phi29 DNA Polymerase

The following reagents are supplied with this product:

NEB #	Component Name	Component #	Stored at (°C)	Amount	Concentration
M0269S     -20       phi29 DNA Polymerase M0269SVIAL -20 1 x 0.025 ml 10,000 units/ml   phi29 DNA Polymerase Reaction Buffer B0269SVIAL -20 1 x 1.5 ml 10 X   Recombinant Albumin, Molecular Biology Grade B9200SVIAL -20 1 x 0.6 ml 20 mg/ml
M0269L     -20       phi29 DNA Polymerase M0269LVIAL -20 1 x 0.125 ml 10,000 units/ml   phi29 DNA Polymerase Reaction Buffer B0269SVIAL -20 1 x 1.5 ml 10 X   Recombinant Albumin, Molecular Biology Grade B9200SVIAL -20 1 x 0.6 ml 20 mg/ml

Application Features

• Replication requiring a high degree of strand displacement and/or processive synthesis
• High fidelity replication at moderate temperatures

Companion Products

• Deoxynucleotide (dNTP) Solution Mix
• Deoxynucleotide (dNTP) Solution Set

Materials Sold Separately

• Recombinant Albumin, Molecular Biology Grade
  
  
  
  
  

1. The presence of active reducing reagent in the reaction buffer is critical for this enzyme. While the reaction buffer supplied with the enzyme contains DTT, older buffer stocks or stocks that have been repeatedly frozen and thawed should be supplemented with 4 mM DTT to obtain maximal activity.

1. Blanco, L. and Salas, M. (1984). Proc. Natl. Acad. Sci. USA. 81, 5325-5329.
2. Blanco. L., et al. (1989). J. Biol. Chem.. 264, 8935-8940.
3. Garmendia, C., et al. (1992). J. Biol. Chem.. 267, 2594-2599.

• Activity of DNA Modifying Enzymes in rCutSmart™ Buffer

• DNA Polymerase Selection Chart

1. Can phi29 DNA Polymerase be used in other NEBuffers?
2. Can phi29 DNA Polymerase be used to blunt DNA?
3. Can phi29 DNA Polymerase be used to fill in 3' overhangs?
4. Can phi29 DNA Polymerase be used to remove 5' overhangs?
5. Can phi29 DNA Polymerase be heat inactivated?
6. At what rate does phi29 DNA Polymerase add nucleotides to a primed single-stranded template?
7. Will phi29 DNA Polymerase work in rCutSmart buffer?
8. Are NEB DNA Polymerases supplied with dNTPs?

Quality Control tests are performed on each new lot of NEB product to meet the specifications designated for it. Specifications and individual lot data from the tests that are performed for this particular product can be found and downloaded on the Product Specification Sheet, Certificate of Analysis, data card or product manual. Further information regarding NEB product quality can be found here.

The Specification sheet is a document that includes the storage temperature, shelf life and the specifications designated for the product. The following file naming structure is used to name these document files: [Product Number]_[Size]_[Version]

• M0269S_L_v3

The Certificate of Analysis (COA) is a signed document that includes the storage temperature, expiration date and quality controls for an individual lot. The following file naming structure is used to name these document files: [Product Number]_[Size]_[Version]_[Lot Number]

• M0269S_L_v1_0131412
• M0269S_L_v1_0131506
• M0269S_L_v1_0131512
• M0269S_L_v2_0131606
• M0269S_L_v2_0131612
• M0269S_L_v2_0131712
• M0269L_v2_10014232
• M0269S_v2_10013602
• M0269L_v2_10028010
• M0269L_v2_10032150
• M0269S_v2_10034467
• M0269L_v2_10034472
• M0269L_v2_10042808
• M0269L_v2_10047143
• M0269L_v2_10049721
• M0269S_v2_10051885
• M0269S_v3_10055099
• M0269L_v3_10055100
• M0269S_v3_10058026
• M0269L_v3_10061055
• M0269S_v3_10062498
• M0269S_v3_10065462
• M0269L_v3_10065461
• M0269L_v3_10079525
• M0269L_v3_10082437
• M0269L_v3_10088096
• M0269S_v3_10088150
• M0269L_v3_10091533
• M0269S_v3_10097974
• M0269L_v3_10097975
• M0269L_v3_10106254
• M0269S_v3_10113084
• M0269L_v3_10112790
• M0269L_v3_10117815
• M0269L_v3_10122831
• M0269L_v3_10127410
• M0269S_v3_10123955
• M0269L_v3_10130842
• M0269S_v3_10116357
• M0269L_v3_10138426
• M0269L_v3_10146118
• M0269L_v3_10155129
• M0269S_v3_10153242
• M0269L_v3_10158332
• M0269L_v3_10160065
• M0269L_v3_10161332
• M0269S_v3_10161094
• M0269L_v3_10164966
• M0269S_v3_10164967
• M0269S_v3_10172270
• M0269L_v3_10171024
• M0269L_v3_10178663
• M0269S_v3_10178671
• M0269L_v3_10185360
• M0269L_v3_10191592
• M0269L_v3_10201722
• M0269S_v3_10203092
• M0269L_v3_10206023
• M0269L_v3_10208016
• M0269S_v3_10206021
• M0269S_v3_10210722
• M0269L_v3_10218706
• M0269S_v3_10223539
• M0269L_v3_10233854
• M0269S_v3_10238464
• M0269L_v3_10243053
• M0269S_v3_10250266
• M0269L_v3_10253468
• M0269S_v3_10260344
• M0269L_v3_10267547
• M0269S_v3_10267549
• M0269L_v3_10274501
• M0269L_v3_10280869
• M0269S_v3_10285223
• M0269S_v3_10287685
• M0269L_v3_10287711
• M0269L_v3_10291536
• M0269L_v3_10300198
• M0269S_v3_10298243
• M0269S_v3_10302928
• M0269L_v3_10304236
• M0269S_v3_10311351
• M0269S_v3_10321534
• M0269L_v3_10330853
• M0269S_v3_10335934

The following is a list of Safety Data Sheet (SDS) that apply to this product to help you use it safely.

• phi29 DNA Polymerase
• phi29 DNA Polymerase Reaction Buffer
• Recombinant Albumin, Molecular Biology Grade

Products and content are covered by one or more patents, trademarks and/or copyrights owned or controlled by New England Biolabs, Inc (NEB). The use of trademark symbols does not necessarily indicate that the name is trademarked in the country where it is being read; it indicates where the content was originally developed. The use of this product may require the buyer to obtain additional third-party intellectual property rights for certain applications. For more information, please email busdev@neb.com.

This product is intended for research purposes only. This product is not intended to be used for therapeutic or diagnostic purposes in humans or animals.

New England Biolabs (NEB) is committed to practicing ethical science – we believe it is our job as researchers to ask the important questions that when answered will help preserve our quality of life and the world that we live in. However, this research should always be done in safe and ethical manner. Learn more.

Licenses

Use of this material in DNA sequencing and/or DNA amplification may require a license from GE Healthcare. Any persons interested in using this product in such applications are advised to contact GE Healthcare, 800 Centennial Avenue, P.O. Box 1327, Piscataway, NJ 08855–1327, Attention: Patent Department.

Trademarks

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