DNA Polymerase I (E. coli)

The following reagents are supplied with this product:

NEB #	Component Name	Component #	Stored at (°C)	Amount	Concentration
M0209S     -20       DNA Polymerase I (E. coli) M0209SVIAL -20 1 x 0.05 ml 10,000 units/ml   NEBuffer™ 2 B7002SVIAL -20 1 x 1.25 ml 10 X
M0209L     -20       DNA Polymerase I (E. coli) M0209LVIAL -20 1 x 0.25 ml 10,000 units/ml   NEBuffer™ 2 B7002SVIAL -20 1 x 1.25 ml 10 X

Application Features

• Nick translation of DNA to obtain probes with a high specific activity (2)
• Second strand synthesis of cDNA (3,4)

Companion Products

• Deoxynucleotide (dNTP) Solution Mix
• Deoxynucleotide (dNTP) Solution Set
• DNase I (RNase-free)

Materials Sold Separately

• NEBuffer 2

1. DNase I is not included with this enzyme and must be added for nick translation reactions.
2. DNA Polymerase I (E.coli) is active in all four NEBuffers when supplemented with dNTPs (not included).

1. Lehman, I.R. (1981). In P.D. Boyer(Ed.), The Enzymes. 14A, 16-38. San Diego: Academic Press.
2. Meinkoth, J. and Wahl, G.M (1987). S.L. Berger and A.R. Kimmel(Ed.), Methods Enzymol.. 152, 91-94. San Diego: Academic Press.
3. Gubler, U. and Hoffmann, B.J. (1983). Gene. 25, 263-269.
4. D'Alessio, J.M. and Gerard, G.F. (1988). Nucl. Acids Res.. 16, 1999-2014.
5. Kunkel, T.A., Loeb, L.A. and Goodman, M.F. (1984). J. Biol. Chem. . 259, 1539-1545.

• Activity of DNA Modifying Enzymes in rCutSmart™ Buffer

• DNA Polymerase Selection Chart

1. Which DNA polymerase can I use to blunt DNA?
2. Can I use a DNA polymerase to fill in 3' overhangs or remove 5' overhangs?
3. How can I heat inactivate this DNA polymerase?
4. Can this DNA polymerase be used in labeling and partial fill reactions?
5. Can this DNA polymerase be used in other NEBuffers for filling in or blunting overhangs?

Quality Control tests are performed on each new lot of NEB product to meet the specifications designated for it. Specifications and individual lot data from the tests that are performed for this particular product can be found and downloaded on the Product Specification Sheet, Certificate of Analysis, data card or product manual. Further information regarding NEB product quality can be found here.

The Specification sheet is a document that includes the storage temperature, shelf life and the specifications designated for the product. The following file naming structure is used to name these document files: [Product Number]_[Size]_[Version]

• M0209S_L_v2

The Certificate of Analysis (COA) is a signed document that includes the storage temperature, expiration date and quality controls for an individual lot. The following file naming structure is used to name these document files: [Product Number]_[Size]_[Version]_[Lot Number]

• M0209S_L_v1_0921509
• M0209S_L_v1_0921603
• M0209S_L_v1_0921608
• M0209S_L_v1_0991703
• M0209S_L_v1_0991709
• M0209S_L_v1_0991803
• M0209L_v1_10020363
• M0209S_v1_10019923
• M0209L_v1_10045699
• M0209S_v1_10046610
• M0209L_v1_10051040
• M0209L_v1_10055153
• M0209L_v1_10060130
• M0209S_v1_10060132
• M0209L_v1_10063897
• M0209L_v1_10069135
• M0209L_v1_10071622
• M0209S_v1_10077358
• M0209S_v1_10090580
• M0209L_v1_10088385
• M0209L_v1_10099483
• M0209S_v1_10104375
• M0209L_v1_10109893
• M0209S_v1_10126661
• M0209L_v1_10133274
• M0209L_v1_10147215
• M0209S_v1_10152884
• M0209S_v1_10167678
• M0209L_v1_10178505
• M0209L_v1_10184594
• M0209S_v1_10191588
• M0209L_v1_10200478
• M0209S_v1_10210681
• M0209L_v1_10221145
• M0209S_v1_10221144
• M0209L_v1_10210695
• M0209L_v1_10234115
• M0209L_v1_10243113
• M0209S_v1_10246034
• M0209L_v2_10261181
• M0209L_v2_10269489
• M0209S_v2_10269488
• M0209L_v2_10281469
• M0209S_v2_10281965
• M0209L_v2_10286994
• M0209L_v2_10299299
• M0209S_v2_10308898
• M0209L_v2_10309154
• M0209L_v2_10321855
• M0209S_v2_10323055
• M0209L_v2_10331640
• M0209S_v2_10336826

The following is a list of Safety Data Sheet (SDS) that apply to this product to help you use it safely.

• DNA Polymerase I (E. coli)
• NEBuffer™ 2

Products and content are covered by one or more patents, trademarks and/or copyrights owned or controlled by New England Biolabs, Inc (NEB). The use of trademark symbols does not necessarily indicate that the name is trademarked in the country where it is being read; it indicates where the content was originally developed. The use of this product may require the buyer to obtain additional third-party intellectual property rights for certain applications. For more information, please email busdev@neb.com.

This product is intended for research purposes only. This product is not intended to be used for therapeutic or diagnostic purposes in humans or animals.

New England Biolabs (NEB) is committed to practicing ethical science – we believe it is our job as researchers to ask the important questions that when answered will help preserve our quality of life and the world that we live in. However, this research should always be done in safe and ethical manner. Learn more.