DNA Sequences and Maps Tool

The nucleotide sequence files available below are those used to produce the plasmid vector, viral and bacteriophage maps contained in New England Biolabs Catalog as well as the tables containing the locations of sites. Maps and location of sites are PDF files. Sequence files are in FASTA or/and GenBank format. Detailed frequencies with which restriction endonuclease sites occur in commonly used DNA molecules can be found on Frequencies of Restriction Sites.

DNASU is a central repository for plasmid clones and collections that may also be helpful.

Vectors Vector Set Nucleotide Sequences Genbank Link Length (bp) Maps (pdf)
Adenovirus-2     GenBank 35937 Map
DHFR Control Template PURExpress Fasta GenBank 2727  
FLuc Control Plasmid T7 High Yield
Fasta GenBank 3925
Lambda   Fasta GenBank 48502 Map
Lambda_gt11(SacI-KpnI)   Fasta GenBank 1988 Map
LITMUS-U USER Fasta GenBank 2715  
LITMUS28 LITMUS Fasta GenBank 2823  
LITMUS28i LITMUS Fasta GenBank 2823 Map
LITMUS28i-Mal LITMUS Fasta GenBank 3593  
LITMUS29 LITMUS Fasta GenBank 2820  
LITMUS38 LITMUS Fasta GenBank 2814  
LITMUS38i LITMUS Fasta GenBank 2814 Map
LITMUS39 LITMUS Fasta GenBank 2817  
M13KE PhD Fasta GenBank 7222 Map
M13KO7   Fasta GenBank 8669  
M13mp/pUC         Map
M13mp18   Fasta GenBank 7249 Map
NEBExpress Control DHFR-His plasmid   Fasta GenBank 2775  
pACP-ADRβ2 Cellular Imaging Fasta GenBank 6824  
pACP-GPI Cellular Imaging Fasta GenBank 5238  
pACP-tag(m) Cellular Imaging Fasta GenBank 5088  
pACP-tag(m)-2 Cellular Imaging Fasta GenBank 5543  
pACYC177   Fasta GenBank 3941 Map
pACYC184   Fasta GenBank 4245 Map
pBeloBAC11   Fasta GenBank 7507 Map
pBR322   Fasta GenBank 4361 Map
pCLIP-H2B Cellular Imaging Fasta GenBank 6183  
pCLIP-NK1R Cellular Imaging Fasta GenBank 7128  
pCLIP-tag(m) Cellular Imaging Fasta GenBank 5805  
pCLIPf Cellular Imaging Fasta GenBank 5862  
pCLIPf-Cox8A Cellular Imaging Fasta GenBank 6077  
pCLIPf-H2B Cellular Imaging Fasta GenBank 6249  
pCLIPf-NK1R Cellular Imaging Fasta GenBank 7128  
pCLuc Mini-TK 2   Fasta GenBank 6131  
pCLuc-Basic 2 Luciferase Fasta GenBank 6061  
pCMV-CLuc 2   Fasta GenBank 6905  
pCMV-GLuc Luciferase Fasta GenBank 5764 Map
pCMV-GLuc 2 Luciferase Fasta GenBank 5764 Map
pCYB1 IMPACT Fasta GenBank 6846  
pCYB2 IMPACT Fasta GenBank 6843  
pCYB3 IMPACT Fasta GenBank 6846  
pCYB4 IMPACT Fasta GenBank 6843  
pET11c pET Fasta GenBank 5672 Map
pFOS1   Fasta GenBank 9738 Map
pGGA   Fasta GenBank 2174 Map
pGGAselect   Fasta GenBank 2220 Map
pGLuc Mini-TK Luciferase Fasta GenBank 4990 Map
pGLuc Mini-TK 2 Luciferase Fasta GenBank 5028 Map
pGLuc-Basic Luciferase Fasta GenBank 4920 Map
pGLuc-Basic 2 Luciferase Fasta GenBank 4958 Map
pGPS1.1 GPS Fasta GenBank 4814 Map
pGPS2.1 GPS Fasta GenBank 4490 Map
pGPS3 GPS Fasta GenBank 4293 Map
pGPS4 GPS Fasta GenBank 3899 Map
pGPS5 GPS Fasta GenBank 4223 Map
pGT-N28 pGT Fasta GenBank 4665  
pGT-N29 pGT Fasta GenBank 4662  
pGT-N38 pGT Fasta GenBank 4656  
pGT-N39 pGT Fasta GenBank 4647  
phiX174     GenBank 5386 Map
pKLAC-malE Yeast Fasta GenBank 10153  
pKLAC1 Yeast Fasta GenBank 9091 Map
pKLAC1-GLuc Yeast Fasta GenBank 9589 Map
pKLAC2 Yeast Fasta GenBank 9107 Map
pKLMF-EK Yeast Fasta GenBank 9991 Map
