Home PCR, qPCR & Amplification Technologies Taq DNA Polymerase with Standard Taq Buffer

Taq DNA Polymerase with Standard Taq Buffer

This product is available in a glycerol-free format. Contact us for more information.

Taq DNA Polymerase is the industry standard for PCR

Ideal for routine PCR applications
Exceptional value in terms of cost per unit
Hot start version available (NEB #M0495)

Catalog #	Cat #	Concentration	Conc	Size	List Price
M0273S	5,000 units/ml	400 units	$75.00	$75.00	Sign In Sign In
M0273L	5,000 units/ml	2,000 units	$295.00	$295.00	Sign In Sign In
M0273X	5,000 units/ml	4,000 units	$535.00	$535.00	Sign In Sign In
M0273E	5,000 units/ml	20,000 units	$1,426.00	$1,426.00	Sign In Sign In

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Product Information

[flamingo thumbnail] Are you using Taq? Try OneTaq® DNA Polymerase

Taq DNA Polymerase is a thermostable DNA polymerase that possesses a 5´→ 3´ polymerase activity (1,2,3) and a 5´ flap endonuclease activity (4,5).

It is supplied with 10X Standard Taq Reaction Buffer, which is detergent-free and designed to be compatible with existing assay systems.

Highlights

Isolated from a recombinant source
Supplied with 10X Reaction Buffer
Robust and reliable reactions
Tolerates a wide range of templates
Incorporates dUTP, dITP and fluorescently-labeled nucleotides

Product Source

An E. coli strain that carries the Taq DNA Polymerase gene from Thermus aquaticus YT-1

This product is related to the following categories:: Taq DNA Polymerase

This product can be used in the following applications:: Colony PCR,; A-tailing,; Polymerases for DNA Manipulation,; Multiplex PCR,; Specialty PCR,; Routine PCR,

Reagents Supplied

The following reagents are supplied with this product:

NEB # Component Name Component # Stored at (°C) Amount Concentration

M0273S

-20

	Taq DNA Polymerase with Standard Taq Buffer	M0273SVIAL	-20	1 x 0.08 ml	5,000 units/ml
	Standard Taq Reaction Buffer Pack	B9014SVIAL	-20	2 x 1.5 ml	10 X

M0273L

-20

	Taq DNA Polymerase with Standard Taq Buffer	M0273LVIAL	-20	1 x 0.4 ml	5,000 units/ml
	Standard Taq Reaction Buffer Pack	B9014SVIAL	-20	4 x 1.5 ml	10 X

M0273X

-20

	Taq DNA Polymerase with Standard Taq Buffer	M0273XVIAL	-20	1 x 0.8 ml	5,000 units/ml
	Standard Taq Reaction Buffer Pack	B9014SVIAL	-20	8 x 1.5 ml	10 X

M0273E

-20

	Taq DNA Polymerase with Standard Taq Buffer	M0273XVIAL	-20	5 x 0.8 ml	5,000 units/ml
	Standard Taq Reaction Buffer Pack	B9014SVIAL	-20	40 x 1.5 ml	10 X

Properties & Usage
Materials Required but not Supplied
- Template DNA and associated forward and reverse primers
- Deoxynucleotide (dNTP) Solution Mix (NEB #N0447)
- Nuclease-free Water (NEB #B1500)
Unit Definition
One unit is defined as the amount of enzyme that will incorporate 15 nmol of dNTP into acid-insoluble material in 30 minutes at 75°C.

Reaction Conditions

1X Standard Taq Reaction Buffer Pack

1X Standard Taq Reaction Buffer Pack
10 mM Tris-HCl
50 mM KCl
1.5 mM MgCl₂
(pH 8.3 @ 25°C)

Storage Buffer

10 mM Tris-HCl
100 mM KCl
1 mM DTT
0.1 mM EDTA
0.5% Tween® 20
0.5% IGEPAL® CA-630
50% Glycerol
pH 7.4 @ 25°C

Heat Inactivation
No
Molecular Weight
Theoretical: 94000 daltons

5' - 3' Exonuclease
Yes
3' - 5' Exonuclease
No
Strand Displacement
+
Resulting Ends
- Single-base 3´ Overhangs
Unit Assay Conditions
1X ThermoPol® Reaction Buffer, 200 µM dNTPs including [³H]-dTTP and 15 nM primed M13 DNA
Error Rate
~ 285x10^-6bases
Advantages and Features
Features

Application Features
- PCR
- Primer Extension
- DHPLC
- Microarray Analysis
- Colony PCR
Related Products
Companion Products
Materials Sold Separately
- Standard Taq Reaction Buffer Pack
Product Notes
1. 5'→3' flap endonuclease degrades displaced strand.
References
1. Chien, A., Edgar, D.B. and Trela, J.M. (1976). J. Bact.. 127, 1550-1557.
2. Kaledin, A.S., Sliusarenko, A.G. and Gorodetskii, S.I. (1980). Biokhimiya. 45, 644-651.
3. Lawyer, F.C. et al. (1993). PCRMethods and Appl.. 2, 275-287.
4. Longley, M.J., Bennett, S.E. and Mosbaugh D.W. (1990). Nucleic Acids Res.. 18, 7317-7322.
5. Lyamichev, V., Brow, M.A. and Dahlberg, J.E. (1993). Science. 260, 778-783.
6. Saiki R.K. et al. (1985). Science. 230, 1350-1354.
7. Powell, L.M. et al. (1987). Cell. 50, 831-840.
8. Sun, Y., Hegamyer, G. and Colburn, N. (1993). Biotechniques. 15, 372-374.
9. Sarkar, G., Kapelner, S. and Sommer, S.S. (1990). NucleicAcids Res.. 18, 7465.

