T4 RNA Ligase 1 (ssRNA Ligase), High Concentration

The following reagents are supplied with this product:

NEB #	Component Name	Component #	Stored at (°C)	Amount	Concentration
M0437M     -20       T4 RNA Ligase 1 (ssRNA Ligase), High Concentration M0437MVIAL -20 1 x 0.167 ml 30,000 units/ml   T4 RNA Ligase Reaction Buffer B0216SVIAL -20 1 x 1.5 ml 10 X   50% PEG 8000 B1004SVIAL -20 2 x 1 ml 1 X   ATP N0437AVIAL -20 1 x 0.1 ml 100 mM

Application Features

• Ligation of ss-RNA and DNA
• Labeling of 3´-termini of RNA with 5´-[32P] pCp (3)
• Inter- and intramolecular joining of RNA and DNA molecules (4,5)
• Synthesis of single-stranded oligodeoxyribonucleotides (6)
• Incorporation of unnatural amino acids into proteins (7)

Companion Products

• T4 RNA Ligase 1 (ssRNA Ligase)

1. Addition of DMSO to 10% (v/v) isrequired for pCp ligation (3).

1. England, T., Gumport, R. and Uhlenbeck, O. (1977). Proc. Natl. Acad. Sci. USA. 74, 4839-4842.
2. Rand, K.N. and Gait, M.J. (1984). EMBO J. 3. 397-402.
3. England, T. and Uhlenbeck, O. (1978). Nature. 275, 560-562.
4. Romaniuk, P. and Uhlenbeck, O. (1983). In R. Wu, L. Grossman and K. Moldave(Ed.), Methods in Enzymology. Vol. 100, (pp. 52-56). New York: Academic Press.
5. Moore, M.J. and Sharp, P.A. (1992). Science. 256, 992-997.
6. Tessier, D.C., Brousseau, R. and Vernet, T. (1986). Anal. Biochem. 158, 171-178.
7. Noren, C.J. et al. (1989). Science. 244, 182-188.
8. Silber, R., Malathi, B.G. and Hurwitz, J. (1972). Proc. Natl. Acad. Sci. USA. 69, 3009-3013.

1. Ligation of an ssRNA-OH 3′ to a 5′ PO4-ssRNA/ssDNA adaptor using High Concentration T4 RNA Ligase 1 (NEB #M0437)

• Substrate-based Ligase Selection Chart

1. What is the optimal reaction temperature and time for T4 RNA Ligase I?
2. Can T4 RNA Ligase 1 be used to end label single stranded DNA or RNA?
3. Will T4 RNA Ligase 1 ligate double stranded DNA?
4. What is the most common cause of ligation failure when using T4 RNA Ligase 1?
5. Why doesn’t the new T4 RNA Ligase reaction buffer contain ATP?
6. Will T4 RNA Ligase 1 ligate single stranded DNA?
7. Will the ligation reaction using T4 RNA Ligase 1 produce circles or duplex products?
8. Can T4 RNA Ligase 1 be used to make single stranded RNA/DNA hybrids?
9. Can T4 RNA Ligase 1 ligate triphosphates?
10. Can T4 RNA Ligase 1 be used to add tails to linear duplex DNA?
11. Can a dideoxy be used to block ligation using T4 RNA Ligase 1?
12. Can T4 RNA Ligase 1 be used to incorporate unnatural amino acids into proteins?
13. Will PEG improve ligation with T4 RNA Ligase 1?
14. Is PEG 8000 available for purchase?

Quality Control tests are performed on each new lot of NEB product to meet the specifications designated for it. Specifications and individual lot data from the tests that are performed for this particular product can be found and downloaded on the Product Specification Sheet, Certificate of Analysis, data card or product manual. Further information regarding NEB product quality can be found here.

The Specification sheet is a document that includes the storage temperature, shelf life and the specifications designated for the product. The following file naming structure is used to name these document files: [Product Number]_[Size]_[Version]

• M0437M_v2

The Certificate of Analysis (COA) is a signed document that includes the storage temperature, expiration date and quality controls for an individual lot. The following file naming structure is used to name these document files: [Product Number]_[Size]_[Version]_[Lot Number]

• M0437M_v1_10009897
• M0437M_v1_10016477
• M0437M_v1_10031991
• M0437M_v1_10043599
• M0437M_v1_10061042
• M0437M_v1_10075389
• M0437M_v1_10094806
• M0437M_v1_10111301
• M0437M_v1_10122058
• M0437M_v1_10145054
• M0437M_v1_10159981
• M0437M_v1_10179327
• M0437M_v1_10218905
• M0437M_v1_10242813
• M0437M_v2_10266132
• M0437M_v2_10268389
• M0437M_v2_10270123
• M0437M_v2_10283867
• M0437M_v2_10317449
• M0437M_v2_10326339
• M0437M_v2_10339588

The following is a list of Safety Data Sheet (SDS) that apply to this product to help you use it safely.

• T4 RNA Ligase 1 (ssRNA Ligase), High Concentration
• T4 RNA Ligase Reaction Buffer
• PEG 8000

Products and content are covered by one or more patents, trademarks and/or copyrights owned or controlled by New England Biolabs, Inc (NEB). The use of trademark symbols does not necessarily indicate that the name is trademarked in the country where it is being read; it indicates where the content was originally developed. The use of this product may require the buyer to obtain additional third-party intellectual property rights for certain applications. For more information, please email busdev@neb.com.

This product is intended for research purposes only. This product is not intended to be used for therapeutic or diagnostic purposes in humans or animals.

New England Biolabs (NEB) is committed to practicing ethical science – we believe it is our job as researchers to ask the important questions that when answered will help preserve our quality of life and the world that we live in. However, this research should always be done in safe and ethical manner. Learn more.