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RNA Isolation & Enrichment

Studies that require the isolation of eukaryotic mRNA often take advantage of the polyadenine tail typically present at the 3’ end. One approach involves the use affinity matrices; poly-T oligonucleotides that hybridize to the complementary poly-A tails (1). Enrichment of miRNA and siRNAs can also be achieved using high-affinity binding proteins and the use of chitin magnetic beads (2, 3).

References:

  1. Aviv, H. and Leder, P. (1972) Proc. Natl. Acad. Sci. USA, 69, 1408. PMID: 4504350
  2. Silhavy, D. et al. (2002) EMBO J., 21, 3070-3080. PMID: 12065420
  3. Jin, J. et al. (2010) Biotechneques. 48, XVII-XXII. PMID: 20569217

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Protocols for RNA Isolation & Enrichment
RNA Ligase Selection Chart
NEB offers a variety of ligases for RNA research with unique specificities.
cDNA Synthesis Selection Chart
NEB offers several reagents for cDNA synthesis for use in applications including qPCR and qRT-PCR. For your convenience, reagents are available as kits or standalone products, depending on your needs.
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This product is intended for research purposes only. This product is not intended to be used for therapeutic or diagnostic purposes in humans or animals.


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