For enzymatic fragmentation and library preparation of FFPE DNA samples the NEBNext UltraShear FFPE DNA Library Prep Kit (NEB #E6655) is now available.
Enzymatic fragmentation of DNA as part of the library prep workflow provides many advantages compared to mechanical shearing. However, specialized fragmentation reagents are required for enzymatic shearing in order to maintain methylation marks on samples for methylome analysis or for use with challenging samples such as FFPE DNA.
NEBNext UltraShear™ is a mix of enzymes that has been designed and optimized to fragment these sample types upstream of library preparation. This improves library quality and allows retention of methylation marks.
Enzymatic methods for DNA fragmentation in NGS workflows enable streamlined protocols, improved performance and scalability. However, specialized fragmentation reagents are required for samples for methylation analysis, to ensure that methylation marks are not removed, and for FFPE DNA. NEBNext UltraShear is a novel enzyme mix designed for fragmentation of these sample types that has a fast workflow and improves library preparation and sequencing metrics for DNA methylation studies and FFPE DNA.
NEBNext UltraShear is compatible with NEBNext Enzymatic Methyl-seq (EM-seq) (NEB #E7120).
NEBNext UltraShear in FFPE DNA sequencing workflows:
Improved usable reads
Lower artificial mutation frequency
NEBNext UltraShear for methylated DNA sequencing e.g., EM-seq
Higher library yields
Improved sequencing metrics
Improved CpG Coverage
Note that for high quality genomic DNA library prep with enzymatic fragmentation, we recommend NEBNext Ultra II FS DNA Library Prep for Illumina (NEB #E7805, #E6177).
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