pKLMF-FX Yeast Fasta GenBank 9988 Map
pKYB1 IMPACT Fasta GenBank 8393  
pMAL-c pMAL Fasta GenBank 6146  
pMAL-c2 pMAL Fasta GenBank 6644  
pMAL-c2E pMAL Fasta GenBank 6649  
pMAL-c2G pMAL Fasta GenBank 6649  
pMAL-c2X pMAL Fasta GenBank 6646  
pMAL-c4E pMAL Fasta GenBank 6648  
pMAL-c4G pMAL Fasta GenBank 6648  
pMAL-c4X pMAL Fasta GenBank 6645  
pMAL-c5E pMAL Fasta GenBank 5680  
pMAL-c5G pMAL Fasta GenBank 5680  
pMAL-c5X pMAL Fasta GenBank 5677  
pMAL-c5X-His pMAL Fasta GenBank 5695  
pMAL-c6T pMAL Fasta GenBank 5247  Map
pMAL-cRI pMAL Fasta GenBank 6131  
pMAL-p pMAL Fasta GenBank 6221  
pMAL-p2 pMAL Fasta GenBank 6719  
pMAL-p2E pMAL Fasta GenBank 6724  
pMAL-p2G pMAL Fasta GenBank 6724  
pMAL-p2X pMAL Fasta GenBank 6721 Map
pMAL-p4E pMAL Fasta GenBank 6723  
pMAL-p4G pMAL Fasta GenBank 6723  
pMAL-p4X pMAL Fasta GenBank 6720 Map
pMAL-p5E pMAL Fasta GenBank 5755 Map
pMAL-p5G pMAL Fasta GenBank 5755 Map
pMAL-p5X pMAL Fasta GenBank 5752 Map
pMAL-pIII PhD Fasta GenBank 6706 Map
pMCP-GPI Cellular Imaging Fasta GenBank 5238  
pMCP-tag(m) Cellular Imaging Fasta GenBank 5088  
pMiniT PCR Cloning Kit Fasta GenBank 2525 Map
pMiniT2.0 PCR Cloning Kit Fasta GenBank 2588 Map
pMXB10 IMPACT Fasta GenBank 7813 Map
pMYB5 IMPACT Fasta GenBank 8602  
pNEB193   Fasta GenBank 2713 Map
pNEB205a USER Fasta GenBank 2710 Map
pNEB206a USER Fasta GenBank 2722 Map
pNEBR-R1 RheoSwitch Fasta GenBank 10338  
pNEBR-R1Puro RheoSwitch Fasta GenBank 9337  
pNEBR-X1 RheoSwitch Fasta GenBank 4287 Map
pNEBR-X1GLuc RheoSwitch Fasta GenBank 4821 Map
pNEBR-X1Hygro RheoSwitch Fasta GenBank 6286 Map
pRS413 Yeast Fasta GenBank 4970 Map
pRS414 Yeast Fasta GenBank 4788 Map
pRS415 Yeast Fasta GenBank 6021 Map
pRS416 Yeast Fasta GenBank 4891 Map
pSNAP-ADRB2 Cellular Imaging Fasta GenBank 7122  
pSNAP-CaaX Control Plasmid Cellular Imaging Fasta GenBank 5418 Map
pSNAP-Cox8A Control Plasmid Cellular Imaging Fasta GenBank 6015 Map
pSNAP-H2B Cellular Imaging Fasta GenBank 6183  
pSNAP-tag(m) Cellular Imaging Fasta GenBank 5802 Map
pSNAP-tag(m)2 Cellular Imaging Fasta GenBank 5849  
pSNAP-tag(T7) Cellular Imaging Fasta GenBank 3725  
pSNAP-tag(T7)2 Cellular Imaging Fasta GenBank 6495  
pSNApf Cellular Imaging Fasta GenBank 5849 Map
pSNAPf-ADRβ2 Cellular Imaging Fasta GenBank 7164  
pSNAPf-Cox8A Cellular Imaging Fasta GenBank 6064  
pSNAPf-H2B Cellular Imaging Fasta GenBank 6236  
pSV40-CLuc Luciferase Fasta GenBank 5256  
pSV40-GLuc Luciferase Fasta GenBank 4178 Map
pTK-CLuc   Fasta GenBank 7000  
pTK-GLuc Luciferase Fasta GenBank 5897 Map
pTWIN-MBP1 IMPACT Fasta GenBank 8515  
pTWIN1 IMPACT Fasta GenBank 7375 Map
pTWIN2 IMPACT Fasta GenBank 7192  
pTXB1 IMPACT Fasta GenBank 6706 Map
pTXB3 IMPACT Fasta GenBank 6906 Map
pTYB1 IMPACT Fasta GenBank 7477 Map
pTYB11 IMPACT Fasta GenBank 7412 Map
pTYB12 IMPACT Fasta GenBank 7415 Map
pTYB2 IMPACT Fasta GenBank 7474 Map
pTYB21 IMPACT Fasta GenBank 7514 Map
pTYB22 IMPACT Fasta GenBank 7511 Map
pTYB3 IMPACT Fasta GenBank 7477 Map
pTYB4 IMPACT Fasta GenBank 7474 Map
pUC19   Fasta GenBank 2686 Map
PURExpress Univ Primer PURExpress Fasta GenBank 85  
SV40     GenBank 5243 Map
T7     GenBank 39937 Map
YEp24 Yeast Fasta GenBank 7769 Map
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