Home Glycobiology Endo-β-Galactosidase

Endo-β-Galactosidase
Gray property icon for NEBU Gray property icon for cloned at NEB recombinant Gray property icon for incubation temperature 37° 55 Heat

  • Broader substrate specificity and higher activity compared to other enzymes from Escherichia freundii and Bacteroides fragilis
  • Recombinant enzyme with no detectable endoglycosidase or other exoglycosidase contaminating activities
  • Glycerol-free for optimal performance in HPLC and mass spectrometry analysis
  • ≥95% purity, as determined by SDS-PAGE and intact ESI-MS
 
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Catalog # Cat # Concentration Conc Size List Price List Price Your Price Quantity
P0777S 0.5 mg/ml 50 µg $403.00 $403.00
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  • Endo-β-Galactosidase is an endoglycosidase that hydrolyzes internal β(1-4)-galactose linkages within a variety of sulfated and nonsulfated poly-N-acetyllactosamine structures, [Galβ(1-4)-↓-GlcNAcβ(1-3)], derived from milk, glycoproteins and glycolipids. This enzyme is used for structural and functional analysis of poly-N-acetyllactosamine in glycoconjugates.

    This endo-beta-galactosidase (endo-b-galactosidase) enzyme from F. keratolyticus exhibits broader substrate specificity and higher catalytic efficiency compared to others from E. freundii and B. fragilis.

    Specificity:

    Mechanism of Endo-B-galactosidase

    null

    Product Source

    Cloned from Flavobacterium keratolyticus and expressed in E. coli
    This product is related to the following categories:
    Endoglycosidases
    This product can be used in the following applications:
    Glycoprotein Analysis

    • Reagents Supplied

      The following reagents are supplied with this product:

      NEB # Component Name Component # Stored at (°C) Amount Concentration
      • P0777S     -20    
          Endo-β-Galactosidase P0777SVIAL -20 1 x 50 µg 0.5 mg/ml
          GlycoBuffer 3 B1720SVIAL -20 1 x 1 ml 10 X

    • Properties & Usage

      Unit Definition

      The enzyme activity is determined by its ability to cleave over 95% of 2 nmol Galβ1-4GlcNAcβ1-3Galβ1-4GlcNAcβ1-3Galβ1-4Glc-7-amino-4-methyl-coumarin (AMC) using less than 10 ng of enzyme within 1 hour at 37°C, in a total reaction volume of 20 μl.

      Reaction Conditions

      1X GlycoBuffer 3
      Incubate at 37°C

      1X GlycoBuffer 3
      50 mM sodium acetate
      (pH 6 @ 25°C)

      Storage Buffer

      50 mM sodium acetate
      50 mM NaCl
      pH 5.5 @ 25°C

      Heat Inactivation

      55°C for 10 minutes

      Molecular Weight

      Apparent: 40.8 kDa

      Unit Assay Conditions

      Two fold dilutions of Endo-β-Galactosidase are incubated with 2 nmol AMC labeled substrate in 1X GlycoBuffer 3 in a 20 μl reaction. The reaction mix is incubated at 37°C for 1 hour. Separation of reaction products are visualized via thin layer chromatography.

    • Advantages and Features

      Features

      • This endo-beta-galactosidase (endo-b-galactosidase) enzyme from F. keratolyticus exhibits broader substrate specificity and higher catalytic efficiency compared to others from E. freundii and B. fragilis.
      • The enzyme is active in a wide range of pH conditions (pH 3.5 to pH 9.0).
      • The enzyme works at 37°C and below.

      This enzyme is ideal for:

      • Removal of poly-N-acetyllactosamine in glycoproteins and glycolipids.
      • Structural and functional analysis of poly-N-acetyllactosamine in glycoconjugates.

    • Related Products

      Companion Products

  • Protocols, Manuals & Usage

  • Quality, Safety & Legal

    • Quality Control Assays

      Quality Control tests are performed on each new lot of NEB product to meet the specifications designated for it. Specifications and individual lot data from the tests that are performed for this particular product can be found and downloaded on the Product Specification Sheet, Certificate of Analysis, data card or product manual. Further information regarding NEB product quality can be found here.

    • Specifications & Change Notifications

      The Specification sheet is a document that includes the storage temperature, shelf life and the specifications designated for the product. The following file naming structure is used to name these document files: [Product Number]_[Size]_[Version]

    • Certificate of Analysis

      The Certificate of Analysis (COA) is a signed document that includes the storage temperature, expiration date and quality controls for an individual lot. The following file naming structure is used to name these document files: [Product Number]_[Size]_[Version]_[Lot Number]

    • Safety Data Sheets

      The following is a list of Safety Data Sheet (SDS) that apply to this product to help you use it safely.

    • Legal and Disclaimers

      Products and content are covered by one or more patents, trademarks and/or copyrights owned or controlled by New England Biolabs, Inc (NEB). The use of trademark symbols does not necessarily indicate that the name is trademarked in the country where it is being read; it indicates where the content was originally developed. The use of this product may require the buyer to obtain additional third-party intellectual property rights for certain applications. For more information, please email busdev@neb.com.

      This product is intended for research purposes only. This product is not intended to be used for therapeutic or diagnostic purposes in humans or animals.

      New England Biolabs (NEB) is committed to practicing ethical science – we believe it is our job as researchers to ask the important questions that when answered will help preserve our quality of life and the world that we live in. However, this research should always be done in safe and ethical manner. Learn more.

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