NEBNext UltraShear®

For enzymatic fragmentation and library preparation of FFPE DNA samples the NEBNext UltraShear FFPE DNA Library Prep Kit (NEB #E6655) is now available.

Enzymatic fragmentation of DNA as part of the library prep workflow provides many advantages compared to mechanical shearing. However, specialized fragmentation reagents are required for enzymatic shearing in order to maintain methylation marks on samples for methylome analysis or for use with challenging samples such as FFPE DNA.

NEBNext UltraShear® is a mix of enzymes that has been designed and optimized to fragment these sample types upstream of library preparation. This improves library quality and allows retention of methylation marks.

View or download extensive performance data in our Data Supplement.

  • Compatible with methylation analysis workflows, including NEBNext® Enzymatic Methyl-seq (EM-seq) (NEB #E7120)
  • Compatible with FFPE DNA
  • Fast workflow with minimal hands-on time
  • For methylation analysis, improves library yields, CpG coverage and sequencing metrics
  • For FFPE DNA, increases usable reads and coverage uniformity and decreases artificial mutation frequency

For enzymatic fragmentation and library preparation of FFPE DNA samples we recommend the NEBNext UltraShear FFPE DNA Library Prep Kit (NEB #E6655)

Note: For library prep with enzymatic fragmentation, starting with high-quality genomic DNA samples, we recommend NEBNext Ultra™ II FS DNA Library Prep for Illumina® (NEB #E7805, #E6177) or NEBNext UltraExpress® FS DNA Library Prep Kit (NEB #3340).

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Catalog # Concentration Size List Price Your Price Quantity
M7634S Not Applicable 24 reactions $108.00
M7634L Not Applicable 96 reactions $432.00
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