Automation for NEBNext® NGS Library Prep

Liquid-handling automation platforms are becoming ever-more essential for scalable, reproducible library preparation in next generation sequencing (NGS) workflows. The range of automation platforms continues to grow, so knowing where to look for support automating your library prep workflow can save both time and money. NEBNext library prep kits and reagents are designed with automation compatibility in mind, ensuring that your library quality doesn’t suffer as your throughput grows.

For support with any combination of NEBNext library prep kit and liquid-handling automation platform, please reach out to your local NEBNext Field Application Scientist with details about your needs.

Does NEB Support My Platform?

 
NEBNext closely cooperates with Automation Partners towards the development and optimization of automated methods that produce the highest quality sequencing data across supported liquid handling platforms. Methods are developed collaboratively according to best practices to be deployed and supported by Automation Partners globally.

Our team of automation Field Application Scientists work closely with all automation vendors to facilitate technical deployments of robust automated protocols.

NEBNext workflows are designed with automation in mind, featuring:

  • Reagent fill volume overages accommodate liquid handling platforms
  • Save time and reduce consumables waste with fewer bead clean-up steps
  • Modular kit components for workflow flexibility
  • Simple addition-only workflows eliminate lossy transfers
  • Expert field application support for reagents and instrumentation

Don’t see your automated method below? We are constantly developing new automated methods and are always happy to share progress on methods in development as well as to take on a new project!

Contemplating a new automation project? Please contact us for honest, expert consultation.

You can reach us at NEBNextAutomation@neb.com

NEBNext reagents have been automated on various liquid handling automation platforms, including:

  • Revvity
  • Beckman Coulter
  • SBT LabTech
  • Agilent
  • Hamilton
  • Tecan

Several applications have already been automated with NEBNext reagents, such as:

  • DNA-seq with Enzymatic Fragmentation
  • Directional RNA-seq
  • Enzymatic Methyl-seq (EM-seq™)
  • Poly(A) Enrichment
  • Ribosomal RNA Depletion
  • SARS-CoV-2 ARTIC sequencing

 

Which liquid-handling automation platforms have successfully automated NEBNext library prep kits?

Automation Icon
Automate DNA library prep
  Automation Icon
Automate RNA library prep

 

Automated library prep will save you time, but installing an automation script on your liquid handling robot can be labor intensive. Our in-house automation experts can help! Reach out and tell them about your project(s) today. 

 

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Automation for NEBNext® NGS Library Prep
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NEBNext Direct® Genotyping Solution

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NEBNext® Enzymatic Methyl-seq Conversion Module

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NEBNext® Enzymatic Methyl-seq Kit

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NEBNext® Multiplex Oligos for Illumina® (96 Index Primers)

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NEBNext® Ultra II Directional RNA Library Prep Kit for Illumina®

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NEBNext® Ultra II Directional RNA Library Prep with Sample Purification Beads

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NEBNext® Ultra™ II Directional RNA Second Strand Synthesis Module

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NEBNext® Ultra II DNA Library Prep with Sample Purification Beads

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NEBNext® Ultra II FS DNA Library Prep Kit for Illumina

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NEBNext® Ultra II FS DNA Library Prep with Sample Purification Beads

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NEBNext® Ultra II FS DNA Module

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NEBNext® Ultra™ II Q5® Master Mix

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NEBNext® Ultra II RNA First Strand Synthesis Module

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NEBNext® Ultra™ II FS DNA PCR-free Library Prep with Sample Purification Beads

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NEBNext® Ultra™ II DNA PCR-free Library Prep Kit for Illumina®

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NEBNext® Ultra™ II FS DNA PCR-free Library Prep Kit for Illumina®


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Application Notes for Automation for NEBNext® NGS Library Prep
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