M-MuLV Reverse Transcriptase
M-MuLV Reverse Transcriptase synthesizes a complementary DNA strand initiating from a primer using either RNA (cDNA synthesis) or single-stranded DNA as a template.
- Isolated from a recombinant source
- Supplied with 10X Reaction Buffer
- Tested for the absence of DNases and RNases
Avoiding RNase Contamination
Moloney Murine Leukemia Virus (M-MuLV, MMLV) Reverse Transcriptase is an RNA-directed DNA polymerase. This enzyme can synthesize a complementary DNA strand initiating from a primer using either RNA (cDNA synthesis) or single-stranded DNA as a template. (1-4). M-MuLV Reverse Transcriptase lacks 3´ → 5´ exonuclease activity.
Product SourceThe gene encoding M-MuLV Reverse Transcriptase is expressed in E.coli in a vector that results in 16 additional amino acids at the N-terminus and 13 amino acids at the C-terminus. This construct results in a fully functional Reverse Transcriptase protein with a functional RNase H domain.
The following reagents are supplied with this product:
Store at (°C) Concentration M-MuLV Reverse Transcriptase Reaction Buffer -20 10 X
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- Reverse Transcriptases & RT-PCR,
- cDNA Synthesis & Reverse Transcriptases
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