FAQ: What is the promoter sequence for SP6 RNA Polymerase?

The SP6 promoter sequence is 5´ ATTTAGGTGACACTATAG 3´. SP6 RNA Polymerase starts transcription at the underlined G in the double-stranded promoter sequence. The polymerase then transcribes using the opposite strand as a template in the 5´ to 3´ direction. The first base in the transcript will be a G.