Taq DNA Ligase
- Isolated from a recombinant source
- Thermostable ligase for incorporation of phosphorylated oligonucleotides during PCR and Ligase Chain Reaction
- Taq DNA Ligase is NOT a substitute for T4 DNA Ligase
- Supplied with 10X Reaction Buffer containing NAD+
- Not sure which ligase to choose? Refer to our DNA and RNA Ligase Properties Chart
Avoiding RNase Contamination
Taq DNA Ligase is a thermostable ligase that catalyzes the formation of a phosphodiester bond between the 5´-phosphate and the 3´-hydroxyl of two adjacent DNA strands. The strands to be ligated need to be hybridized and accurately paired, with no gap, to a complementary DNA strand; allowing resolution of single nucleotide variants. Taq DNA Ligase uses NAD as a cofactor and it is active at elevated temperatures (37° C – 75° C).
Product SourcePurified from an E. coli strain containing the cloned ligase gene from Thermus thermophilus HB8 (1-3).
The following reagents are supplied with this product:
Store at (°C) Concentration Taq DNA Ligase Reaction Buffer -20 10 X
* Reagents' Storage Notes
- For long term storage (>30 days), store at -80°C.
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