Taq DNA Ligase
- Isolated from a recombinant source
- Thermostable ligase for incorporation of phosphorylated oligonucleotides during PCR and Ligase Chain Reaction
- Taq DNA Ligase is NOT a substitute for T4 DNA Ligase
- Supplied with 10X Reaction Buffer containing NAD+
- Not sure which ligase to choose? Refer to our DNA and RNA Ligase Properties Chart
DNA Ligase Fidelity: when does it matter?
Ordering InformationDid you know this product can be customized or purchased in larger volumes? Submit an inquiry to find out more about customization options.
Taq DNA Ligase is a thermostable ligase that catalyzes the formation of a phosphodiester bond between the 5´-phosphate and the 3´-hydroxyl of two adjacent DNA strands. The strands to be ligated need to be hybridized and accurately paired, with no gap, to a complementary DNA strand; allowing resolution of single nucleotide variants. Taq DNA Ligase uses NAD as a cofactor and it is active at elevated temperatures (37° C – 75° C).
Product SourcePurified from an E. coli strain containing the cloned ligase gene from Thermus thermophilus HB8 (1-3).
The following reagents are supplied with this product:
Store at (°C) Concentration Taq DNA Ligase Reaction Buffer -20 10 X
* Reagents' Storage Notes
- For long term storage (>30 days), store at -80°C.
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