T4 DNA Polymerase
T4 DNA Polymerase catalyzes the synthesis of DNA in the 5´→ 3´ direction and requires the presence of template and primer.
- Gap filling (no strand displacement activity)
- Removal of 3’ overhangs or fill-in of 5’ overhangs to form blunt ends
- Lacks 5’ —> 3’ exonuclease activity
- Probe labeling using replacement synthesis
- Singe-strand deletion subcloning
DNA Blunting Tutorial
Traditional Cloning Workflow
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T4 DNA Polymerase catalyzes the synthesis of DNA in the 5´→ 3´ direction and requires the presence of template and primer. This enzyme has a 3´→ 5´ exonuclease activity which is much more active than that found in DNA Polymerase I (E. coli). Unlike E. coli DNA Polymerase I, T4 DNA Polymerase does not have a 5´→ 3´ exonuclease function.
Product SourcePurified from a strain of E. coli that carries the T4 DNA Polymerase gene.
The following reagents are supplied with this product:
Store at (°C) Concentration NEBuffer™ 2.1 -20 10 X
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