and 15 nM primed M13 DNA.
  Error Rate
  ~ 285x10^-6bases
- Related Products
  Companion Products
  
  Magnesium Sulfate (MgSO₄) Solution
  
  Taq PCR Kit
  
  Taq 2X Master Mix
  
  Quick-Load® Taq 2X Master Mix
  
  Taq 5X Master Mix
  
  Deoxynucleotide (dNTP) Solution Set
  
  Deoxynucleotide (dNTP) Solution Mix
  
  Materials Sold Separately
  
  ThermoPol® Reaction Buffer Pack
- Product Notes
  5'→3' flap endonuclease activity destroys displaced strand.
- References
  Chien, A., Edgar, D.B. and Trela, J.M. (1976). J. Bact.. 127, 1550-1557.
  
  Kaledin, A.S., Sliusarenko, A.G. and Gorodetskii, S.I. (1980). Biokhimiya. 45, 644-651.
  
  Lawyer, F.C. et al. (1993). PCRMethods and Appl.. 2, 275-287.
  
  Longley, M.J., Bennett, S.E. and Mosbaugh D.W. (1990). NucleicAcids Res.. 18, 7317-7322.
  
  Lyamichev, V., Brow, M.A. and Dahlberg, J.E. (1993). Science. 260, 778-783.
  
  Saiki R.K. et al. (1985). Science. 230, 1350-1354.
  
  Powell, L.M. et al. (1987). Cell. 50, 831-840.
  
  Sun, Y., Hegamyer, G. and Colburn, N. (1993). Biotechniques. 15, 372-374.
  
  Sarkar, G., Kapelner, S. and Sommer, S.S. (1990). Nucleic Acids Res.. 18, 7465.
Protocols, Manuals & Usage
- Protocols
  PCR Protocol for Taq DNA Polymerase with ThermoPol Buffer (M0267)
  
  A-Tailing with Taq Polymerase
- Usage Guidelines
  Activity of Restriction Enzymes in PCR Buffers
  
  Guidelines for PCR Optimization with Taq DNA Polymerase
  
  Guidelines for PCR Optimization with Thermophilic DNA Polymerases
- Datacards
  The Product Summary Sheet, or Data Card, includes details for how to use the product, as well as details of its formulation and quality controls. The following file naming structure is used to name the majority of these document files: [Catalog Number]Datasheet-Lot[Lot Number]. For those product lots not listed below, please contact NEB at [email protected] or fill out the Technical Support Form for appropriate document.)
  
  M0267Datasheet-Lot0141203
  
  M0267Datasheet-Lot0141206
  
  M0267Datasheet-Lot0141212
  
  M0267Datasheet-Lot0141306
  
  M0267Datasheet-Lot0141312
  
  M0267Datasheet-Lot0141406
  
  M0267Datasheet-Lot0141412
  
  M0267Datasheet-Lot0151506
Tools & Resources
- Selection Charts
  Buffer and Diluent Formulation Table
  
  DNA Polymerase Selection Chart
  
  PCR Selection Chart
  
  Taq Buffer Selection Chart
  
  Thermophilic DNA Polymerases
- Web Tools
  PCR Selector
  
  Tm Calculator
FAQs & Troubleshooting
- FAQs
  Can Taq DNA Polymerase be used in other buffers?
  
  Why is the product a smear when visualized on an agarose gel?
  
  Why is there no product when visualized on an agarose gel?
  
  How should I set up an amplification reaction using Taq DNA Polymerase?
  
  What type of DNA ends result from a primer extension reaction or a PCR using Taq DNA Polymerase?
  
  The product sequence doesn't completely match the expected sequence. How can this result be improved?
  
  When should Taq DNA Polymerase be used in a primer extension reaction or for PCR?
  
  Will the 5'→3' flap endonuclease activity of Taq DNA Polymerase degrade primers?
  
  Can Taq DNA Polymerase be used for nick translation?
  
  Does the presence of Ca2+ inhibit PCR?
- Troubleshooting
  PCR Troubleshooting Guide
  
  Taq PCR Kit Troubleshooting Guide
- Tech Tips
  
  Did you know most Taq reactions amplify more efficiently and robustly when you use a 68°C extension temperature?
Citations & Technical Literature
- Citations
  
  Product Citation Tool
  
  Powered by Bioz See more details on Bioz
Quality, Safety & Legal
- Quality Control Assays
  
  Quality Control tests are performed on each new lot of NEB product to meet the specifications designated for it. Specifications and individual lot data from the tests that are performed for this particular product can be found and downloaded on the Product Specification Sheet, Certificate of Analysis, data card or product manual. Further information regarding NEB product quality can be found here.
- Specifications
  The Specification sheet is a document that includes the storage temperature, shelf life and the specifications designated for the product. The following file naming structure is used to name these document files: [Product Number]_[Size]_[Version]
  
  M0267S_L_X_E_v1
- Certificate of Analysis
  The Certificate of Analysis (COA) is a signed document that includes the storage temperature, expiration date and quality controls for an individual lot. The following file naming structure is used to name these document files: [Product Number]_[Size]_[Version]_[Lot Number]
  
