α1-2 Fucosidase is a highly specific exoglycosidase that catalyzes the hydrolysis of linear α1-2 linked fucose residues from oligosaccharides
Recombinant enzyme with no detectable endoglycosidase or other exoglycosidase contaminating activities
Glycerol-free for optimal performance in HPLC and mass spectrometry analysis
≥95% purity, as determined by SDS-PAGE and intact ESI-MS
Optimal activity and stability for up to 24 months
Product Information
Substrate Specificity:α1-2 Fucosidase is a highly specific exoglycosidase that catalyzes the hydrolysis of linear α1-2 linked L-fucopyranosyl residues from oligosaccharides (1). In this case, a linear substrate is defined as having no branching on the adjacent residue.
Figure 1: Detailed Specificity of α1-2 Fucosidase All reactions contained 1X Glycobuffer 1. All reactions contain 1X rHSA in a total volume of 10 μl, and all reactions were incubated at 37°C. All reactions contained 20 units of α1-2 fucosidase. Reaction (C) also contained 20 units of α-N-Acetylgalactosaminidase (#P0734). In Reaction (C), the branched α 1-2 fucose is removed in the presence of both enzymes, but not by α1-2 Fucosidase alone.
Product Source
Cloned from Xanthomonas manihotis and expressed in E. coli.
This product is related to the following categories:
The following reagents are supplied with this product:
NEB #
Component Name
Component #
Stored at (°C)
Amount
Concentration
P0724S
4
α1-2 Fucosidase
P0724SVIAL
4
1 x 0.05 ml
20,000 units/ml
GlycoBuffer 1
B1727SVIAL
-20
1 x 1 ml
10 X
Recombinant Albumin (Low-glycerol)
B9203SVIAL
-20
1 x 0.25 ml
10 mg/ml
P0724L
4
α1-2 Fucosidase
P0724LVIAL
4
1 x 0.25 ml
20,000 units/ml
GlycoBuffer 1
B1727SVIAL
-20
1 x 1 ml
10 X
Purified BSA
B9001SVIAL
-20
1 x 0.25 ml
10 mg/ml
Properties & Usage
Unit Definition
One unit is defined as the amount of enzyme required to cleave > 95% of the α-L-fucose from 1 nmol of Fucα1-2Galβ1-4Glc-7-amino-4-methyl-coumarin (AMC), in 1 hour at 37°C in a total reaction volume of 10 µl.
Reaction Conditions
1X GlycoBuffer 1
Supplement with 100 µg/ml Recombinant Albumin (Low-glycerol)
Incubate at 37°C
1X GlycoBuffer 1
5 mM CaCl2
50 mM sodium acetate
(pH 5.5 @ 25°C)
Storage Buffer
50 mM NaCl
20 mM Tris-HCl
1 mM EDTA
pH 7.5 @ 25°C
Heat Inactivation
65°C for 10 minutes
Molecular Weight
Theoretical: 70 kDa
Unit Assay Conditions
Two fold dilutions of α1-2 Fucosidase are incubated with 1 nmol AMC-labeled substrate in 1X GlycoBuffer 1, supplemented with 100 µg/ml rHSA, in a 10 µl reaction. The reaction mix is incubated for 1 hour at 37°C. Separation of reaction products are visualized via thin layer chromatography (1).
Quality Control tests are performed on each new lot of NEB product to meet the specifications designated for it. Specifications and individual lot data from the tests that are performed for this particular product can be found and downloaded on the Product Specification Sheet, Certificate of Analysis, data card or product manual. Further information regarding NEB product quality can be found here.
Specifications & Change Notifications
Effective April 4, 2026, the shelf life has been extended from 1 year to 2 years at 4°C.
The Specification sheet is a document that includes the storage temperature, shelf life and the specifications designated for the product. The following file naming structure is used to name these document files: [Product Number]_[Size]_[Version]
The Certificate of Analysis (COA) is a signed document that includes the storage temperature, expiration date and quality controls for an individual lot. The following file naming structure is used to name these document files: [Product Number]_[Size]_[Version]_[Lot Number]
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This product is intended for research purposes only. This product is not intended to be used for therapeutic or diagnostic purposes in humans or animals.
New England Biolabs (NEB) is committed to practicing ethical science – we believe it is our job as researchers to ask the important questions that when answered will help preserve our quality of life and the world that we live in. However, this research should always be done in safe and ethical manner. Learn more.
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