Accurate quantitation of next-generation sequencing (NGS) libraries is essential for maximizing data output and quality from each sequencing run. For Illumina® sequencing specifically, accurate quantitation of libraries is critical to achieve optimal cluster densities, a requirement for optimal sequence output. qPCR is considered to be the most accurate and effective method of library quantitation, providing considerably higher consistency and reproducibility of quantitation. Further, amplification-based methods quantitate only those molecules that contain both adaptor sequences, thereby providing a more accurate estimate of the concentration of the library molecules that can be sequenced. The NEBNext® Library Quant Kit has been optimized to provide substantial performance and workflow improvements to qPCR-based library quantitation.
The following reagents are supplied with this product:
|Store at (°C)||Concentration|
|NEBNext Library Quant Master Mix||-20|
|NEBNext Library Quant Primer Mix||-20|
|NEBNext® Library Dilution Buffer||-20||10 X|
|NEBNext Library Quant DNA Standard 1|
|NEBNext Library Quant DNA Standard 2||-20|
|NEBNext Library Quant DNA Standard 3||-20|
|NEBNext Library Quant DNA Standard 4||-20|
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