Protein G Magnetic Beads

Description

Protein G Magnetic Beads are an affinity matrix for the small-scale isolation and purification of immunoglobulins. A truncated form of recombinant Protein G is covalently coupled to a nonporous superparamagnetic particle. Protein G exhibits high affinity for subclasses of IgG from many species including human, rabbit, mouse, rat and sheep (1). In addition, Protein G can be used for immunoprecipitations with mouse monoclonal antibodies (1). The protein is coupled through a linkage that is stable and leak resistant over a wide pH range. This permits the immunomagnetic purification of IgGs from ascites, serum or cell culture supernatants; the matrix can then be regenerated without loss of binding capacity. Protein G Magnetic Beads can be used to immunoprecipitate target proteins from crude cell lysates using selected primary antibody. In addition, specific antibodies can be chemically cross-linked to the Protein G coated surface to create a reusable immunoprecipitation bead, avoiding the co-elution of antibody with target antigen (2,3).

Particles are supplied as a 1 ml suspension in PBS Buffer (pH 7.4), containing 0.1% BSA, 0.05% Tween® 20 and 0.05% NaN3.

Highlights

  • Small-scale purification or immunoprecipitation of IgG species
  • No centrifugation required
  • Regenerate matrix without binding capacity loss

Advantages and Features

Features

  • No centrifugation required; matrix can be regenerated without loss of binding capacity.
  • Minimal sample loss during pipetting because magnetic beads concentrate at the side of the tube instead of the bottom.
  • Protein A and Protein G magnetic beads allow for the small-scale purification or immunoprecipitation of IgG species.

Properties and Usage

Storage Temperature

4°C

Storage Conditions

0.02% sodium azide
0.1% BSA
0.05% Tween® 20
1X PBS
pH 7.4 @ 25°C

Binding Capacity

1 ml of Protein G Magnetic Beads binds >280 µg of Human IgG.

Can be Regenerated

yes

Notes

  1. Support Matrix: 1 ml of Protein G Magnetic Beads binds >280 µg of Human IgG.
  2. Affinity of Protein A/G for IgG Types from Different Species

References

  1. Harlow, E. and Lane, D. (1988). Bacterial Cell Wall Proteins that Bind Antibodies. Antibodies: A Laboratory Manual. 615-619.
  2. Schneider, C., Newman, R.A. et al. (1982). J. Biol. Chem.. 257, 10766.
  3. Sisson, T.H. and Castor, C.W. (1990). J. Immunol. Methods. 127, 215.

Protocols

  1. Purification of IgG using Protein A/G Magnetic Beads
  2. Immunoprecipitation using Protein A/G Magnetic Beads
  3. Cross-linking of IgG to Protein A or G Beads
  4. Phage Display: Solution-phase Panning with Affinity Bead Capture

Datacards

The Product Summary Sheet, or Data Card, includes details for how to use the product, as well as details of its formulation and quality controls. The following file naming structure is used to name the majority of these document files: [Catalog Number]Datasheet-Lot[Lot Number]. For those product lots not listed below, please contact NEB at info@neb.com or fill out the Technical Support Form for appropriate document.

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Safety Data Sheet

The following is a list of Safety Data Sheet (SDS) that apply to this product to help you use it safely.

Datacards

The Product Summary Sheet, or Data Card, includes details for how to use the product, as well as details of its formulation and quality controls. The following file naming structure is used to name the majority of these document files: [Catalog Number]Datasheet-Lot[Lot Number]. For those product lots not listed below, please contact NEB at info@neb.com or fill out the Technical Support Form for appropriate document.