M-MuLV Reverse Transcriptase

The following reagents are supplied with this product:

NEB #	Component Name	Component #	Stored at (°C)	Amount	Concentration
M0253S     -20       M-MuLV Reverse Transcriptase M0253SVIAL -20 1 x 0.05 ml 200,000 units/ml   M-MuLV Reverse Transcriptase Reaction Buffer B0253SVIAL -20 1 x 1.5 ml 10 X
M0253L     -20       M-MuLV Reverse Transcriptase M0253LVIAL -20 1 x 0.25 ml 200,000 units/ml   M-MuLV Reverse Transcriptase Reaction Buffer B0253SVIAL -20 1 x 1.5 ml 10 X

Companion Products

• ProtoScript® First Strand cDNA Synthesis Kit
• ProtoScript® II Reverse Transcriptase

1. Verma, I.M. (1975). J.Virol.. 15, 843-854.
2. Gerard, G.F. and Grandgenett, D.P. (1975). J.Virol.. 15, 785-797.
3. Roth, M.J., Tanese, N. and Goff, S.P. (1985). J. Biol.Chem.. 260, 9326-9335.
4. Sambrook, J., Fritsch, E.F. and Maniatis, T. (1989). Molecular Cloning: A Laboratory Manual(2nd Ed.). 5.52-5.55, 8.11-8.17.

1. First Strand cDNA Synthesis (Quick Protocol) (NEB #M0253)
2. First Strand cDNA Synthesis (Standard Protocol) (NEB #M0253)
3. First Strand cDNA Synthesis (No-RT Negative Control Reaction) (NEB #M0253)

• cDNA/Reverse Transcriptase Tips

• DNA Polymerase Selection Chart

1. Can M-MuLV Reverse Transcriptase be used in other NEBuffers?
2. Does M-MuLV Reverse Transcriptase require DTT?
3. Can the M-MuLV Reverse Transcriptase transcribe through RNA-DNA junctions?
4. How can the length of the product generated by M-MuLV Reverse Transcriptase be increased?
5. How can the yield be improved when using M-MuLV Reverse Transcriptase?
6. What are some of the reasons for M-MuLV Reverse Transcriptase reaction failure?
7. Is M-MuLV Reverse Transcriptase active at temperatures higher than 42°C?
8. Can DNA be used as a template for M-MuLV Reverse Transcriptase?
9. Are NEB DNA Polymerases supplied with dNTPs?

• Reverse Transcriptase Brochure

Quality Control tests are performed on each new lot of NEB product to meet the specifications designated for it. Specifications and individual lot data from the tests that are performed for this particular product can be found and downloaded on the Product Specification Sheet, Certificate of Analysis, data card or product manual. Further information regarding NEB product quality can be found here.

The Specification sheet is a document that includes the storage temperature, shelf life and the specifications designated for the product. The following file naming structure is used to name these document files: [Product Number]_[Size]_[Version]

• M0253S_L_v2

The Certificate of Analysis (COA) is a signed document that includes the storage temperature, expiration date and quality controls for an individual lot. The following file naming structure is used to name these document files: [Product Number]_[Size]_[Version]_[Lot Number]

• M0253S_L_v1_0281702
• M0253S_L_v1_0281706
• M0253S_L_v1_0291709
• M0253S_L_v1_0291803
• M0253L_v1_10024145
• M0253L_v1_10030253
• M0253S_v1_10029512
• M0253L_v1_10030281
• M0253S_v1_10034500
• M0253S_v1_10041931
• M0253L_v1_10040978
• M0253L_v1_10048416
• M0253S_v1_10049428
• M0253S_v1_10051884
• M0253L_v1_10051171
• M0253S_v1_10057032
• M0253L_v1_10061412
• M0253L_v2_10066881
• M0253S_v2_10064976
• M0253L_v2_10072154
• M0253S_v2_10082908
• M0253S_v2_10084233
• M0253L_v2_10094252
• M0253S_v2_10094253
• M0253L_v2_10104585
• M0253S_v2_10111027
• M0253L_v2_10113987
• M0253L_v2_10119111
• M0253S_v2_10122358
• M0253L_v2_10135008
• M0253L_v2_10107677
• M0253S_v2_10135007
• M0253L_v2_10145565
• M0253L_v2_10153266
• M0253S_v2_10153269
• M0253S_v2_10163750
• M0253L_v2_10166367
• M0253S_v2_10178660
• M0253S_v2_10187641
• M0253S_v2_10203660
• M0253L_v2_10203661
• M0253S_v2_10209405
• M0253L_v2_10209406
• M0253S_v2_10220883
• M0253L_v2_10221188
• M0253L_v2_10231822
• M0253L_v2_10246049
• M0253S_v2_10243049
• M0253L_v2_10259717
• M0253S_v2_10259718
• M0253S_v2_10268550
• M0253L_v2_10268551
• M0253S_v2_10231823
• M0253S_v2_10276401
• M0253L_v2_10277865
• M0253S_v2_10283303
• M0253S_v2_10290562
• M0253S_v2_10291534
• M0253L_v2_10290041
• M0253L_v2_10299844
• M0253S_v2_10298610
• M0253L_v2_10303854
• M0253S_v2_10303853
• M0253L_v2_10313873
• M0253S_v2_10328052
• M0253L_v2_10330069
• M0253L_v2_10342332

The following is a list of Safety Data Sheet (SDS) that apply to this product to help you use it safely.

• M-MuLV Reverse Transcriptase
• M-MuLV Reverse Transcriptase Reaction Buffer

Products and content are covered by one or more patents, trademarks and/or copyrights owned or controlled by New England Biolabs, Inc (NEB). The use of trademark symbols does not necessarily indicate that the name is trademarked in the country where it is being read; it indicates where the content was originally developed. The use of this product may require the buyer to obtain additional third-party intellectual property rights for certain applications. For more information, please email busdev@neb.com.

This product is intended for research purposes only. This product is not intended to be used for therapeutic or diagnostic purposes in humans or animals.

New England Biolabs (NEB) is committed to practicing ethical science – we believe it is our job as researchers to ask the important questions that when answered will help preserve our quality of life and the world that we live in. However, this research should always be done in safe and ethical manner. Learn more.