RNA Synthesis In vitro Transcription (IVT)
This chart outlines our portfolio to support RNA synthesis, including solutions for template generation, in vitro transcription, RNA capping, Poly(A) tailing, RNA purification, and nucleotide solutions for synthesizing modified mRNAs.
= available in GMP-grade
* “GMP Grade” and “GMP-grade” are branding terms NEB uses to describe products manufactured or finished at NEB’s Rowley facility. The Rowley facility was designed to manufacture products under more rigorous infrastructure and process controls to achieve more stringent product specifications and customer requirements. Products manufactured at NEB’s Rowley facility are manufactured in compliance with ISO 9001 and ISO 13485 quality management system standards. However, at this time, NEB does not manufacture or sell products known as Active Pharmaceutical Ingredients (APIs), nor does NEB manufacture its products in compliance with all of the Current Good Manufacturing Practice regulations.
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Nucleotide Solutions for RNA
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- A Typical DNase I Reaction Protocol (NEB #M0303)
- Capped RNA Synthesis using a Dinucleotide Cap Analog ARCA (NEB #E2040)
- Cap-0 synthesis using Vaccinia Capping Enzyme (NEB #M2080)
- DNA Template Preparation (E2040)
- High Specific Activity Radiolabeled RNA Probe Synthesis (NEB #E2040)
- A Typical Tailing Reaction (M0337)
- SP6 In Vitro Transcription Reaction Protocol (M0207)
- Standard RNA Synthesis with HiScribe T7 High Yield RNA Synthesis Kit (NEB #E2040)
- 2´-O-Methylation of Capped RNA (M0366)
- One-Step Cap-1 mRNA synthesis with Vaccinia Capping Enzyme (VCE) and mRNA Cap 2´-O-methyltransferase (2´-O-me) (NEB #M0366, #M2080)
- Labeling Protocol (M2080)
- In vitro digestion of DNA with Cas9 Nuclease, S. pyogenes (NEB #M0386)
- Poly(A) Tailing of RNA using E. coli Poly(A) Polymerase (NEB #M0276)
- Protocol for Standard RNA Synthesis (NEB #M0378)
- mRNA Synthesis with Modified Nucleotides (E2060)
- Capped mRNA Synthesis using Modified Nucleotides and the HiScribe® T7 ARCA mRNA Kit (NEB #E2065)
- Protocol for Standard RNA Synthesis
- Standard mRNA Synthesis (E2065)
- Using recombinant Cas9 nuclease to assess locus modification in genome editing experiments (NEB #M0386)
- Transfection of EnGen® Spy Cas9 HF1 (NEB #M0667) into adherent cells using the Lipofectamine® RNAiMAX System
- Protocol for Standard RNA Synthesis
- RNA Cap Analog Selection Chart
- Recommended HiScribe® RNA Synthesis Kits by Application
- RNA Polymerase Selection Chart
- RNA Ligase Selection Chart
- Avoiding Ribonuclease Contamination
Selection Tools
Usage Guidelines
- Wu, M.Z., Asahara, H., Tzertzinis, G., Roy, B. (2020) Synthesis of low immunogenicity RNA with high-temperature in vitro transcription RNA; PubMedID: 31900329
- Wulf, Madalee; Buswell, John; Chan, Siuhong; Dai, Nan; Marks, Katherine; Tzertzinis, George; Whipple, Joe; Correa, Ivan; Schildkraut, Ira; (2019) The yeast scavenger decapping enzyme DcpS and its application for in vitro RNA recapping Sci Rep; 9 (1), 8594. PubMedID: 31197197, DOI: 10.1038/s41598-019-45083-5
- Eyler, D.E., Franco, M.K., Batool, Z., Wu, M.Z., Dubuke, M.L., Dobosz-Bartoszek, M., Jones, J.D., Polikanov, Y.S., Roy, B., Koutmou, K.S (2019) Pseudouridinylatio of mRNA coding sequences alters translation Proc Natl Acad Sci U S A; 116(46), 23068-23074.. PubMedID: 31672910, DOI: 10.1073/pnas.1821754116
- Potapov, V., Fu, X., Dai, N., Correa, I.R., Jr., Tanner, N.A., Ong, J.L (2018) Base modificatons affecting RNA polymerase and reverse transcriptase fidelity Nucleic Acids Res; 46(11): 5753-5763, PubMedID: 29750267
Products and content are covered by one or more patents, trademarks and/or copyrights owned or controlled by New England Biolabs, Inc (NEB). The use of trademark symbols does not necessarily indicate that the name is trademarked in the country where it is being read; it indicates where the content was originally developed. All other trademarks are the property of their respective owners. The use of this product may require the buyer to obtain additional third-party intellectual property rights for certain applications. For more information, please email busdev@neb.com.
This product is intended for research purposes only. This product is not intended to be used for therapeutic or diagnostic purposes in humans or animals.
Interested in GMP-grade reagents for RNA Synthesis?
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Interested in GMP-grade reagents for RNA Synthesis?
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