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PURExpress® Δ Ribosome Kit

Ordering Information

  • 10 reactions
    $328.00
  • Product Information
    A rapid method for gene expression analysis, PURExpress® is a novel cell-free transcription/translation system reconstituted from the purified components necessary for E. coli translation. The relative nuclease-free and protease-free nature of the PURExpress platform preserves the integrity of DNA and RNA templates/ complexes and results in proteins that are free of modification and degradation. Transcription and translation are carried out in a one-step reaction, and require the mixing of only two tubes. With results available in a few hours, PURExpress saves valuable laboratory time and is ideal for high throughput technologies

    PURExpress Citations


    Figure 1: Protein expression using the PURExpress® In Vitro Protein Synthesis Kit Figure 1: Protein expression using the PURExpress® In Vitro Protein Synthesis Kit.
    25 μl reactions containing 250 ng template DNA and 20 units RNase Inhibitor were incubated at 37°C for 2 hours. 2.5 μl of each reaction was analyzed by SDS-PAGE using a 10–20% Tris-glycine gel. The red dot indicates the protein of interest. Marker M is the Protein Ladder (NEB #P7703 ).
    Figure 2: Incorporation of 35S-methionine enables visualizationof protein by autoradiography Figure 2: Incorporation of 35S-methionine enables visualizationof protein by autoradiography
    25 μl reactions containing 250 ng template DNA, 20 units RNase Inhibitor and 2 μl 35S-met were incubated at 37°C for 2 hours. 2.5 μl of each reaction was analyzed by SDS-PAGE, the gel was fixed for 10 minutes, dried for 2 hours at 80°C and exposed to x-ray film for 5 hours at -80°C.
    Figure 3: Schematic diagram of protein synthesis and purification by PURExpressFigure 3: Schematic diagram of protein synthesis and purification by PURExpress

    Figure 4: Expression and reverse purification of DHFR (A) and T4 DNA Ligase (B) using PURExpress Figure 4: Expression and reverse purification of DHFR (A) and T4 DNA Ligase (B) using PURExpress
    125 μl reactions were carried out according to recommendations in the accompanying manual. Samples were analyzed on a 10–20% Tris-glycine gel and stained with Coomassie Blue. Note that in both cases, the desired protein can be visualized in the total protein fraction. The red dot indicates the protein of interest. Marker M is the Protein Ladder (NEB #P7703 ).

    Highlights

    • Cleaner System - sample degradation eliminated
    • Easy-to-use - protein expression complete in approximately two hours
    • Simple Analysis - protein can often be visualized directly on a Coomassie stained gel

    Kit Components

    The following reagents are supplied with this product:

    Store at (°C) Concentration
    PURExpress Solution A -80
    Control (DHFR) template (10 μl) -80
    Factor Mix (30 μl) -80
    E. coli Ribosome -80
    Product Categories:
    Cell-Free Expression Products
    Applications:
    Cell-Free Protein Expression
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