Thanksgiving Shipping Schedule: Orders placed on Tuesday, November 26, 2019 will ship for delivery Wednesday, November 27, 2019.
Orders placed Wednesday, November 27, Thursday, November 28 and Friday, November 29, 2019 will be shipped on Monday, December 2, 2019.

NEBExpress® Cell-free E. coli Protein Synthesis System

The NEBExpress™ Cell-free E. coli Protein Synthesis System is an extract-based transcription/translation system derived from E. coli cells that have been engineered for high in vitro synthesis performance. It is designed to synthesize proteins ranging in size from 17-230 kDa in just 2-4 hours under the control of T7 RNA Polymerase.

  • Synthesize high yields of protein (typically 0.5 mg/ml) 
  • Protein can be synthesized and visualized in approximately 2 - 4 hours
  • Synthesize various target proteins ranging from 17 to 230 kDa
  • Templates can be plasmid DNA, linear DNA or mRNA.
  • Minimal protease activity ensures stability of desired target protein
  • RNase contamination can be inhibited by the supplied RNase inhibitor, eliminating clean-up steps
  • Protein can be isolated with affinity purification or subject to direct functional analysis
  • Reactions can be directly loaded onto an SDS-PAGE gel without the need for acetone or TCA precipitation
  • Flexible reaction conditions achieve maximum yield; protein synthesis can be sustained for 10 hours at 37°C or up to 24 hours at lower temperatures
  • Reactions can be miniaturized or scaled up
  • Compatible with the PURExpress Disulfide Bond Enhancer (NEB #E6820) and NEBExpress GamS Nuclease Inhibitor (NEB #P0774)
Learn more about how this product is being used in the Product Citation Tool
Protein Expression Special Offer
Catalog # Concentration Size List Price Your Price Quantity
E5360S Not Applicable 10 reactions $210.00
E5360L Not Applicable 100 reactions $1,700.00
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