T4 RNA Ligase 2 (dsRNA Ligase)

The following reagents are supplied with this product:

NEB #	Component Name	Component #	Stored at (°C)	Amount	Concentration
M0239S     -20       T4 RNA Ligase 2 (dsRNA Ligase) M0239SVIAL -20 1 x 0.015 ml 10,000 units/ml   T4 Rnl2 Reaction Buffer B0239SVIAL -20 1 x 1.5 ml 10 X
M0239L     -20       T4 RNA Ligase 2 (dsRNA Ligase) M0239LVIAL -20 1 x 0.075 ml 10,000 units/ml   T4 Rnl2 Reaction Buffer B0239SVIAL -20 1 x 1.5 ml 10 X

Application Features

• Ligate a nick in dsRNA
• Ligate the 3´ OH of RNA to the 5´ phosphate of DNA in a double stranded structure

Companion Products

• T4 RNA Ligase 1 (ssRNA Ligase)
• RNase Inhibitor, Murine

1. Ho, C.K. and Shuman, S. (2002). Proc. Natl. Acad. Sci. USA. 99, 12709-12714.
2. Ho, C.K. et al. (2004). Structure. 12, 327-339.
3. Nandakumar, J. et al. (2004). J. Biol. Chem.. 279, 31337-31347.
4. Nandakuman, J. and Shuman, S. (2004). Mol. Cell. 16, 211-221.

1. Protocol for ligation of Nicks in dsRNA with T4 RNA Ligase 2 (NEB #M0239)
2. Protocol for ligation of 3′OH of RNA and 5′PO4 of DNA in ds structure with T4 RNA Ligase 2 (NEB #M0239)

• Properties of DNA and RNA Ligases
• RNA Ligase Selection Chart
• Substrate-based Ligase Selection Chart

1. How can I achieve optimal ligation of a 5’ phosphorylated ssDNA oligo to the 3’ end of an ssRNA substrate with a ssDNA or ssRNA splint using T4 RNA Ligase 2?
2. What is the salt tolerance of this RNA ligase?

Quality Control tests are performed on each new lot of NEB product to meet the specifications designated for it. Specifications and individual lot data from the tests that are performed for this particular product can be found and downloaded on the Product Specification Sheet, Certificate of Analysis, data card or product manual. Further information regarding NEB product quality can be found here.

The Specification sheet is a document that includes the storage temperature, shelf life and the specifications designated for the product. The following file naming structure is used to name these document files: [Product Number]_[Size]_[Version]

• M0239S_L_v2

The Certificate of Analysis (COA) is a signed document that includes the storage temperature, expiration date and quality controls for an individual lot. The following file naming structure is used to name these document files: [Product Number]_[Size]_[Version]_[Lot Number]

• M0239L_v1_10016831
• M0239L_v1_10029433
• M0239L_v1_10031202
• M0239L_v1_10039469
• M0239S_v1_10039471
• M0239L_v1_10056214
• M0239S_v1_10052770
• M0239L_v1_10059007
• M0239S_v1_10056216
• M0239L_v1_10062561
• M0239L_v1_10068405
• M0239L_v1_10077151
• M0239S_v1_10081143
• M0239L_v1_10080149
• M0239L_v1_10088324
• M0239S_v1_10088326
• M0239L_v1_10102475
• M0239L_v1_10102473
• M0239S_v1_10120087
• M0239L_v1_10124275
• M0239S_v1_10146850
• M0239S_v1_10156049
• M0239L_v1_10158106
• M0239S_v1_10167889
• M0239L_v1_10176627
• M0239L_v1_10178719
• M0239L_v1_10181512
• M0239S_v1_10183839
• M0239L_v1_10200635
• M0239S_L_v1_0041802
• M0239S_v1_10220914
• M0239L_v1_10226275
• M0239S_v1_10246121
• M0239S_v1_10272939
• M0239L_v1_10272940
• M0239L_v1_10294726
• M0239S_v1_10305933
• M0239L_v1_10313287
• M0239S_v2_10328453

The following is a list of Safety Data Sheet (SDS) that apply to this product to help you use it safely.

• T4 RNA Ligase 2 (dsRNA Ligase)
• T4 Rnl2 Reaction Buffer

Products and content are covered by one or more patents, trademarks and/or copyrights owned or controlled by New England Biolabs, Inc (NEB). The use of trademark symbols does not necessarily indicate that the name is trademarked in the country where it is being read; it indicates where the content was originally developed. The use of this product may require the buyer to obtain additional third-party intellectual property rights for certain applications. For more information, please email busdev@neb.com.

This product is intended for research purposes only. This product is not intended to be used for therapeutic or diagnostic purposes in humans or animals.

New England Biolabs (NEB) is committed to practicing ethical science – we believe it is our job as researchers to ask the important questions that when answered will help preserve our quality of life and the world that we live in. However, this research should always be done in safe and ethical manner. Learn more.