Taq 5X Master Mix

The following reagents are supplied with this product:

NEB #	Component Name	Component #	Stored at (°C)	Amount	Concentration
M0285L     -20       Taq 5X Master Mix M0285SVIAL -20 5 x 1 ml 5 X   Magnesium Chloride (MgCl2) Solution B9021SVIAL -20 3 x 1.5 ml 25 mM

Application Features

• PCR
• Primer Extension
• Colony PCR

Companion Products

• Taq DNA Polymerase with Standard Taq Buffer
• Deoxynucleotide (dNTP) Solution Set
• Deoxynucleotide (dNTP) Solution Mix
• EpiMark® Methylated DNA Enrichment Kit

Materials Sold Separately

• Magnesium Chloride (MgCl₂) Solution

1. Taq 5X Master Mix is stable for fifteen freeze-thaw cycles when stored at -20°C
2. Taq 5X Master Mix is also stable for six months at 4°C, so for frequent use, an aliquot may be kept at 4°C.
3. Taq 5X Master Mix should be used at a 1X concentration with DNA template and primers in a total reaction volume of 25 or 50 μl.

1. Chien, A., Edgar, D.B. and Trela, J.M. (1976). J. Bact.. 127, 1550-1557.
2. Kaledin, A.S., Sliusarenko, A.G. and Gorodetskii, S.I. (1980). Biokhimiya. 45, 644-651.
3. Lawyer, F.C. et al. (1993). PCR Methods and Appl.. 2, 275-287.
4. Longley, M.J., Bennett, S.E. and Mosbaugh D.W. (1990). Nucleic Acids Res.. 18, 7317-7322.
5. Lyamichev, V., Brow, M.A. and Dahlberg, J.E. (1993). Science. 260, 778-783.
6. Saiki R.K. et al. (1985). Science. 230, 1350-1354.
7. Powell, L.M. et al. (1987). Cell. 50, 831-840.
8. Sun, Y., Hegamyer, G. and Colburn, N. (1993). Biotechniques. 15, 372-374.
9. Sarkar, G., Kapelner, S. and Sommer, S.S. (1990). Nucleic Acids Res.. 18, 7465.

1. Protocol for Taq 5X Master Mix (M0285)

• Activity of Restriction Enzymes in PCR Buffers
• Guidelines for PCR Optimization with Taq DNA Polymerase
• Guidelines for PCR Optimization with Thermophilic DNA Polymerases

• DNA Polymerase Selection Chart
• Thermophilic DNA Polymerases

• PCR Selector
• Tm Calculator

1. What ends will my PCR products have?
2. How should I determine the appropriate annealing temperature for my reaction?
3. What should the final primer concentration be in my PCR?
4. What are the properties of this polymerase (fidelity, product ends, max amplicon, modified base incorporation, etc.)?
5. What are the stability and storage requirements for NEB's Taq and OneTaq DNA Polymerases?
6. Can Taq/OneTaq® DNA Polymerases be used for nick translation?
7. How can I optimize my PCR when using Taq DNA Polymerase?

• PCR Troubleshooting Guide
• Taq PCR Kit Troubleshooting Guide

Quality Control tests are performed on each new lot of NEB product to meet the specifications designated for it. Specifications and individual lot data from the tests that are performed for this particular product can be found and downloaded on the Product Specification Sheet, Certificate of Analysis, data card or product manual. Further information regarding NEB product quality can be found here.

The Specification sheet is a document that includes the storage temperature, shelf life and the specifications designated for the product. The following file naming structure is used to name these document files: [Product Number]_[Size]_[Version]

• M0285S_L_v3

The Certificate of Analysis (COA) is a signed document that includes the storage temperature, expiration date and quality controls for an individual lot. The following file naming structure is used to name these document files: [Product Number]_[Size]_[Version]_[Lot Number]

• M0285S_L_v1_0201702
• M0285S_L_v1_0211708
• M0285S_L_v1_0221803
• M0285L_v1_10014390
• M0285L_v1_10022736
• M0285L_v1_10026025
• M0285L_v1_10029425
• M0285L_v1_10031125
• M0285L_v1_10032689
• M0285L_v1_10033856
• M0285L_v1_10037190
• M0285L_v1_10042502
• M0285L_v1_10047018
• M0285L_v1_10049769
• M0285L_v1_10056700
• M0285L_v1_10060134
• M0285L_v2_10069613
• M0285L_v2_10073569
• M0285L_v2_10079651
• M0285L_v2_10082436
• M0285L_v2_10086684
• M0285L_v2_10088165
• M0285L_v2_10098095
• M0285L_v2_10104382
• M0285L_v2_10107647
• M0285L_v2_10119086
• M0285L_v2_10127912
• M0285L_v2_10135763
• M0285L_v2_10140995
• M0285L_v2_10146685
• M0285L_v2_10157009
• M0285L_v2_10160667
• M0285L_v2_10162795
• M0285L_v2_10167008
• M0285L_v2_10170227
• M0285L_v2_10174183
• M0285L_v2_10181980
• M0285L_v2_10191597
• M0285L_v2_10203811
• M0285L_v2_10209407
• M0285L_v2_10219576
• M0285L_v2_10233892
• M0285L_v3_10250299
• M0285L_v3_10263632
• M0285L_v3_10276413
• M0285L_v3_10282653
• M0285L_v3_10289026
• M0285L_v3_10298989
• M0285L_v3_10304233
• M0285L_v3_10325236

The following is a list of Safety Data Sheet (SDS) that apply to this product to help you use it safely.

• Taq 5X Master Mix
• Magnesium Chloride (MgCl₂) Solution

Products and content are covered by one or more patents, trademarks and/or copyrights owned or controlled by New England Biolabs, Inc (NEB). The use of trademark symbols does not necessarily indicate that the name is trademarked in the country where it is being read; it indicates where the content was originally developed. The use of this product may require the buyer to obtain additional third-party intellectual property rights for certain applications. For more information, please email busdev@neb.com.

This product is intended for research purposes only. This product is not intended to be used for therapeutic or diagnostic purposes in humans or animals.

New England Biolabs (NEB) is committed to practicing ethical science – we believe it is our job as researchers to ask the important questions that when answered will help preserve our quality of life and the world that we live in. However, this research should always be done in safe and ethical manner. Learn more.

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