HiFi Taq DNA Ligase
NEBU recombinant No

HiFi Taq DNA Ligase will ligate this substrate:

Nicked DNA/RNA



An optimized blend of a thermostable DNA ligase and a proprietary additive, HiFi Taq DNA Ligase efficiently seals nicks in DNA with unmatched fidelity.

  • Reduces errors for ligation-based molecular diagnostic techniques (e.g., LCR, LDR)
  • Remains active after multiple thermal cycles
  • Exhibits increased discrimination between correct and mismatched base pairs at either side of ligation junctions, making the enzyme suitable for the detection of either 3 ́ or 5 ́ mismatches (vs. other thermostable ligases which are efficient for 3 ́ mismatches only).
  • Free Thermostable Ligase ReactionTemperature Calculator tool to estimate appropriate incubation temperature
  • This product can be purchased in larger volumes. Submit an inquiry to find out more.
  • Not sure which ligase to choose? Refer to our DNA and RNA Ligase Properties Chart or DNA Ligase Selection Chart
Catalog Number Concentration Size List Price Your Price Quantity
M0647S Not Applicable 50 reactions $173.00
Please enter a quantity for at least one size

Need a custom/large volume order? Contact us

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