NEBNext^® Library Quant Kit for Illumina^®

Features

• Provides more accurate and reproducible quant values than alternative methods and kits
• Compatible with libraries with a broad range of insert sizes and GC content, made by a variety of methods
• Requires only 4 standards, allowing more libraries to be quantitated per kit 
• Supplied with a convenient, Library Dilution Buffer
• The NEBNext Library Quant Master Mix requires only the addition of primers
• Utilizes a single extension time for all libraries, regardless of insert size
• Library quant values can be easily calculated using NEB’s online tool, at NEBioCalculator.neb.com
• ROX is included in the kit, for use with qPCR instruments that require a reference dye for normalization

Companion Products

• NEBNext® Library Dilution Buffer

Materials Sold Separately

• NEBNext® Library Dilution Buffer

1. Storage of Kit Components:
   All of the individual components of the NEBNext Library Quant Kit for Illumina are stable at –20°C for two years. When not in use, kit components should be stored at –20°C. All components of the kit, including the combined NEBNext Library Quant Master Mix and Primer Mix, are stable for at least 30 freeze/thaws.
   
   Once the Primer Mix has been added to the NEBNext Library Quant Master Mix (see Protocol on page 6 of manual), the resulting mix can be stored at –20°C for seven months. For short-term storage, this mix may be stored at 4°C for up to six weeks.
   
   Reactions can be prepared at room temperature, but unused portions of the kit reagents should be kept on ice after thawing them for use.
   
   
2. For Reagents Supplied:
   
   If you need dilution buffer in addition to what is provided in the kit, it can be purchased separately.

1. NEBNext Library Quant Kit Quick Protocol (E7630)
2. Experimental Considerations - NEBNext Library Quant Kit (E7630)
3. NEBNext Library Quant Kit Protocol - NEBNext Library Quant Kit (E7630)
4. Data Analysis - NEBNext Library Quant Kit (E7630)
5. Expected Results - NEBNext Library Quant Kit (E7630)

• Improved library quantitation for a broad range of library types E7630

1. What are the advantages of the qPCR-based NEBNext Library Quant Kit compared to other methods of quantitating NGS libraries?
2. What libraries can be quantitated with the Quant Kit?
3. How much should I dilute my library to run in the Quant Kit?
4. Can I run the NEBNext Library Quant Kit on my qPCR machine?
5. Which ROX should I use for my qPCR machine?
6. Are 4 standards enough for a qPCR quantitation?
7. Does the library quant require a size correction?
8. What other materials/information do I need to run the kit?
9. How many libraries can I quantitate at a time?
10. How long is a run of the Quant Kit?
11. Do you provide any analysis tools to help with the quantitation?
12. Can I see adaptor dimers as a measure of library quality?
13. How long can I store the master mix after adding the primers?
14. Can the NEBNext Library Dilution Buffer be purchased separately?

Product Citation Tool

• The Quantitation Question: How does accurate library quantitation influence sequencing?

Quality Control tests are performed on each new lot of NEB product to meet the specifications designated for it. Specifications and individual lot data from the tests that are performed for this particular product can be found and downloaded on the Product Specification Sheet, Certificate of Analysis, data card or product manual. Further information regarding NEB product quality can be found here.

The Specification sheet is a document that includes the storage temperature, shelf life and the specifications designated for the product. The following file naming structure is used to name these document files: [Product Number]_[Size]_[Version]

• E7630S_L_v1

The Certificate of Analysis (COA) is a signed document that includes the storage temperature, expiration date and quality controls for an individual lot. The following file naming structure is used to name these document files: [Product Number]_[Size]_[Version]_[Lot Number]

• E7630L_v0_10010901
• E7630S_v0_10011217
• E7630S_v0_10013445
• E7630S_v1_10011217
• E7630L_v1_10016519
• E7630S_v1_10017620
• E7630L_v1_10019879
• E7630S_v1_10019924
• E7630L_v1_10023912
• E7630L_v1_10028725
• E7630S_v1_10026838
• E7630L_v1_10033111
• E7630S_v1_10032067
• E7630S_v1_10035302
• E7630L_v1_10036419
• E7630L_v1_10036443
• E7630L_v1_10039716
• E7630S_v1_10038647
• E7630L_v1_10027352
• E7630S_v1_10042942
• E7630L_v1_10045807
• E7630L_v1_10046598
• E7630S_v1_10047426
• E7630L_v0_10050075
• E7630S_v1_10050511
• E7630L_v1_10051827
• E7630L_v1_10042112
• E7630S_v1_10054799

The following is a list of Safety Data Sheet (SDS) that apply to this product to help you use it safely.

• NEBNext Library Quant Master Mix
• NEBNext Library Quant Primer Mix
• NEBNext® Library Dilution Buffer
• NEBNext Library Quant DNA Standard 1
• NEBNext Library Quant DNA Standard 2
• NEBNext Library Quant DNA Standard 3
• NEBNext Library Quant DNA Standard 4
• ROX (Low)
• ROX (High)

This product is covered by one or more patents, trademarks and/or copyrights owned or controlled by New England Biolabs, Inc (NEB).

While NEB develops and validates its products for various applications, the use of this product may require the buyer to obtain additional third party intellectual property rights for certain applications.

For more information about commercial rights, please contact NEB's Global Business Development team at [email protected].

This product is intended for research purposes only. This product is not intended to be used for therapeutic or diagnostic purposes in humans or animals.