FAQ: What libraries can be quantitated with the Quant Kit?

Any library containing the standard Illumina P5 and P7 adaptor sequences can be quantitated with the kit. Libraries can be prepared using any desired method as long as these adaptor sequences are introduced. Quantitation with the Quant Kit has been successfully performed using a number of library construction kits, including those listed below:  

Manufacturer Library Prep Kit
New England Biolabs® NEBNext® Ultra™ II DNA
NEBNext Ultra II FS
NEBNext Enzymatic Methyl-seq (EM-seq™)
NEBNext Immune Sequencing
NEBNext Ultra II Directional RNA
NEBNext Ultra II RNA
NEBnext Single Cell/Low Input RNA
Clontech SMARTer® Stranded Total RNA
KAPA™ Hyper
Thermo Scientific® MuSeek
Illumina® Nextera Rapid Capture
ScriptSeq™ v2
TruSeq DNA PCR-free
TruSeq Nano®
TruSeq RNA v2
TruSeq RNA Access
TruSight® Rapid Capture
Illumina PCR-free
Takara Prep™ ILM DNA

A wide variety of input genomic material is compatible with the Quant Kit. Kit performance was validated using libraries ranging in size from 140–1000 bp and 20–70% GC. Input material can be sRNA, FFPE DNA, or gDNA libraries from any source of interest including GC extremes, e.g. Plasmodium and Rhodopseudomonas.