HiScribe™ SP6 RNA Synthesis Kit

  • Generate over 80 μg RNA per reaction
  • Enables full substitution of NTPs for labeling and incorporation of modified bases
  • Template removal and mRNA purification reagents included
  • Linearized control template included for verification of RNA synthesis

Ordering Information

E2070S_pdp_800x450
  • 50 reactions
    $270.00
  • Product Information
    The HiScribe SP6 RNA Synthesis Kit is designed for the in vitro transcription of RNA using SP6 RNA Polymerase. This kit is suitable for synthesis of high yield RNA transcripts and for incorporation of cap analogs (not included) or modified nucleotides (not included) to obtain capped, biotin-labeled or dye-labeled RNA. The kit is also capable of synthesizing high specific activity radiolabeled RNA for use as probes or targets.

    RNA synthesized from this kit is suitable for many applications including RNA structure and function studies, ribozyme biochemistry, probes for RNase protection or gel shift assays, hybridization-based blots, anti-sense RNA or RNAi experiments, microarray analysis, microinjection, sgRNA synthesis and in vitro translation studies.

    This kit contains sufficient reagents for 50 reactions of 25 µl each. Each standard reaction yields ≥ 80 µg of RNA from 1 µg SP6 Control Template DNA. Each kit can yield ≥ 4 mg of RNA.

    Figure 1: Transcription by SP6 RNA Polymerase.

    Figure 1 Transcription by SP6 RNA Polymerase
    Figure 2: Comparison of RNA yields from commercially-available SP6 in vitro transcription kits demonstrates the robustness of the NEB HiScribe SP6 RNA Synthesis Kit

    NEB HiScribe SP6 RNA Synthesis Kit: Comparison of RNA yields
    Reactions with 1 μg linearized plasmid DNA templates were incubated at 37°C in a PCR machine according to the manufacturer’s recommendations. Transcripts were treated with DNase I, purified by LiCl precipitation and quantified using a NanoDrop™ Spectrophotometer. The HiScribe SP6 RNA Synthesis Kit from NEB is faster and achieves higher yields for RNAs of various lengths.

    Kit Components

    The following reagents are supplied with this product:

    Store at (°C) Concentration
    LiCl Solution -20
    SP6 Reaction Buffer -20 10 X
    DNase I (RNase-free) -20 2 units/μl
    SP6 RNA Polymerase Mix -20
    SP6 Control Template -20 0.25 μg/μl
    ATP (Tris) -20 50 mM
    GTP (Tris) -20 50 mM
    UTP (Tris) -20 50 mM
    CTP (Tris) -20 50 mM
    Product Categories:
    RNA Synthesis
    Applications:
    RNA Labeling,
    In vitro Synthesis (IVT)
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