Kits for FFPE DNA library prep are now available, with or without enzymatic DNA fragmentation (NEBNext UltraShear™ FFPE DNA Library Prep Kit NEB #E6655 and NEBNext FFPE DNA Library Prep Kit NEB #E6650)
This new product further improves repair of FFPE DNA in next generation sequencing (NGS) workflows.
The methods used for fixation and storage of Formalin-Fixed, Paraffin-Embedded (FFPE) DNA samples cause significant damage, making it challenging to obtain high quality sequence data. The NEBNext FFPE DNA Repair v2 Module is an optimized cocktail of enzymes designed to repair FFPE DNA, and supplied with optimized reagents to enable a streamlined workflow for NGS library preparation.
The NEBNext FFPE DNA Repair v2 Module contains enzymes and buffers that have been optimized to repair FFPE DNA in next generation sequencing workflows in a streamlined manner.
The NEBNext FFPE DNA Repair v2 Module improves upon the performance of the original NEBNext FFPE DNA Repair Mix:
Table 1: Types of FFPE DNA damage and their ability to be repaired by the NEBNext FFPE DNA Repair v2 Module
|FFPE DAMAGE TYPE||REPAIRED BY THE FFPE DNA REPAIR v2 MODULE|
|Deamination of cytosine to uracil||Yes|
|Nicks and gaps||Yes|
|Blocked 3´ ends||Yes|
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