Phosphatase Selection Chart
Although NEB offers different phosphatases, we recommend Quick CIP (NEB #M0525), as it has high specific activity, a rapid protocol, does not require any additives, and can be heat inactivated.
| Quick CIP (NEB #M0525) |
Recombinant Shrimp Alkaline Phosphatase(rSAP) (NEB #M0371) |
Antarctic Phosphatase (NEB #M0289) |
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|---|---|---|---|---|
| FEATURES | ||||
| 100% heat inactivation | 2 minutes/80°C |
5 minutes/65°C | 2 minutes/80°C | |
| High specific activity | ✓ |
✓ | ||
| Improved stability | ✓ |
✓ | ||
| Works directly in NEB buffers | ✓ |
✓ | ✓ | |
| Requires additive | ✓(Zn2+) | |||
| Quick Protocol (10 min) | ✓ |
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