EpiMark® N6-Methyladenosine Enrichment Kit

The Epimark® N6-Methyladenosine Enrichment Kit can be used to enrich m6A modified RNA in immunoprecipitation protocols for downstream analysis by next-generation RNA sequencing or RT-qPCR.

  • Complete protocol for enrichment of m6A-modified –RNA and analysis by RT- qPCR included
  • RNA controls (m6A modified and unmodified RNA) enable monitoring of enrichment and depletion
  • Antibody supplied in a ready-to-use solution form
Catalog # Concentration Size List Price Your Price Quantity
E1610S Not Applicable 20 reactions $422.00
Please enter a quantity for at least one size
  • Product Information
    The EpiMark N6-Methyladenosine Enrichment Kit contains a rabbit monoclonal antibody specific for N6-Methyladenosine (m6A). The kit also contains two control RNAs, one with m6A modification (Gaussia luciferase) and one without (Cypridina luciferase) to monitor enrichment and depletion. The GLuc RNA control was transcribed in the presence of 20% m6ATP and 80% ATP.
    This kit can be used to enrich m6A modified RNA in immunoprecipitation protocols for downstream analysis by next-generation RNA sequencing or RT-qPCR. Modified RNA is isolated from a fragmented RNA sample by binding to the N6-Methyladenosine antibody attached to Protein G Magnetic Beads. After multiple wash and clean-up steps, the enriched RNA is eluted in nuclease-free water and is ready for further analysis.

    Epimark N6-Methyladenosine Enrichment Kit Workflow
    Figure 1. Workflow

    Each kit contains sufficient reagents to perform 10 x 2-round immunoprecipitations with starting amounts of up to 250 µg of ribosome depleted or poly A+ purified RNA. It contains sufficient reagents for 5 x 2-round immunoprecipitations when using up to 250 µg of total RNA.

    Kit Components

    The following reagents are supplied with this product:

    Store at (°C) Concentration
    N6-Methyladenosine Antibody -20
    m6A Control RNA -20 100 nM
    Unmodified Control RNA -20 100 nM
    Product Categories:
    RNA Isolation & Enrichment Products,
    RNA Reagents Products
    Applications:
    RNA Purification and Isolation,
    RNA Analysis
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