The Quant Kit is compatible with all qPCR instruments tested thus far, using either standard or fast temperature ramping protocols. For machines that use a reference dye for normalization, please consult the kit manual or the FAQ on ROX for appropriate ROX selection. The Quant Kit has been run successfully on the following qPCR machines:
| Manufacturer | Instrument |
|---|---|
| Applied Biosystems® | StepOne™ |
| StepOnePlus™ | |
| Prism® 7000 | |
| Prism 7900HT | |
| 7500 Fast | |
| ViiA™ 7 | |
| Bio-Rad | iQ® 5 |
| CFX96™ | |
| CFX384™ | |
| Life Technologies | QuantStudio™ 7 |
| Roche | LightCycler® 96 |
| LightCycler 480 |
Please contact us at info@neb.com if you would like to discuss performance on any qPCR instrument not listed above.
