NEBNext® Enzymatic Methyl-seq Kit

For enzymatic fragmentation of DNA for EM-seq, NEBNext UltraShear (NEB #M7634) is now available.

NEBNext Enzymatic Methyl-seq (EM-seq™) is a new method for identification of 5-mC and 5-hmC.

While bisulfite sequencing has been the gold standard for methylome analysis, this conversion treatment damages DNA, resulting in fragmentation, loss and bias. 

In contrast, the highly effective enzyme-based conversion in the NEBNext Enzymatic Methyl-seq Kit minimizes damage to DNA and, with the supplied NEBNext Ultra II library preparation workflow reagents, produces high quality libraries that enable superior detection of 5-mC and 5-hmC from fewer sequencing reads.

  • Superior sensitivity of detection of 5-mC and 5-hmC 
  • Greater mapping efficiency
  • More uniform GC coverage 
  • Detection of more CpGs with fewer sequence reads
  • Uniform dinucleotide distribution
  • High-efficiency library preparation, with larger library insert sizes
  • Conversion module also available separately

Enzymatic fragmentation of DNA compatible with EM-seq workflows can be achieved using NEBNext UltraShear (NEB #M7634).

View or download extensive EM-seq performance data in our Technical Note.

For specific detection of 5hmC, the NEBNext Enzymatic E5hmC-seq Kit (NEB #E3350) is now also available.


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Catalog # Concentration Size List Price Your Price Quantity
E7120S Not Applicable 24 reactions $904.00
E7120L Not Applicable 96 reactions $3,394.00
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