Protocols, Manuals & Usage
- Protocols
  PCR Protocol for Taq DNA Polymerase with Standard Taq Buffer (NEB #M0273)
  
  A-Tailing with Taq Polymerase
- Usage Guidelines
  Activity of Restriction Enzymes in PCR Buffers
  
  Guidelines for PCR Optimization with Taq DNA Polymerase
  
  Guidelines for PCR Optimization with Thermophilic DNA Polymerases
Tools & Resources
- Selection Charts
  DNA Polymerase Selection Chart
  
  Taq Buffer Selection Chart
  
  Thermophilic DNA Polymerases
- Web Tools
  PCR Selector
  
  Tm Calculator
FAQs & Troubleshooting
- FAQs
  What ends will my PCR products have?
  
  How should I determine the appropriate annealing temperature for my reaction?
  
  What should the final primer concentration be in my PCR?
  
  What are the properties of this polymerase (fidelity, product ends, max amplicon, modified base incorporation, etc.)?
  
  What are the stability and storage requirements for NEB's Taq and OneTaq DNA Polymerases?
  
  Is this Taq DNA Polymerase product compatible with other NEB Buffers?
  
  Can Taq/OneTaq® DNA Polymerases be used for nick translation?
  
  How can I optimize my PCR when using Taq DNA Polymerase?
- Troubleshooting
  PCR Troubleshooting Guide
  
  Taq PCR Kit Troubleshooting Guide
- Tech Tips
  
  Did you know most Taq reactions amplify more efficiently and robustly when you use a 68°C extension temperature?
Citations & Technical Literature
- Citations
  
  Product Citation Tool
  
  Powered by Bioz See more details on Bioz
Quality, Safety & Legal
- Quality Control Assays
  
  Quality Control tests are performed on each new lot of NEB product to meet the specifications designated for it. Specifications and individual lot data from the tests that are performed for this particular product can be found and downloaded on the Product Specification Sheet, Certificate of Analysis, data card or product manual. Further information regarding NEB product quality can be found here.
- Specifications & Change Notifications
  The Specification sheet is a document that includes the storage temperature, shelf life and the specifications designated for the product. The following file naming structure is used to name these document files: [Product Number]_[Size]_[Version]
  
  M0273S_L_X_E_v2
  
  M0273S_L_X_E_G_v3
- Certificate of Analysis
  The Certificate of Analysis (COA) is a signed document that includes the storage temperature, expiration date and quality controls for an individual lot. The following file naming structure is used to name these document files: [Product Number]_[Size]_[Version]_[Lot Number]
  
  M0273S_L_X_E_v1_0141510
  
  M0273S_L_X_E_v1_0141512
  
  M0273S_L_X_E_v1_0141509
  
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- Safety Data Sheets
  The following is a list of Safety Data Sheet (SDS) that apply to this product to help you use it safely.
  
  Taq DNA Polymerase with Standard Taq Buffer
  
  Standard Taq Reaction Buffer Pack
- Legal and Disclaimers
  
  Products and content are covered by one or more patents, trademarks and/or copyrights owned or controlled by New England Biolabs, Inc (NEB). The use of trademark symbols does not necessarily indicate that the name is trademarked in the country where it is being read; it indicates where the content was originally developed. The use of this product may require the buyer to obtain additional third-party intellectual property rights for certain applications. For more information, please email busdev@neb.com.
  
  This product is intended for research purposes only. This product is not intended to be used for therapeutic or diagnostic purposes in humans or animals.
  
  New England Biolabs (NEB) is committed to practicing ethical science – we believe it is our job as researchers to ask the important questions that when answered will help preserve our quality of life and the world that we live in. However, this research should always be done in safe and ethical manner. Learn more.
  Trademarks
  NEW ENGLAND BIOLABS®, QUICK-LOAD® and THERMOPOL® are registered trademarks of New England Biolabs, Inc.
  
  IGEPAL^®is a registered trademark of Rhodia Operations.
  TWEEN^®is a registered trademark of Uniqema Americas, LLC.

Taq DNA Polymerase with Standard Taq Buffer

Featured Video

Product Information

Highlights

Product Source

Reagents Supplied

Properties & Usage

Materials Required but not Supplied

Unit Definition

Reaction Conditions

Storage Buffer

Heat Inactivation

Molecular Weight

5' - 3' Exonuclease

3' - 5' Exonuclease

Strand Displacement

Resulting Ends

Unit Assay Conditions

Error Rate

Advantages and Features

Features

Application Features

Related Products

Companion Products

Materials Sold Separately

Product Notes

References

Protocols, Manuals & Usage

Protocols

Usage Guidelines

Tools & Resources

Selection Charts

Web Tools

FAQs & Troubleshooting

FAQs

Troubleshooting

Tech Tips

Citations & Technical Literature

Citations

Product Citation Tool

Quality, Safety & Legal

Quality Control Assays

Specifications & Change Notifications

Certificate of Analysis

Safety Data Sheets

Legal and Disclaimers

Trademarks

Featured Videos

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