PURExpress® In Vitro Protein Synthesis Kit
PURExpress® is a reconstituted protein synthesis system based on the PUREsystem™ (Shimizu et al., 2001) where all necessary components needed for in vitro transcription and translation are purified from E. coli.
- Defined system with all his-tagged proteins for coupled transcription/translation; Ribosome is not his-tagged
- Minimal nuclease and protease activity
- Protein of interest can be synthesized and visualized in a few hours
- Simple protocol and easy setup with Solutions A and B
- Templates can be either plasmid DNA, linear DNA or mRNA
- Synthesized protein can be co-translationally radiolabeled or fluorescently labeled
- Protein can be reverse-purified or subject to direct functional analysis
- Applications include high throughput screening/directed evolution, synthetic biology, toxic or difficult to express protein synthesis, studies on protein folding, activity and protein-protein interactions
- Due to reconstituted nature several kits are offered where translation factors or macromolecules have been omitted to facilitate specific studies (see companion products)
A rapid method for gene expression analysis, PURExpress® is a novel cell-free transcription/translation system reconstituted from the purified components necessary for E. coli translation. With minimal nuclease and protease activity, the PURExpress system preserves the integrity of DNA and RNA templates/complexes and results in proteins that are free of modification and degradation. Transcription and translation are carried out in a one-step reaction, and require the mixing of only two tubes. With results available in a few hours, PURExpress saves valuable laboratory time and is ideal for high throughput technologies.
The following reagents are supplied with this product:
Store at (°C) Concentration PURExpress Solution A -80 Control (DHFR) template (10 μl) -80 Solution B (75 μl) -80 3.3 X
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