Need assistance designing LAMP primers? Use the NEB LAMP Primer Design Tool.
This product is available in a glycerol-free format.
WarmStart RTx Reverse Transcriptase is a unique in silico designed RNA-directed DNA polymerase coupled with a reversibly-bound aptamer that inhibits RTx activity below 40°C. This enzyme can synthesize a complementary DNA strand initiating from a primer using RNA (cDNA synthesis) or single-stranded DNA as a template. RTx is a robust enzyme for RNA detection in amplification reactions and is particularly well suited for use in LAMP (loop-mediated isothermal amplification). The WarmStart property enables high throughput applications, room temperature setup, and increases the consistency and specificity of amplification reactions. RTx contains intact RNase H activity.
To support lyophilization, incorporation into microfluidic devices, and enable quick adoption into automation workflows, WarmStart RTx Reverse Transcriptase is also offered in a glycerol-free format.
Total RNA | WarmStart RTx | AMV RT |
100 ng | 3/3 | 3/3 |
10 ng | 3/3 | 3/3 |
1 ng | 3/3 | 3/3 |
100 pg | 3/3 | 2/3 |
10 pg | 3/3 | 2/3 |
1pg | 3/3 | 1/3 |
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