Typical RT-LAMP Protocol

1. Component	Final Conc./AMT.
   10X Isothermal Amplification Buffer	1X
   MgSO4 (100 mM)	6 mM (8 mM total)
   dNTP Mix (10 mM)	1.4 mM
   FIP/BIP Primers (25X)	1.6 µM
   F3/B3 Primers (25X)	0.2 µM
   LoopF/B Primers (25X)	0.4 µM
   Bst 2.0 or Bst 2.0 WarmStart DNA Polymerase	8 units
   WarmStart RTx Reverse Transcriptase	0.5 µl
   Total Reaction Volume	25 µl



2. Mix by vortexing.
   
   
3. Place directly at 65°C.