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  • NEBuffer Comparison Chart (1X)

    NEBuffer 1 NEBuffer 1.1
    yellow label yellow label
    10 mM Bis Tris Propane-HCl 10 mM Bis Tris Propane-HCl
    10 mM MgCl₂ 10 mM MgCl₂
    1 mM DTT (pH 7.0@25°C) 100 µg/ml BSA (pH 7.0@25°C)
    NEBuffer 2 NEBuffer 2.1
    blue label blue label
    10 mM Tris-HCl 10 mM Tris-HCl
    10 mM MgCl₂
    10 mM MgCl₂
    50 mM NaCl
    50 mM NaCl
    1 mM DTT (pH 7.9@25°C)
    100 µg/ml BSA (pH 7.9 @ 25°C)
    NEBuffer 3 NEBuffer 3.1
    red label red label
    50 mM Tris-HCl
    50 mM Tris-HCl
    10 mM MgCl₂
    10 mM MgCl₂
    100 mM NaCl
    100 mM NaCl
    1 mM DTT (pH 7.9@25°C)
    100 µg/ml BSA (pH 7.9 @ 25°C)
    NEBuffer 4 CutSmart Buffer
    green label green label
    20 mM Tris-acetate
    20 mM Tris-acetate
    10 mM Magnesium acetate
    10 mM Magnesium acetate
    50 mM Potassium acetate
    50 mM Potassium acetate
    1 mM DTT (pH 7.9@25°C)
    100 µg/ml BSA (pH 7.9 @ 25°C)