Monarch® RNA Cleanup Columns (50 μg)
The Monarch RNA Cleanup Columns (50 µg) are a component of the Monarch RNA Cleanup Kit (50 µg) (NEB #T2040) and can be used to purify up to 50 µg of RNA from enzymatic reactions. They are supplied with Monarch Collection Tubes II (NEB #T2018). RNA Cleanup Binding Buffer (NEB #T2041) and Monarch RNA Wash Buffer (NEB #T2042) are also available separately.
- Ideal for the cleanup of RNA from enzymatic reactions including sgRNA synthesis, DNase I treatment, labeling, and capping.
- Elute in ≥ 20 µl for concentrated RNA
- 70-100% RNA recovery
- Unique column design prevents buffer carryover and elution of silica particulates
- Purified RNA is ready for use in a wide variety of downstream applications
Monarch® RNA Cleanup Protocol
Tips for successful RNA Cleanup using the Monarch® RNA Cleanup Kits
Avoiding RNase Contamination
How to recycle your Monarch Kit components
The Monarch RNA Cleanup Columns (50 µg) are a component of the Monarch RNA Cleanup Kit (50 µg) (NEB #T2040) and can be used to purify up to 50 µg of RNA from enzymatic reactions including DNase treatment (NEB #M0303), labeling, and capping. The columns are ideal for cleaning up RNA after in vitro transcription reactions such as sgRNA synthesis reactions (NEB #E3322). The columns are designed without the use of a retaining ring, ensuring no buffer retention and no carryover of contaminants. RNA can be eluted in as little as 20 μl for and is ready for use in a variety of downstream applications including RT-PCR, RNA Library Prep for NGS and transfection.
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