  M0267S_L_X_E_v1_0141506
  
  M0267S_L_X_E_v1_0141606
  
  M0267S_L_X_E_v1_0141512
  
  M0267S_L_X_E_v1_0141612
  
  M0267S_L_X_E_v1_0141705
  
  M0267S_L_X_E_v1_0141706
  
  M0267S_L_X_E_v1_0141712
  
  M0267L_v1_10007346
  
  M0267E_v1_10015579
  
  M0267X_v1_10015580
  
  M0267S_v1_10015721
  
  M0267X_v1_10019645
  
  M0267X_v1_10019973
  
  M0267E_v1_10021952
  
  M0267L_v1_10015837
  
  M0267S_v1_10017230
  
  M0267E_v1_10030654
  
  M0267E_v1_10032700
  
  M0267S_v1_10029614
  
  M0267X_v1_10021953
  
  M0267S_v1_10033751
  
  M0267E_v1_10038750
  
  M0267L_v1_10033874
  
  M0267X_v1_10033859
  
  M0267S_v1_10041711
  
  M0267E_v1_10042534
  
  M0267X_v1_10042535
  
  M0267E_v1_10046984
  
  M0267L_v1_10042542
  
  M0267X_v1_10046983
  
  M0267S_v1_10046021
  
  M0267E_v1_10049322
  
  M0267L_v1_10049326
  
  M0267X_v1_10049713
- Safety Data Sheets
  The following is a list of Safety Data Sheet (SDS) that apply to this product to help you use it safely.
  
  Taq DNA Polymerase with ThermoPol® Buffer
  
  ThermoPol^® Reaction Buffer Pack
- Datacards
  The Product Summary Sheet, or Data Card, includes details for how to use the product, as well as details of its formulation and quality controls. The following file naming structure is used to name the majority of these document files: [Catalog Number]Datasheet-Lot[Lot Number]. For those product lots not listed below, please contact NEB at [email protected] or fill out the Technical Support Form for appropriate document.)
  
  M0267Datasheet-Lot0141203
  
  M0267Datasheet-Lot0141206
  
  M0267Datasheet-Lot0141212
  
  M0267Datasheet-Lot0141306
  
  M0267Datasheet-Lot0141312
  
  M0267Datasheet-Lot0141406
  
  M0267Datasheet-Lot0141412
  
  M0267Datasheet-Lot0151506
- Legal and Disclaimers
  
  This product is covered by one or more patents, trademarks and/or copyrights owned or controlled by New England Biolabs, Inc (NEB).
  
  While NEB develops and validates its products for various applications, the use of this product may require the buyer to obtain additional third party intellectual property rights for certain applications.
  
  For more information about commercial rights, please contact NEB's Global Business Development team at [email protected].
  
  This product is intended for research purposes only. This product is not intended to be used for therapeutic or diagnostic purposes in humans or animals.
  Trademarks
  NEW ENGLAND BIOLABS®, QUICK-LOAD® and THERMOPOL® are registered trademarks of New England Biolabs, Inc.
  
  IGEPAL^® is a registered trademark of Rhodia Operations.
  TRITON^®is a registered trademark of Union Carbide Corporation
  TWEEN^® is a registered trademark of Uniqema Americas, LLC.

Applications

Product Categories

You’ll be Thrilled to Pieces

Tools

Resources

Applications

Product Categories

You’ll be Thrilled to Pieces

Tools

Resources

Taq DNA Polymerase with ThermoPol® Buffer

Highlights

Product Source

Reagents Supplied

Application Features

Unit Definition

Reaction Conditions

Storage Temperature

Storage Conditions

Heat Inactivation

Molecular Weight

5' - 3' Exonuclease

3' - 5' Exonuclease

Strand Displacement

Resulting Ends

Unit Assay Conditions

Error Rate

Companion Products

Materials Sold Separately

Product Citation Tool

Trademarks

Other Products You May Be Interested In

Deoxynucleotide (dNTP) Solution Mix

Deoxynucleotide (dNTP) Solution Set

	Store at (°C)	Concentration
ThermoPol^® Reaction Buffer Pack	-20	10 X

Taq DNA Polymerase with ThermoPol® Buffer

Featured Video

Choose the Right DNA Polymerase for PCR

Highlights

Product Source

Reagents Supplied

Application Features

Unit Definition

Reaction Conditions

Storage Temperature

Storage Conditions

Heat Inactivation

Molecular Weight

5' - 3' Exonuclease

3' - 5' Exonuclease

Strand Displacement

Resulting Ends

Unit Assay Conditions

Error Rate

Companion Products

Materials Sold Separately

Product Citation Tool

Trademarks

Featured Videos

Choose the Right DNA Polymerase for PCR

5 Tips for Setting Up Your PCR

Why is Tm Important in Primer Design?

Overview of PCR

Other Products You May Be Interested In

Deoxynucleotide (dNTP) Solution Mix

Deoxynucleotide (dNTP) Solution Set

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