Phosphatase Selection Chart

Although NEB offers different phosphatases, we recommend Quick CIP (NEB #M0525), as it has high specific activity, a rapid protocol, does not require any additives, and can be heat inactivated.

Quick CIP
(NEB #M0525)

Recombinant Shrimp Alkaline Phosphatase(rSAP)
(NEB #M0371)
Antarctic Phosphatase
(NEB #M0289)
100% heat inactivation 2 minutes/80°C
5 minutes/65°C 2 minutes/80°C
High specific activity

Improved stability

Works directly in NEB buffers
Requires additive

Quick Protocol (10 min)