The nucleotide sequence files available below are those used to produce the plasmid vector, viral and bacteriophage maps contained in New England Biolabs Catalog as well as the tables containing the locations of sites. Maps and location of sites are PDF files. Sequence files are in FASTA or/and GenBank format. Detailed frequencies with which restriction endonuclease sites occur in commonly used DNA molecules can be found on Frequencies of Restriction Sites.
DNASU is a central repository for plasmid clones and collections that may also be helpful.
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The Q5® Site-Directed Mutagenesis Kit enables rapid, site-specific mutagenesis of double-stranded plasmid DNA in less than 2 hours.
The Gibson Assembly™ Cloning Kit is a robust method for assembling 1-2 DNA fragments, with a cloning vector, and ensuring high transformation efficiencies - all in a little less than 2 hours.