Phosphatases
NEB supplies a variety of phosphatases for use in cloning, such as preparation of templates for 5´end labeling, prevention of recircularization of cloning vectors, enhancing yields of RNA in transcription reactions.
Phosphatases catalyze the removal of 5´ phosphate groups from DNA and RNA (Quick Dephosphorylation Kit (NEB #M0508), Shrimp Alkaline Phosphatase (NEB #M0371), Calf Intestinal Alkaline Phosphatase (NEB #M0290), and Antarctic Phosphatase (NEB #M0289). Inorganic phosphatases catalyze the hydrolysis of inorganic pyrophosphate to form orthophosphate (Pyrophosphatase, Inorganic (yeast) (NEB #M2403) and Thermostable Inorganic Pyrophosphatase (NEB #M0296)).
NEBCloner is a guide for selecting appropriate products and viewing protocols for steps in the cloning workflow. To help select the right DNA phosphatase, choose "End Modification" on the tool to start.
NEB supplies a variety of phosphatases for use in cloning, such as preparation of templates for 5´end labeling, prevention of recircularization of cloning vectors, enhancing yields of RNA in transcription reactions.
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Choose Type:
- Phosphatase Selection Chart
- DNA Ligase Selection Chart
- Troubleshooting Guide for Cloning
- Troubleshooting Tips for Ligation Reactions
- Traditional Cloning Quick Guide
- Tips for Maximizing Ligation Efficiencies
Selection Tools
Troubleshooting Guides
Usage Guidelines
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This product is intended for research purposes only. This product is not intended to be used for therapeutic or diagnostic purposes in humans or animals.
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