RNA Library Prep for Illumina

NEBNext has multiple library prep options to help you sequence all of your RNA samples.

Which way to RNA? 

• The fastest, easiest way to generate high-quality RNA libraries is to use the NEBNext UltraExpress RNA Library Prep Kit (NEB #E3330). With a 3-hour workflow, when combined with poly(A) mRNA enrichment or rRNA depletion kits, you can go from total RNA to high-quality directional libraries in a day. A single protocol, with the same adaptor concentration and number of PCR cycles, can be used for all samples in the 25-250 ng total RNA input range, and with the use of master mixes and fewer cleanups, you’ll see savings in tips and tubes as well as time.
• For input amounts that are outside of the 25-250 ng range, or are challenging (e.g., FFPE), the NEBNext Ultra II Directional RNA Kit is recommended. This tried and tested kit generates high-yield, high-quality strand-specific libraries from as little as 5 ng input.
• If non-stranded libraries are preferred, the NEBNext^® Ultra™ II RNA Library Prep Kit for Illumina^® (NEB #E7770) is available. 
• The adaptors and primers in NEBNext Multiplex Oligos enable simple yet powerful NGS sample multiplexing

For microRNAs and other small RNAs, our novel Small RNA workflow has been optimized to minimize adaptor-dimers, while producing high-yield, high-diversity libraries.

For single cell and ultra-low input RNA, our simple and robust workflow generates full length cDNAs, followed by conversion to sequence-ready libraries using the Ultra™ II FS workflow, that incorporates enzymatic DNA fragmentation.

NEBNext RNA library prep reagents are also available at scale for your high-throughput and/or development needs. Contact the Customized Solutions Team for more details on custom product options, large volumes, or OEM materials.